CD86 and IL-12p70 Are Key Players for T Helper 1 Polarization and Natural Killer Cell Activation by Toll-Like Receptor-Induced Dendritic Cells by Felix S. Lichtenegger (139363)

“…Functionally, these signals translated into an increase in IFN-γ secreting Th1 cells and a decrease in regulatory T cells. T cell activation and polarization were dependent on IL-12p70 and CD86, but remarkably not on CD80 signaling. …”

Non-contributory pension programs and frailty of older adults: Evidence from Mexico by Emma Aguila (3461918)

“…We found the state pension led to a statistically significant decrease in the severity of frailty for women, but the federal pension was associated with an increase. …”

Hypocotyl properties under single versus multiple stress conditions in wildtype and <i>bin2-1</i> mutants. by Nils Kalbfuß (14248277)

“…Mean cell width significantly (P < 0.00001; not shown) decreased between light and dark and again between dark and darkW (H). …”

Chromatin immunoprecipitation (ChIP) using IgG isotype control, anti-Ezh2, and anti-H3K27me3 antibodies. by Falak Sher (151989)

“…Reduction in Ezh2 protein resulted in a decrease in the global level of H3K27 tri-methylation, an Ezh2-associated gene repression mark (17 kDa band). …”

Post-treatment controllers differ from HIV controllers in terms of HLA class I profile, frequency and quality of the CD8+ T cell response and activation levels of CD8+ T cells. by Asier Sáez-Cirión (3141075)

“…</b> The capacity of CD8+ T cells from VIRs (n = 22), ARTs (n = 14), HICs (n = 73) and PTCs (n = 14) to suppress the HIV-1 infection of autologous CD4+ T cells, as determined by the log-fold decrease in the level of secreted p24 (CD4 vs. …”

Steady state changes in leading edge activity sensors following modulator addition. by Brian P. Ziemba (327744)

“…Filled bars represent the fold change as the activity approaches a new steady state level approximately 5 min after modulator addition (t = 4.5 to 5.5 min (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196678#sec012" target="_blank">Methods</a>)). …”

Assessment of cell viability using quantification of lactate dehydrogenase (LDH) released by lesioned retinas treated with GJ blockers and openers. by Vera Paschon (306542)

“…We have tested two different concentrations of the pharmacological agents, i.e. 50 and 100 µM. We observed no changes in LDH release in retinal explants treated with (A) CBX 50 µM, (B) Quin 50 µM, (C) CBX+Quin 50 µM and (D) TMA 50 µM. …”

Identification of host factors required for HCV RNA replication or IRES-mediated translation. by Qisheng Li (522711)

“…Values relative to siNT in the heatmap are depicted in a continuum of blue (reduced infection - more than 50% decrease of the replicon activity after siRNA-mediated silencing; proviral) to red (enhanced infection - more than 50% increase of the replicon activity after siRNA-mediated silencing; antiviral). …”

Data_Sheet_1_Sex difference in circulating soluble form of ACE2 protein in moderate and severe COVID-19 and healthy controls.PDF by Josefina Robertson (14234798)

“…When comparing healthy controls [mean age 58.1 (SD 11.4) years] and patients with moderate COVID-19 [mean age 61.0 (SD 13.2) years] with patients in the ICU [mean age 63.6 (SD 11.6) years], we found that sACE2 concentration decreased (70% reduction) with disease severity in men (p = 0.002) but increased 3.7-fold with severity in women (p = 0.043), suggesting a sex-related difference in how COVID-19 severity is related to sACE2 concentration. …”

Putrescine binding analysis by fluorescence and far-UV CD spectroscopy. by Saurabh Pratap Singh (423493)

“…(C) Change in secondary structure, with increasing concentration of putrescine (0–200 µM) was monitored by molar ellipticity value at θ₂₂₂, curve showing change in secondary structure with increasing concentration of putrescine upto 50 µM. (D) Limited proteolysis with increasing concentration of putrescine (10–90 µM) also does not show effect on folding pattern of <i>L. donovani</i> ADL. …”

Lin28 increased the mammosphere formation rate and ALDH1 activity in breast cancer cells. by Yujie Liu (188278)

“…C. A soft-agar assay revealed that colony formation decreased in MDA-MB-231 cells after transfection with Lin28-siRNAs. …”

The effect of rottlerin on MACC1 expression. by Manisha Juneja (4059493)

“…MACC1 mRNA expression was analyzed by qRT-PCR. Samples with a 50% decrease in MACC1 mRNA levels are highlighted with black bars. …”

UV cones are efficiently ablated using our model of cell specific ablation. by Gordon F. Hagerman (3366290)

“…Auto-fluorescence is included for spatial reference (Green). Scale bars are 50 μm. <b>L.</b> UV cones were reduced in abundance four-fold after prodrug MTZ treatment (red bars) relative to vehicle control (DMSO; grey bars). …”

Independent qRT-PCR validation of genes that were differentially expressed in <i>Mfrp^rd6/Mfrp^rd6</i> mutants by array analysis. by Ramani Soundararajan (651915)

“…<p>The upregulated gene <i>Prss56</i> was evaluated at three different time points. (A) At P7, there was a 3.5-fold increase in <i>Prss56</i> transcript and it increased to 14-fold by P14, followed by a further 70-fold increase in <i>Prss56</i> transcript in <i>Mfrp^rd6/Mfrp^rd6</i> mutants at P21. …”

Antibody titers and cytokines production against SLA after infection in control and vaccinated mice groups. by Manuel Soto (111152)

“…In (A) ⁺⁺ (<i>P</i> < 0.01) and ** (<i>P</i> < 0.01) statistical decrease in anti-SLA response in the LiPABPs vaccinated group with respect saline or pcDNA3 control groups, respectively. …”

Supplementary Material for: Nomograms of Fetal Right Ventricular Fractional Area Change by 2D Echocardiography by Guirado L. (6174176)

“…In contrast, RV FAC showed a slight quadratic decrease throughout gestation (mean RV FAC ranged from 36% at 18 weeks of gestation [10–90th centiles: 25–47%, respectively] to 29% at 41 weeks [10–90th centiles: 18–40%, respectively]). …”

Experimental mutagenesis results. by Barry J. Grant (19471)

“…Mutant R326K shows a modest decrease in V_max for microtubule-activation, with a 3-fold higher K_m.…”

Modulation of endocannabinoid levels by R-flrubiprofen in microglial cells (EOC20) and tissue. by Philipp Bishay (248901)

“…<p><b>a</b> Endocannabinoids in EOC20 at baseline and after stimulation with LPS (1 mg/ml) with or without treatment with R-flurbiprofen (10 µM); n = 6 experiments per group. …”

The formation of surface-induced intermediate precedes the loss of native subunit conformation. by Jian-Min Lv (5324774)

“…Lowering pH had little effect on the process of pentamer disassembly (changes of 3H12 and 8D8 showed the same tendency as above) but resulted in continuous structure remodeling of the structure of the dissociated subunit (8C10 rise and 1D6 decrease). Data were obtained from at least three independent experiments and represented as mean ± SEM. …”

Image_3_YAP/TAZ: Key Players for Rheumatoid Arthritis Severity by Driving Fibroblast Like Synoviocytes Phenotype and Fibro-Inflammatory Response.tif by Robin Caire (11816078)

“…Inhibiting YAP/TAZ transcriptional activity with VP restored a common phenotype in RA FLS with a decrease in apoptosis resistance, proliferation, invasion, and inflammatory response. …”