Processing kinetics with wild type tRNase Z and Phe329Ala variant. by Makenzie Saoura (4521427)

“…(C-D) Michaelis-Menten kinetics experiments were performed using constant concentration of ³²P labeled substrate with added unlabeled substrate varied over a concentration range from 4.8–120 nM (shown below gel panels), with reactions incubated at 28°C and sampled after 5, 10, and 15 minutes. WT and F329A enzyme concentrations (shown above gel panels) were adjusted to obtain roughly equivalent product in the variant, here requiring an almost 1,000-fold higher concentration of F329A variant than WT. …”

FOXC1 modulates MYOC secretion through regulation of the exocytic proteins RAB3GAP1, RAB3GAP2 and SNAP25 by Alexandra Rasnitsyn (4062982)

“…In addition, we found that FOXC1 regulation of RAB3GAP1, RAB3GAP2 and SNAP25 affects secretion of Myocilin (MYOC), a protein associated with juvenile onset glaucoma and steroid-induced glaucoma. …”

DataSheet_1_Assessing spatial and temporal variation in obligate resprouting, obligate seeding, and facultative seeding shrub species in California’s Mediterranean-type climate reg... by Emma C. Underwood (10489289)

“…Second, we assessed the spatial variability using (a) moisture availability (climatic water deficit and solar radiation) and found FS accounted for an increasingly greater proportion of total aboveground live biomass as water became limited, while OR biomass decreased; and (b) ecosystem productivity (NDVI) which showed the proportion of OS, OR, and tree biomass increased with productivity (with a corresponding dramatic decrease in herb biomass). …”

Predictors of 30-day mortality and the risk of recurrent systemic thromboembolism in cancer patients suffering acute ischemic stroke by Ki-Woong Nam (3829753)

“…</p><p>Aim</p><p>In this study, we evaluate the possibility of D-dimer as a predictor of 30-day mortality in stroke patients with active cancer.…”

Mst1^−/− mice exhibit blunted MOG-specific proliferative and effector cytokine recall responses in vivo. by Konstantin V. Salojin (567844)

“…(<b>D</b>) Mice treated with an Mst1 inhibitor exhibit decreased severity of EAE. EAE was induced in C57Bl/6-Albino/129SvEv mice (n = 10 per treatment group) by immunization with MOGp35–55 in CFA and treated twice daily with the indicated doses of the Mst1 inhibitor LP-945706 administered by oral gavage in citrate buffed (pH 4.5, 0.1 M) 1% Tween 80 vehicle, starting one day after the onset of disease in 50% of the animals. …”

The majority of spatial information in astrocytes is genuine spatial information that cannot be explained by tuning to visual cues. by Sebastiano Curreli (12192031)

“…<p><b>(A)</b> Fraction of astrocytic ROIs encoding reliable spatial information showing a significant decrease in their information content when position is shuffled within the same visual cue (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3001530#sec003" target="_blank">Methods</a>). …”

Design, antimicrobial activity and mechanism of action of Arg-rich ultra-short cationic lipopeptides by Federica Armas (3626381)

“…<i>aureus</i> even at sub MIC concentrations and induced cell membranes permeabilization by producing visible cell surface alterations leading to a significant decrease in bacterial viability. …”

Table_1_Angiotensin System Autoantibodies Correlate With Routine Prognostic Indicators for COVID-19 Severity.docx by Carmen M. Labandeira (12217994)

“…</p>Results<p>Hemoglobin, lactate dehydrogenase and procalcitonin were selected. A decrease in one unit of hemoglobin, an increase in 0.25 units of procalcitonin, or an increase in 100 units of lactate dehydrogenase increased the severity of the disease by 35.27, 69.25, and 3.2%, respectively. …”

anti-FT antibody POM2 counteracts prion-induced neurotoxicity in COCS. by Uli S. Herrmann (124972)

“…Because of the different electrophoretic patterns of POM2-treated samples, we have opted to perform a quantification of the entire lane (right panel). …”

DataSheet1_Projected Changes in Precipitation Extremes Over Jiulongjiang River Basin in Coastal Southeast China.pdf by Chang Li (133800)

“…However, a remarkable decrease in RL50yr_RX5day is found in the south basin in the far future. …”

Biochemical characterization of the endolysins of the lactococcal phages P008, c2, 1358, and P335. by Frank Oechslin (4825476)

“…The concentrations used corresponded to their specific activities, which were defined as the amount of enzyme needed to decrease the absorbance by 50% in 15 min (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3001740#pbio.3001740.s016" target="_blank">S1 Data</a>). …”

Identification of osmoadaptive strategies in the halophile, heterotrophic ciliate <i>Schmidingerothrix salinarum</i> by Lea Weinisch (4786002)

The effect of rottlerin on MACC1 expression. by Manisha Juneja (4059493)

“…MACC1 mRNA expression was analyzed by qRT-PCR. Samples with a 50% decrease in MACC1 mRNA levels are highlighted with black bars. …”

Identification of host factors required for HCV RNA replication or IRES-mediated translation. by Qisheng Li (522711)

“…Values relative to siNT in the heatmap are depicted in a continuum of blue (reduced infection - more than 50% decrease of the replicon activity after siRNA-mediated silencing; proviral) to red (enhanced infection - more than 50% increase of the replicon activity after siRNA-mediated silencing; antiviral). …”

<i>De Novo</i> Assembly of Human Herpes Virus Type 1 (HHV-1) Genome, Mining of Non-Canonical Structures and Detection of Novel Drug-Resistance Mutations Using Short- and Long-Read... by Timokratis Karamitros (2839292)

“…Hybrid genome assembly resulted in a decrease in the number of contigs in 6 out of 7 samples and an increase in N(G)50 and N(G)75 of all 7 samples sequenced by both technologies. …”

Household air pollution and risk of pulmonary tuberculosis in HIV-Infected adults by Patrick D.M.C. Katoto (17807086)

“…</p><p dir="ltr">We recruited 435 cases and 842 controls (median age 41 years, [IQR] 33-50; 76% female). …”

Supplementary Material for: Peridialytic Erythropoietin versus Roxadustat in Hemodialysis-dependent CKD Patients by figshare admin karger (2628495)

“…A propensity score-matched analysis (1:1 ratio; caliper width: 0.02) was conducted to minimize the impact of confounding factors. …”

Grain Yield and Quality of Foxtail Millet (<i>Setaria italica</i> L.) in Response to Tribenuron-Methyl by Na Ning (622994)

“…An increase in TBM dosage led to a decrease in grain Mn, Cu, Fe, and Zn concentrations. …”

Regulation of the Inevitable Water-Responsivity of Silk Fibroin Biopolymer by Polar Amino Acid Activation by Woojin Choi (610810)

“…Furthermore, the water dynamics study suggested that active amino acid stabilizes crystallization-triggering water molecules. As a proof-of-concept, we verified that a 30% increase in the activity of serine resulted in a 50% decrease in the crystallization rate. …”

Data_Sheet_1_Irisin Stimulates the Release of CXCL1 From Differentiating Human Subcutaneous and Deep-Neck Derived Adipocytes via Upregulation of NFκB Pathway.DOCX by Abhirup Shaw (11545129)

“…Gene expression data indicated upregulation of the NFκB pathway upon irisin treatment, which was validated by an increase of p50 and decrease of IκBα protein level, respectively. …”