BRET-based biosensors for SARS-CoV-2 oligonucleotide detection حسب Asfia Sultana (19170880)

"…Specifically, we designed BioODs against the SARS-CoV-2 parental (Wuhan strain) and B.1.617.2 Delta variant through the conjugation of specific, fluorescently modified molecular beacons (sensor module) through a complementary oligonucleotide handle DNA functionalized with the NanoLuc (NLuc) luciferase protein such that the dissolution of the molecular beacon loop upon the binding of the viral oligonucleotide will result in a decrease in BRET efficiency and, thus, a change in the bioluminescence spectra. …"

BRET-Based Biosensors for Nucleic Acid Detection and Impact of Molecular Crowding Monitoring حسب Asfia Sultana (19170880)

"…Our work extended to designing BioNADs targeting SARS-CoV-2 variants by conjugating specific, fluorescently modified molecular beacons to NLuc-functionalized DNA for viral nucleic acid detection. …"