M-cells are depleted with αRANKL antibody treatment. by Aimee L. Cunningham (650204)

“…Representative images showing normal morphology of intact (ratIg-treated) PP were taken using combined phase contrast and fluorescence at 40x (a), or labeled M cells (480 in rat Ig-treated PP on day 8, 65 M-cells in αRANKL-treated PP on day 8, or 476 in αRANKL-treated PP on day 16) all with fluorescence alone at 100x (b-d). …”

Turnover of 4MUG substrate reads out GBA1 activity in human brain lysates in the presence of sodium taurocholate. by Zdenek Berger (440685)

“…When converted to specific activity, similar values are obtained when using 10–60 μg of human cortex lysates, as expected. A representative experiment is shown. C) Turnover of 4MUG substrate in lysates from human cortex is mostly due to GBA1 activity when using sodium taurocholate (NaTc). 1 mM CBE and 1 μM isofagomine (Iso) decrease the signal by ~ 90% in the presence of sodium taurocholate whereas substantially smaller reduction (~ 30%) is seen without sodium taurocholate in the assay buffer. 10 μg of protein lysate was used. …”

Gas-Phase Surface Engineering of Polystyrene Beads Used to Challenge Automated Particle Inspection Systems by Vickie Labonté (2861663)

“…Following treatment, newly grafted C–OH, C–O–C, CO, and COOH functional groups on the bead’s surface are observed by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy, leading to an increase in the surface energy from 31 ± 1 to 65 ± 2 mJ/m² and a corresponding zeta potential decrease from −38 to −61 mV. …”

The effect of 5-LOX on TRPM7-mediated cell rounding and TRPM7 current density. by Hsiang-Chin Chen (247146)

“…The asterisk indicates a significant difference in cell rounding between 5-LOX knockdown (dsiLOX5-1 & dsiLOX5-2) and control cells (dsiCT), using Student's <i>t</i> test (<i>p</i><0.05) (D) Knockdown of 5-LOX did not appreciably decrease TRPM7's current density at +100 mV in 293-TRPM7 expressing cells transfected with dsiLOX5-2 compared to cells transfected with the control (dsiCT). …”

Percent binding of BK VP1 serotype II-IV to HEK293TT cells in the presence or absence of antibodies. by Marcel Weber (581069)

“…<p>Median fluorescent intensity of plots shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1013122#ppat.1013122.s001" target="_blank">S1 Fig</a> were extracted using FlowJo 10.7.1 and normalized to 100% VP1 binding (VP1 II, III or IV binding to HEK293TT cells in the absence of antibodies) and 0% VP1 binding (HEK293TT cells with detection antibody only). …”

Celastrol Promotes Weight Loss in Diet-Induced Obesity by Inhibiting the Protein Tyrosine Phosphatases PTP1B and TCPTP in the Hypothalamus by Eleni Kyriakou (2063644)

“…Celastrol-administered mice at 100 μg/kg decrease food consumption and body weight via a leptin-dependent mechanism, yet its molecular targets in this pathway remain elusive. …”

Ileitis-induced dysbiosis is associated with increased bacteria and clonal proliferation of adherent invasive <i>E. coli</i> (AIEC). by Melanie Craven (148451)

“…Mild pathology (T4, G14) induces a decrease. (b) Moderate to severe ileitis (T8, HDI) induces <i>E. coli</i> proliferation (<i>E. coli uidA</i> quantification by real-time PCR). …”

Relative Cx43 expression in C6 and F98 cells detected by western blot and RT- PCR analyses. by Zahra Moinfar (2504494)

“…<p>a) F98 cells showed a significant increase and b) C6 cells showed a significant decrease of Cx43 protein expression after treatment with 10 nM or 100 nM E2. c) Transfection of C6 cells with Cx43-cDNA neutralized these effects. d) RT-PCR showed an opposite effect of E2 on Cx43 gene expression in C6 when compared to F98 cells. e) A representative WB showing the increased Cx43 protein expression in F98 cells and the decreased Cx43 expression in C6 cells, after E2 treatment. …”

Age-Dependent Impacts of Lighting Conditions on Contrast Sensitivity and Subjective Visual Perception Focusing on Pupil Size and Crystalline Lens Transmittance by Taisuke Eto (17892397)

“…The six lighting conditions used were a combination of three illuminances (100, 300, and 750 lx) and two CCTs (3000 and 6000 K). …”

