Feasibility of Large-Scale Phosphoproteomics with Higher Energy Collisional Dissociation Fragmentation by Nagarjuna Nagaraj (1296663)

“…Reducing the target values from a standard of 30 000 to 5000 ions did not severely affect identification rates but did decrease identification and localization scores for phosphorylation sites. …”

Feasibility of Large-Scale Phosphoproteomics with Higher Energy Collisional Dissociation Fragmentation by Nagarjuna Nagaraj (1296663)

“…Reducing the target values from a standard of 30 000 to 5000 ions did not severely affect identification rates but did decrease identification and localization scores for phosphorylation sites. …”

Expression of STUB1DN mutants leads to the formation of protein aggregates. by Joao Vasco Ferreira (7499918)

“…Cells were further incubated in the presence or absence of 10uM of MG-132 and 50nM of BafA for 6h. A) Cell extracts of proteins insoluble in increasing concentrations of SDS where filtered through a nitrocellulose membrane and trapped proteins were blotted with antibodies directed against ubiquitin, p62 and myc tag. …”

Feasibility of Large-Scale Phosphoproteomics with Higher Energy Collisional Dissociation Fragmentation by Nagarjuna Nagaraj (1296663)

“…Reducing the target values from a standard of 30 000 to 5000 ions did not severely affect identification rates but did decrease identification and localization scores for phosphorylation sites. …”

Simultaneous PreCaT and extracellular field potential recordings. by Carmelo Sgobio (652457)

“…Effects of application of a cocktail of antagonists of ionotropic glutamate and GABA receptors ((2R)-amino-5-phosphonovaleric acid (APV, 50 µM); 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBXQ, 10 µM)) are also shown. …”

Female motorcycle mortality in Brazilian municipalities, 2005, 2010 and 2015 by Pedro Cisalpino Pinheiro (6070499)

“…In the municipalities of the South and Southeast regions, mainly in the period between 2010 and 2015, there was an apparent decrease in mortality. The percentage variation showed a reduction in the indicator analyzed in the period between 2010 and 2015 for the largest municipalities in the South and Southeast regions. …”

Neutralization by PGT121-137 antibodies and chimeric variants. by Marie Pancera (335526)

“…<p>Strong neutralization is coded in red (>90%), intermediate in yellow (75–90%) and weak in green (50–75%). Neutralization data were generated in single neutralization experiments, with each test sample analyzed in duplicate to determine the percent decrease in virus growth (neutralization) as compared and normalized to the control wells without antibody expression (defined as 0% neutralization). …”

H35N induced cell death and changes in nuclear morphology in presence of pan-caspase inhibitor zVADfmk: by Saumya S. Srivastava (262013)

“…<p>(A) A431 cells either left untreated or treated with H35N (8 µg/ml) or zVADfmk (50 µM) for 2 hr followed by addition of H35N (8 µg/ml) for the indicated time which were then mildly trypsinised and viable cells were counted after staining with trypan blue as mentioned in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006293#s2" target="_blank">materials and methods</a>. …”

Schematic of the filtration setup and flowmeter device used in this study. by Corentin Fournier (5088941)

“…<p>We used overpressure filtration for plankton size-class filtration with three filters in series, (<i>i</i>) a 180-μm nylon net pre-filter to remove zooplankton and larger particles, and (<i>ii</i>) a 5.0-μm and (<i>iii</i>) a 0.2-μm pore-size polycarbonate membrane filter, in order to collect the nanoplankton and picoplankton, respectively, for DNA extraction. …”

qPCR analysis of rat EMCs and mouse heart explants treated with ICG-001. by Tomoyo Sasaki (87849)

“…(F) ICG-001 significantly decreased the expression of <i>Wt1</i> in rat EMCs. …”

Data presented in the paper "Consumer and host body size effects on the removal of trematode cercariae by ambient communities" by J. (Jennifer) Welsh (9158432)

“…One individual was The effect of dilutor size on removal rates of cercariae was investigated in laboratory experiments by determining removal rates of five size categories of each dilutor species (Table 1). In addition, a sixth treatment without dilutors served as a control to account for potential loses of cercariae due other factors (knowing the number of added cercariae was 50) and to test for the presence of a dilution effect (control vs. dilutor size treatments). …”

