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    A BRET-based Mpro biosensor containing a nanobody and tandem cleavage sites shows an increased cleavage rate

    A BRET-based Mpro biosensor containing a nanobody and tandem cleavage sites shows an increased cleavage rate by Geethakumari, Anupriya M

    Published 2025
    “…Importantly, the inclusion of an Mpro-binding, but non-inhibiting, NB2E3 nanobody at the N-terminal further increased the cleavage rate of the 2× cleavage sequence-containing Mpro biosensor. We envisage that the NB2E3 nanobody-2× Mpro biosensor engineered here will be useful in drug discovery and functional characterization of Mpro mutants in newly emerging SARS-CoV-2 variants as well as in detecting SARS-CoV-2 infection in a point-of-care testing (POCT) format.…”
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