Confirmation of miRNA level by RT-qPCR. by Qin-Min Ge (3838885)

“…<p>Relative expression of each miRNA was normalized to hsa-miR-423-5p level. miR-142-5p, miR-22-3p, miR-191-5p, miR-23a-3p and miR-4456 were significantly decreased in both the sepsis-induced AKI and sepsis-non AKI groups compared with the healthy controls. miR-4270, miR-4321 and miR-3165 were obviously increased in the sepsis-induced AKI, and miR-3165 also was obviously increased in sepsis-non AKI group compared with the healthy controls. …”

microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration - Fig 6 by Surjyadipta Bhattacharjee (3181674)

“…After vacuum evaporation of HFIP, aliquoted peptide was re-suspended with DMSO to 5 mM and diluted to 5 μM into the cell culture media; <b>(B)</b> cells were treated with a scrambled miRNA-34a sequence (miRNA-34a-<b>sc</b>; 30 nM;, control, <b>a-d</b>, middle row); or <b>(C)</b> with an LNA-stabilized miRNA-34a (30 nM; miRNA-34a stressed; <b>a-d</b>; bottom row); treatments were for 24 hr before incubation with 5 uM of Aβ42 (made up as in [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref033" target="_blank">33</a>]) for another 24 hr before assay; MG cells were subsequently stained using a murine amyloid beta MABN10 (red fluorescence λ_max~650 nm; anti-Aβ antibody, clone W0-2; Millipore, Bellerica MA), a TREM-2 antibody (M-227): sc-48765 (green fluorescence; λ_max~510 nm; Santa Cruz, Santa Cruz CA) or DAPI nuclear stain (blue fluorescence; λ_max~470 nm) as in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a>;</b> arrows indicate Aβ42 uptake into MG cells; note decreased presence of TREM2 in miRNA-34a treated MG cells (bottom row, panel B) and decrease in ingested Aβ42 peptide within C8B4 cells (<b>C</b>; bottom row, panel <b>d</b>). …”

Characterization of serum cytokines during systemic infection with an <i>fgr41Δ/Δ</i> mutant. by Andrew S. Wagner (11331477)

Conditional <i>Cdk5rap2</i> KO mouse and novel <i>mCdk5rap2</i> splice variants. by Nadine Kraemer (790327)

“…(D) Identification of a novel <i>mCdk5rap2</i> splice variant (<i>mCdk5rap2-V1</i>) through gel electrophoresis and sequencing of PCR products from exon 2 to exon 7 of WT and hom KO cDNA. …”

Analysis of the various EcFicTA complexes in solution. by Frédéric V. Stanger (585551)

Table_5_Bacillus velezensis B105-8, a potential and efficient biocontrol agent in control of maize stalk rot caused by Fusarium graminearum.DOCX by Shuang Wang (46453)

“…S-NP activity showed an initial increase with a peak of 20,337 nmol/h/g, followed by a decrease, but activity remained significantly better than control treatment with chemical fungicides. …”

A large proportion of NSCs redivide within 24 to 72 h. by Valerio Lupperger (9745714)

“…(B–E) Example DLS (yellow arrow) for a labeling interval Δt = 9 h. (F) The DLS proportion is high for Δt = 9 h and decreases rapidly with increasing Δt. …”

Fast Pyrolysis of Tropical Biomass Species and Influence of Water Pretreatment on Product Distributions by Trevor James Morgan (787777)

“…The effect of pretreatment (i.e. reducing the amount of ash, and alkali and alkali earth metals) on pyrolysis products is: 1) to increase the dry bio-oil yield, 2) to decrease the amount of undetected material, 3) to produce a slight increase in CO yield or no change, 4) to slightly decrease CO₂ yield or no change, and 5) to produce a more stable bio-oil (less aging). …”

Loss of <i>FGR41</i> or <i>CWP419</i> does not attenuate fungal growth <i>in vitro</i>. by Andrew S. Wagner (11331477)

Irradiating the lungs of young adult female FVB/N mice with either a single 12 Gy dose or fractionated 24 or 30 Gy doses of ¹³⁷Cs γ-rays leads to decreased levels of E-c... by Celine Almeida (289132)

“…<b>B, D</b>: After a fractionated 24 Gy dose, there is a slight increase in E-cadherin at 1 month postirradiation (PI) followed by a progressive decrease up to 5 months PI. …”

Orthostatic Hypotension and Elevated Resting Heart Rate Predict Low-Energy Fractures in the Population: The Malmö Preventive Project by Viktor Hamrefors (512698)

“…</p><p>The median follow-up time from baseline to first incident fracture among the subjects that experienced a low energy fracture was 15.0 years. A 10 mmHg orthostatic decrease in systolic blood pressure at baseline was associated with 5% increased risk of low-energy-fractures (95% confidence interval 1.01–1.10) during follow-up, whereas the resting heart rate predicted low-energy-fractures with an effect size of 8% increased risk per 10 beats-per-minute (1.05–1.12), independently of the orthostatic response. …”

Dynamics of different T-cell subpopulations and estimated production rates including confidence intervals. by Sara P. H. van den Berg (8703927)

Characteristics of the microbial composition between resistance types. by Astri Dewayani (12878143)

SHOULD MALE CIRCUMCISION BE PROMOTED IN DEVELOPING COUNTRIES AS MAJOR MEANS OF HIV PREVENTION by Wazhma Hakimi.pdf by Wazhma Hakimi (8820506)

“…However, male circumcision (MC) has been known as a religious or traditional ritual, it was suggested as a mean for prevention of sexually transmitted infections (STIs) in 1850s. …”

Synonymous Rare Arginine Codons and tRNA Abundance Affect Protein Production and Quality of TEV Protease Variant by Jie Fang (306330)

“…<div><p>It has been identified that a TEV protease (TEVp) variant, TEVp^5M, displays improved solubility. …”

Alpha- and beta-defensins are not relevant for polyP dependent gonococcal infection. by Benedetta Manca (15810991)

The interaction of <i>F</i>. <i>tularensis</i> CipR and <i>kdsD</i>::<i>ltrB</i>_<i>L1</i> mutants with macrophage-like cells show a decrease in CFU recovery and disrupti... by Taylor Chance (611108)

Three-dimensional flow field of the Trp-cage folding. by Vladimir A. Andryushchenko (4662829)

Subject characteristics. by Astri Dewayani (12878143)

“…Following resistance status to five antibiotics, samples were classified into 24 sensitive, 24 single resistance, 16 double resistance, 5 triple resistance. …”

Data_Sheet_5_Influence of CO2 Degassing on the Microbial Community in a Dry Mofette Field in Hartoušov, Czech Republic (Western Eger Rift).CSV by Qi Liu (33068)