Red Blood Cell Transfusion and Mortality in Trauma Patients: Risk-Stratified Analysis of an Observational Study by Pablo Perel (102217)

“…Transfusion was associated with an increase in all-cause mortality among patients with &lt;6% and 6%–20% predicted risk of death (odds ratio [OR] 5.40, 95% CI 4.08–7.13, <i>p&lt;</i>0.0001, and OR 2.31, 95% CI 1.96–2.73, <i>p&lt;</i>0.0001, respectively), but with a decrease in all-cause mortality in patients with &gt;50% predicted risk of death (OR 0.59, 95% CI 0.47–0.74, <i>p&lt;</i>0.0001). …”

Data_Sheet_1_Molybdenum and Cadmium Co-induce Mitochondrial Quality Control Disorder via FUNDC1-Mediated Mitophagy in Sheep Kidney.doc by Yunhui Wu (2995374)

“…Forty-eight 2-month-old sheep were stochastically divided into four groups, as shown below: control group, Mo [45 mg/kg body weight (BW)] group, Cd (1 mg/kg BW) group and Mo (45 mg/kg BW)+Cd (1 mg/kg BW) group, with 50 days feed technique. The results showed that Mo or/and Cd attract an unbalance of trace minerals and vacuoles and granular degeneration of renal tubular epithelial cells, and increase the number of mitophagosomes and vacuole-mitochondria and LC3 puncta and MDA and H₂O₂ contents, and decrease ATP content in the kidney. …”

Equilibrium binding of single strand DNA (P1) from promoter with mNdK by Praveen Kumar (8242)

“…CD spectra of P1 (4 μM) shows decrease in ellipticity at 285 nm with increasing mNdK (0–0.5 μM) in 50 mM Tris-MES-acetate pH 6.5, 1 mM MgCl, 75 mM NaCl within 30 min of incubation, after each addition, at 37°C. () Enzymatic (phosphotransfer) activity of mNdK at pH 6.5. …”

HepR21 cells have increased resilience to nutrient deprivation compared to HepG2 cells. by Paramita Saha (473954)

“…A set of cells were kept as control. The survivability of HepR21 cells was found to remain unaffected while HepG2 cells were highly sensitive upon prolonged starvation with an overall 50% decrease in growth after 36 h of starvation. …”

Predictive validity. by Roberta Maria Lorenzi (16920886)

“…Simulations last 500 ms with a time resolution of 0.1 ms and <i>υ</i>drive is a step at 50 Hz lasting 250 ms starting at 125 ms. …”

Video_1_Robustness and Scalability of Regional Climate Projections Over Europe.mp4 by Dominic Matte (6270914)

“…<p>Climate change projections for Europe consistently indicate a future decrease in summer precipitation over southern Europe and an increase over northern Europe. …”

Forage yield and structural responses of spineless cactus ‘Orelha de Elefante Mexicana’ at different planting densities by Juliana de Souza Pereira (10442366)

“…The evaluated densities were 20,000; 25,000; 33,333; 50,000; and 100,000 plants ha-1. The increase in planting density caused a linear reduction in plant height, width, and mass, as well as in number of primary, secondary, tertiary, and overall cladodes. …”

Increased proteolysis of YY1 is observed in post-mortem Alzheimer’s disease (AD) brain tissue. by Soline Aubry (507157)

“…Densitometric analysis of immunoblots from BA38 shows (<i>C</i>) a significant decrease (<i>p</i> = 0.02) in the full-length YY1 protein (60 kDa) in AD (<i>n</i> = 15, mAD + sAD) and (<i>D</i>) a significant increase (<i>p</i> = 0.002) in the proportion of proteolytic fragments (35–50 kDa) compared to controls (<i>n</i> = 9). …”

NF-κB activity is required for head formation. by Neil J. Armstrong (323599)

“…(<b>D</b>) Graphical representation of dorsoanterior index (DAI) of control embryos (blue columns) versus embryos injected dorsally with 50 pg of ΔNIκBα (red columns). (<b>E</b>) Time course analysis of embryos injected dorsally with a total of 500 pg ΔNIκBα RNA at the two, four, eight, 16- and 32-cell stage; the accompanying decrease in cell size was compensated by injecting multiple dorsal cells from the 8-cell stage on (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036136#s4" target="_blank">materials and methods</a>). …”