DataSheet1_Beneficial effect of Xuebijing against Pseudomonas aeruginosa infection in Caenorhabditis elegans.doc by Le Zhang (88249)

“…Although Xuebijing did not show obvious antibacterial activity, Xuebijing (100%) treatment could inhibit the Pseudomonas biofilm formation and decrease the expression of virulence genes (lasA, lasB, rhlA, rhlC, phzA, phzM, phzH, and phzS) and quorum sensing (QS)-related genes (lasI, lasR, rhlI, rhlR, pqsA, and pqsR). …”

Flagellar size regulation. by Deb Sankar Banerjee (12890259)

“…<p>(A) Flagellum assembly in <i>C. reinhardtii</i> is regulated by IFT particles that incorporate tubulin dimers at the flagellum tip. …”

Selective VCP mediated proteostasis-inhibition controls the growth of NSCLC colonies. by Kyla Walworth (3150936)

“…Fresh media with control-PBS-vehicle, empty-dendrimer (DDN), DBeQ (50μM, positive control) or DBeQ-encapsulated dendrimer (DDN^DBeQ, 50μM) was added to the top of the cells suspended in agarose. The plate was kept in a 37°C incubator until colonies were visible and pictures were captured using a Nikon Eclipse TS100 inverted light microscope at 10x phase objective magnification. …”

DataSheet1_Combined toxicity of multiwall carbon nanotubes and cadmium on rice (Oryza sativa L.) growth in soil.PDF by Jinghua Long (17981163)

“…The single and combined effects of multiwall carbon nanotubes (MWCNTs) and cadmium (Cd) on rice (Oryza sativa L.) growth were examined in this study through a 4 months pot experiment in 2022. Rice plants were exposed to different concentrations of MWCNTs (100 and 500 mg kg⁻¹) in the presence of 5.0 mg kg⁻¹ Cd stress. …”

Gas-Phase Surface Engineering of Polystyrene Beads Used to Challenge Automated Particle Inspection Systems by Vickie Labonté (2861663)

“…Following treatment, newly grafted C–OH, C–O–C, CO, and COOH functional groups on the bead’s surface are observed by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy, leading to an increase in the surface energy from 31 ± 1 to 65 ± 2 mJ/m² and a corresponding zeta potential decrease from −38 to −61 mV. …”

Mitochondrial membrane potential and ATP levels were reduced more by combination therapy than by single treatments of SLCP or BBR in U-87MG and U-251MG cells. by Panchanan Maiti (1459852)

“…<b>A</b>: Representative images showed a decrease in the number of mitochondria in SLCP- and BBR-treated cells, with combination treatments showing larger decreases. …”

Natural Variants of <i>C. elegans</i> Demonstrate Defects in Both Sperm Function and Oogenesis at Elevated Temperatures by Lisa N. Petrella (656347)

“…In contrast, all isolates show a progressive decrease in brood size as temperature increases from 20°C to 26°C, followed by a brood size near zero at 27°C. …”

Fig 8 - by William J. Dower (17277207)

“…<p><b>Test compounds induce decrease in cell surface IL-7R</b>α. Rested human CD8+ T-cells were treated with saturating concentrations of the test agonists (10 nM IL-7; 1 μM MDK1472 or MDK-703) or the negative control compounds (untreated, 1 μM MDK1169, or MDK-202) for 20 minutes on ice, then incubated at (A) 37°C or (B) 0°C for varying times to monitor uptake of IL-7Rα from the cell surface. …”

Yield, quality and drought sensitivity of tomato to water deficit during different growth stages by Jintao Cui (7397102)

“…Compared to the control treatment, drought stress at stages II and III caused a decrease in yield of 13 % and 26 %, respectively. …”

Ordered effector interplay revealed by <i>trans</i> BENEFIT screening. by Robert J. Cain (272233)

“…Table shows differences in invasion rates (%) after correction. Shading denotes a significant increase (green), significant decrease (red) or no significant change (grey) in invasion (Mann Whitney U p<0.05).…”

Data Sheet 1_Effects of karst environmental stresses on seed germination and seedling growth of alfalfa (Medicago sativa L.).pdf by Zijun Zhou (3602705)

“…Under moderate calcium stress (40 mM), only a slight decrease in tissue water content was observed. …”