High doses of exogenous glucagon stimulate insulin secretion and reduce insulin clearance in healthy humans by Sarah M. Gray (295663)

“…To test the hypothesis that the relative effects of glucagon on hepatic and islet function were also varied with blood glucose, a group of healthy subjects received glucagon (100 ng/kg) during fasting glycemia or experimental hyperglycemia (~150 mg/dl) on two separate days. …”

Systemic antioxidant treatment and intermittent fasting delay axonal degeneration in mice. by Andrea Calixto (16070)

“…The neurofilament signal decrease progressively at 3, 5 and 7 days after nerve injury in non-treated mice (A). …”

S1 Fig - by Magdalena Miranda (14667161)

“…Individual values used to calculate the mean and SEM are presented as dots. The data that support these findings is available in OSF at <a href="https://osf.io/7pw23/" target="_blank">https://osf.io/7pw23/</a>.…”

Zinc supplementation reduces macrophage IL-10 production in response to LPS. by Charlie J. Pyle (3617798)

“…<p>Zinc co-administration during LPS (100 ng/mL) stimulation of macrophages at 6 h decreases (<b>A</b>) IL-10 but increases (<b>B</b>) TNFα, (<b>C</b>) IL-8, and (<b>D</b>) IL-6 mRNA expression as determined by qRT-PCR (experimental groups quantified relative to GAPDH and normalized to resting controls). …”

Supplementary Material for: Hepcidin removal during Continuous Renal Replacement Therapy by Colbert J.F. (17330053)

Comparative analysis of the in vitro concerted integration catalyzed by HIV-1 IN, HIV-1 IN/LEDGF and HIV-1 IN/LEDGF/INI1-IBD complex. by Benoit Maillot (401506)

“…Concerted integration assay was performed using 0.1 to 12.5µM IN or IN/LEDGF complex (concentration are normalized to IN monomers), 150 ng of acceptor plasmid, 15 ng of radioactively labelled and processed donor DNA. …”

Analysis of Ub-X-ZFNs. by Shondra M. Pruett-Miller (374440)

“…(E) Toxicity assay for all iterations of Ub-modified and unmodified ZFNs tested in the gene targeting assay relative to Sce. A value of <100% indicates decreased cell survival as compared with Sce, and demonstrates a toxic effect. …”

Other iron chelators also reduce ROS production by PMNs. by Armand Paauw (255206)

Exposure to particle debris generated from passenger and truck tires induces different genotoxicity and inflammatory responses in the RAW 264.7 cell line by Anna Poma (835467)

“…In MTS assay, after 24 hours, it was found that PP induced a 30% decrease in metabolic activity at a concentration of 10 μg/ml, while TP caused reductions of 20% and 10% at concentrations of 10 μg/ml and 50 μg/ml, respectively. …”

Incidence of Type 1 Diabetes in Relation to Exposure to Rotavirus Infections in Pre- and Post-Vaccine Birth Cohorts in Finland by Anna Parviainen (9343391)

“…</p><p dir="ltr">Conclusions At the population level, a decrease in exposure to rotavirus infections was associated with a decrease in the incidence of type 1 diabetes in young child.…”

Activation of caspases and mitochondria. by Michael Plötz (326888)

“…SK-Mel-13 cells had been transduced with AdV-AK (MOI=100, 48 h). Means and SDs of triplicate values of a representative experiment (one of two) are shown. …”

ENMD-1198 inhibits c-Src-mediated STAT3 activation. by Saad Yousuf (541082)

“…Some cells were treated with 40 ng/ml IL-6 for 6 h. Cells were harvested, washed and lysed in NP-40 lysis buffer. …”

Study the effect of 3,4-Methylenedioxy methamphetamine on cytochrome P450 2E1 activity by Shahin Nilchi (14282329)

Halogenated organic pollutants (HOPs) in marine fish collected from central-northern coastal waters in Vietnam by Nam Vu Duc (17759979)

“…The concentration of analytes was quantified by internal standard method and controlled with corresponding label standards. The decreasing order of HOP concentrations was ∑20OCPs (mean/median: 158/139 ng/g-wet weight (ww), range: 31.1-406 ng/g-ww) > ∑28PCBs (mean/median: 46.6/36.5 ng/g-ww, range: 5.92-225 ng/g-ww) > ∑8PBDEs (mean/median: 28.7/4.76 ng/g-ww, range: 0.26-212 ng/g-ww). …”

Injection of wild-type H3.3 RNA and reduction of mutant H3.3 levels both rescue craniofacial skeletal development in <i>h3f3a^db1092</i> mutants. by Samuel G. Cox (135948)

“…<p>a, b, Results from scoring the severity of craniofacial losses in 5 dpf larval head skeletons from <i>h3f3a^db1092</i> siblings injected with 450 ng/µl mRNA encoding wild-type H3.3 or a control kikGR fluorescent protein. …”

Sensitivity of the newly designed primer. by Chaojen Wang (419539)

“…<p>Agarose gel showing the sensitivity of polymerase chain reaction (PCR) using the genomic DNA of a nonpathogenic strain of <i>Fusarium oxysporum</i> and the primer pair FIGS11/NPIGS-R: Amplification of a decreasing amount of the nonpathogenic isolate Fo276 DNA ranging from 200 to 10^-3 ng. …”

Mechanical load represses transactivation by Smad2/3 in tibial osteocytes. by Jacqueline Nguyen (290539)

“…UMR-106 cells were harvested 2 h after treatment with vehicle (DMSO), TGFβ1 (5 ng/ml), or an inhibitor of the TGFβ type 1 receptor, Alk5 (TβRI-I, SB431542). …”

Clinical improvement of juvenile macaques with chronic diarrhea following <i>T. trichiura</i> treatment. by Mara Jana Broadhurst (120227)

NF-κB inhibition with Compound A enhances the cytotoxicity of doxorubicin through increased apoptosis. by Brian K. Bednarski (259893)

“…<p>(A) U2OS cells were seeded in 6-well plates and treated every 48 hours with DMSO, doxorubicin (Dox, 5 ng/ml), Compound A (Cmpd A, 5 µM), or Dox+Cmpd A. …”

The association between ROCK1 expression and clinical outcomes. by Ji-Young Shin (204248)

“…ROCK1 siRNA or combined ROCK1 siRNA and miRNA-135a inhibitors decrease cell migration (p = 0.003) and invasion (p = 0.017). …”