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    Fig 7 - by Finn Egil Skjeldestad (7821716)

    Published 2024
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    Design, expression and functional characterization of a thermostable xylanase from <i>Trichoderma reesei</i> by Jun He (46556)

    Published 2019
    “…<i>reesei</i> xylanase (Xyn2), and novel flexible regions of the enzyme were identified based on B-factor, a molecular dynamics (MD) parameter. To improve thermostability of the Xyn2, disulfide bonds were introduced into the unstable flexible region by using site-directed mutagenesis and two recombinant xylanases, XM1 (Xyn2<sup>Cys12-52</sup>) and XM2 (Xyn2<sup>Cys59-149</sup>) were successfully expressed in <i>Pichia pastoris</i>. …”