Comparative interaction study of elisidepsin and the plasma membrane of HCT-116 and HCT-116-Irv cells. by José Manuel Molina-Guijarro (814644)

“…Pink mask and pink cursor correspond to elisidepsin complexes, containing elisidepsin, Irv-OG488 and Irv-A555 molecules at distances shorter than 50 Å, mainly located in the plasma membrane of affected cells. …”

Mutagenesis of the CXCL10 binding site on <i>L</i>. <i>(L</i>.<i>) major</i> GP63 to <i>L</i>. <i>(V</i>.<i>) panamensis</i> residues reduces CXCL10 cleavage and T cell chemotaxis. by Alejandro L. Antonia (7147739)

“…Equal amounts of GP63 protein from each mutant were incubated with 50 mg/mL of azocasein for 24 hours. Cleaved azocasein was determined by measuring OD440 above background determined by a no transfection media control. …”

FFA profile in <i>cpdm</i> and WT epidermis by MRM-profiling. by Jackeline Franco (5146820)

“…<p>(A) Relative amounts of polyunsaturated FFAs were increased and saturated and monounsaturated FFAs were decreased in the <i>cpdm</i> epidermis. (B) The relative amounts of FFAs with chain length of 12–18 and longer than 26 carbons were reduced in <i>cpdm</i> samples compared to WT, instead FFAs with 22–24 carbons were increased. …”

Data Set - Management and outcomes of severe dengue patients presenting with sepsis in a tropical country by Direk Limmathurotsakul (753652)

“…Values are Yes, No and N/A. N/A means that the information was not available.</p><p>12. …”

De-repression of endogenous retroelements triggers viral mimicry, interferon, and cytokine response. by Sarfraz Shafiq (13259589)

“…<p><b>(A)</b> Volcano plot of ATAC-seq results for sequence repeats with increased (blue) and decreased (red) chromatin accessibility in ATRX-null microglia. …”

Continuous Flow Synthesis of Ethyl Diazoacetate and CFD Analysis of Micromixing Efficiency in a Microreactor by Nan Wang (21935)

“…In the present study, the continuous flow synthesis and separation of diazoacetate ethyl ester (EDA) in a microchannel reactor were investigated using glycine ethyl ester hydrochloride (GEE), sodium nitrite, and acetic acid as starting materials, with 1,2-dichloroethane utilized as an extractant. Response surface analysis (RSM) was adopted to determine appropriate test design factors and levels using single-factor experiments as a basis, and a model was constructed by means of the Box–Behnken Design (BBD) central combination principle. …”

Hippocampal peroxidative damage in mice undergoing 2- or 4-mo moderate treadmill running. by Stefano Falone (301445)

“…<p>Assessment of hippocampal levels of thiobarbituric acid-reactive substances (TBARS) levels in mature CD-1 female mice undergoing a two- or four-month moderate and regular treadmill-based exercise program (E2 or E4, respectively); age-matched sedentary animals (S2, S4) were used as controls (n = 12 per group). TBARS levels were found to be increased by the sedentary aging (S4 vs S2). 2-mo exercise increased TBARS hippocampal concentrations (E2 vs S2), while 4-mo running decreased TBARS levels (E4 vs S4). …”

Transition Metal Substitution Effects on Metal-to-Polyoxometalate Charge Transfer by Elliot N. Glass (1648816)

“…A series of hetero-bimetallic transition metal-substituted polyoxometalates (TMSPs) were synthesized based on the Co^II-centered ligand [Co^IIW₁₁O₃₉]^10–. …”

Willin is an upstream regulator of EphrinB2 and EGFR. by Susana Moleirinho (400574)

Information S1 - Hypoxia Increases Gefitinib-Resistant Lung Cancer Stem Cells through the Activation of Insulin-Like Growth Factor 1 Receptor by Akiko Murakami (515972)

“…Treatment with YC-1 significantly decreased the number of HIF1α-positive cells in a dose-dependent manner. …”

Knock out VGLL4 in valvar endothelial cells leads to hypertrophy and compromised heart function on later stage. by Wei Yu (43094)

“…<p>(A) Gross front view of hearts from 9-month-old <i>Tie2</i>^<i>Cre+</i><i>;Vgll4</i>^<i>fl/-</i> and <i>Vgll4</i>^<i>fl/-</i> control hearts. …”

Larvicidal and macrofilaricidal efficacy of Closantel and Morantel against mosquito larvae and <i>Setaria digitata</i> nematodes by Megan Hall (506400)

