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19841
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19842
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19843
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19844
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19845
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19846
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19847
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19848
Programmed Release METTL3-14 Inhibitor Microneedle Protects Myocardial Function by Reducing Drp1 m6A Modification-Mediated Mitochondrial Fission
Published 2023“…RIP assay and mechanistic investigation confirmed that METTL3 inhibition reduced the translation efficiency of Drp1 mRNA by 5′UTR m6A modification, thus decreasing the Drp1 protein level and mitochondrial fragment after hypoxic-ischemic injury. …”
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19849
Functional validation of a miRNA-34a-TREM2–3’UTR interaction.
Published 2016“…<p><b>(A)</b> ribonucleotide sequence of the 299 nt TREM2-mRNA-3’-UTR is shown in the 5’-3’ direction; the 22 nt miRNA-34a-TREM2 3’UTR complementarity-interaction region is indicated by a black underline and the 8 nt TREM2-mRNA-3’-UTR seed sequence <b>5’-ACACUGCU-3’</b> is overlaid in yellow; a single arrowhead indicates the 5’ end of a poly A+ tail in the TREM2 mRNA (22 ‘A’ nt shown; the length of this poly A+ tail is variable); TREM2 mRNA sequence derived from NM_018965; TREM2 transcript is the major X1 variant (see also <a href="http://switchdb.switchgeargenomics.com/productinfo/id_801321/" target="_blank">http://switchdb.switchgeargenomics.com/productinfo/id_801321/</a>) (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a></b>); <b>(B)</b> TREM2-mRNA-3’UTR expression vector luciferase reporter assay (pLightSwitch-3’UTR; Cat#S801178; Switchgear Genomics, Palo Alto CA); in this vector, the entire 299 nucleotide TREM2 3’UTR was ligated into the unique Nhe1-Xho1 site; <b>(C)</b> control C8B4 murine microglial cells, 1 week in culture; phase contrast bright field microscopy 20x; C8B4 cells transfected with the TREM2-mRNA-3’UTR expression vector luciferase reporter were treated exogenously with miRNA-34a, a scrambled control miRNA-34a (miRNA-sc) or control miRNA-183; see references and text for further details [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref018" target="_blank">18</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref019" target="_blank">19</a>]; <b>(D)</b> compared to control, C8B4 cells transfected with a scrambled (<b>sc</b>) control pLightSwitch-3’UTR vector, the TREM2-mRNA-3‘UTR vector exhibited decreased luciferase signal to a mean of 0.16-fold of controls in the presence of miRNA-34a; this same vector exhibited no change in the presence of the control miRNA-34a-sc or miRNA-183; for each experiment (using different batches of MG cells) a control luciferase signal was generated and included separate appropriate controls with each analysis; in addition a control vector β-actin-3’UTR showed no significant effects on the relative luciferase signal yield after treatment with either miRNA-183 or miRNA-34a (data not shown); dashed horizontal line set to 1.0 for ease of comparison; N = 5; *<i>p</i><0.001 (ANOVA). …”
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19850
Influence of chemical components of PVAC during storage of RBC.
Published 2019“…In <b>(b)</b> values after 1 month (end of storage) are shown: PVA at 100 μg/mL increased hemolysis while PVAC at 2.5 mg/mL, 500 and, 100 μg/mL decreased the levels of hemolysis compared to the control.…”
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19851
Data_Sheet_1_MiR-30c-5p/ATG5 Axis Regulates the Progression of Parkinson’s Disease.xlsx
Published 2021“…MiR-30c-5p antagomiR attenuated the decrease of ATG5 in PD mice. …”
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19852
<i>nt5e</i> confers Nt5e activity on <i>S. sanguinis</i> SK36 whole cells.
Published 2012“…(A), (B), and (C) were showed as enzyme velocity vs. concentration of ATP, ADP and AMP substrates, where the results were represented as mean±SE, n = 3. …”
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19853
Histamine 1 Receptor Blockade Enhances Eosinophil-Mediated Clearance of Adult Filarial Worms
Published 2015“…Although fexofenadine-treated mice had decreased antigen-specific IgE levels as well as lower splenocyte IL-5 and IFNγ production, they exhibited a greater than fourfold rise in eosinophil numbers at the tissue site where adult <i>L</i>. …”
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19854
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19855
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19856
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19857
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19858
Image_1_Loss of the Essential Autophagy Regulators FIP200 or Atg5 Leads to Distinct Effects on Focal Adhesion Composition and Organization.TIF
Published 2020“…We provide evidence suggesting the primary autophagy regulators Atg5 and FIP200 both play a role in cell motility and extracellular matrix adhesion. …”
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19859
Image_2_Loss of the Essential Autophagy Regulators FIP200 or Atg5 Leads to Distinct Effects on Focal Adhesion Composition and Organization.TIF
Published 2020“…We provide evidence suggesting the primary autophagy regulators Atg5 and FIP200 both play a role in cell motility and extracellular matrix adhesion. …”
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19860
Data_Sheet_1_Five-year measurement data along a 1200 m elevational gradient reveals that global warming increases soil respiration.PDF
Published 2023“…To clarify the effects of soil temperature and volumetric soil water content on soil respiration rates (R<sub>S</sub>), we examined seasonal changes in R<sub>S</sub> at five elevations of 1600–2800 m in subalpine coniferous forests in Japan for 5 years. …”