“…While malaria incidence in India has decreased by nearly 50% in recent years, India holds 66% of the WHO SEA region’s malaria burden (World Malaria Report, 2023, Singh et al., 2024). …”

Microsatellite Instability and Mistmatch Repair protein analysis by Salvador J. Diaz-Cano (100072)

“…<p>Microsatellite Instability Testing in CRC and Interpretation</p> <p>Microsatellite instability is a type of mutation that occurs in microsatellite regions (short polymorphic DNA segments) by nucleotide addition (increased size) or nucleotide loss (decreased size) in the repeats. …”

The tombusvirus p33 replication protein binds to the yeast Scs2p VAP protein in the ER. by Daniel Barajas (255452)

“…<p>(A) The split ubiquitin assay was used to test binding between p33 and Scs2p in wt (NMY51) yeast. …”

Intracellular VBNC cells maintain phenotype and viability in BMDMs. by Raffael Júnio Araújo de Castro (3875755)

Functional validation of a miRNA-34a-TREM2–3’UTR interaction. by Surjyadipta Bhattacharjee (3181674)

“…<p><b>(A)</b> ribonucleotide sequence of the 299 nt TREM2-mRNA-3’-UTR is shown in the 5’-3’ direction; the 22 nt miRNA-34a-TREM2 3’UTR complementarity-interaction region is indicated by a black underline and the 8 nt TREM2-mRNA-3’-UTR seed sequence <b>5’-ACACUGCU-3’</b> is overlaid in yellow; a single arrowhead indicates the 5’ end of a poly A+ tail in the TREM2 mRNA (22 ‘A’ nt shown; the length of this poly A+ tail is variable); TREM2 mRNA sequence derived from NM_018965; TREM2 transcript is the major X1 variant (see also <a href="http://switchdb.switchgeargenomics.com/productinfo/id_801321/" target="_blank">http://switchdb.switchgeargenomics.com/productinfo/id_801321/</a>) (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a></b>); <b>(B)</b> TREM2-mRNA-3’UTR expression vector luciferase reporter assay (pLightSwitch-3’UTR; Cat#S801178; Switchgear Genomics, Palo Alto CA); in this vector, the entire 299 nucleotide TREM2 3’UTR was ligated into the unique Nhe1-Xho1 site; <b>(C)</b> control C8B4 murine microglial cells, 1 week in culture; phase contrast bright field microscopy 20x; C8B4 cells transfected with the TREM2-mRNA-3’UTR expression vector luciferase reporter were treated exogenously with miRNA-34a, a scrambled control miRNA-34a (miRNA-sc) or control miRNA-183; see references and text for further details [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref018" target="_blank">18</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref019" target="_blank">19</a>]; <b>(D)</b> compared to control, C8B4 cells transfected with a scrambled (<b>sc</b>) control pLightSwitch-3’UTR vector, the TREM2-mRNA-3‘UTR vector exhibited decreased luciferase signal to a mean of 0.16-fold of controls in the presence of miRNA-34a; this same vector exhibited no change in the presence of the control miRNA-34a-sc or miRNA-183; for each experiment (using different batches of MG cells) a control luciferase signal was generated and included separate appropriate controls with each analysis; in addition a control vector β-actin-3’UTR showed no significant effects on the relative luciferase signal yield after treatment with either miRNA-183 or miRNA-34a (data not shown); dashed horizontal line set to 1.0 for ease of comparison; N = 5; *<i>p</i><0.001 (ANOVA). …”

Reduced control model M4: Normative evidence accumulation. by Veith Weilnhammer (2909060)

“…History-congruent amounted to 50.73% ± 0.07% of trials (in blue). As in the full model, the no-oscillation model showed a significant bias toward perceptual history T(4.32×10³) = 9.94, <i>p</i> = 4.88×10⁻²³; upper panel). …”

Persistently increased percent predicted REE indicate prolonged hypermetabolism (A). by Marc G. Jeschke (165008)

“…<p>REE % predicted increases upon burn injury and decreased over time but remains significantly elevated up to two years post-injury. …”

Fig 3 - by Adrianne N. Edwards (5646344)

“…<p><b>RgaR-dependent gene expression is similarly decreased in both the <i>rgaS</i> and <i>rgaR</i> mutants.…”

The inhibition of the growth of MCF-7 and A549 cell lines in a dose-dependent manner by RSV. by Kuan-Yu Chen (58762)

“…After 12 days of incubation, the cells were stained with crystal violet and counted by phase-contrast microscopy. …”