Data_Sheet_1_Tfam Knockdown Results in Reduction of mtDNA Copy Number, OXPHOS Deficiency and Abnormalities in Zebrafish Embryos.PDF by Auke B. C. Otten (8965244)

“…Morphant embryos displayed abnormal development of the eye, brain, heart, and muscle, as well as a 50 ± 22% decrease in ATP production. Transcriptome analysis revealed a decrease in protein-encoding transcripts from the heavy strand of the mtDNA, and down-regulation of genes involved in haem production and the metabolism of metabolites, which appear to trigger increased rRNA and tRNA synthesis in the nucleoli. …”

Data_Sheet_3_Tfam Knockdown Results in Reduction of mtDNA Copy Number, OXPHOS Deficiency and Abnormalities in Zebrafish Embryos.PDF by Auke B. C. Otten (8965244)

“…Morphant embryos displayed abnormal development of the eye, brain, heart, and muscle, as well as a 50 ± 22% decrease in ATP production. Transcriptome analysis revealed a decrease in protein-encoding transcripts from the heavy strand of the mtDNA, and down-regulation of genes involved in haem production and the metabolism of metabolites, which appear to trigger increased rRNA and tRNA synthesis in the nucleoli. …”

Data_Sheet_2_Tfam Knockdown Results in Reduction of mtDNA Copy Number, OXPHOS Deficiency and Abnormalities in Zebrafish Embryos.pdf by Auke B. C. Otten (8965244)

“…Morphant embryos displayed abnormal development of the eye, brain, heart, and muscle, as well as a 50 ± 22% decrease in ATP production. Transcriptome analysis revealed a decrease in protein-encoding transcripts from the heavy strand of the mtDNA, and down-regulation of genes involved in haem production and the metabolism of metabolites, which appear to trigger increased rRNA and tRNA synthesis in the nucleoli. …”

Data_Sheet_4_Tfam Knockdown Results in Reduction of mtDNA Copy Number, OXPHOS Deficiency and Abnormalities in Zebrafish Embryos.PDF by Auke B. C. Otten (8965244)

“…Morphant embryos displayed abnormal development of the eye, brain, heart, and muscle, as well as a 50 ± 22% decrease in ATP production. Transcriptome analysis revealed a decrease in protein-encoding transcripts from the heavy strand of the mtDNA, and down-regulation of genes involved in haem production and the metabolism of metabolites, which appear to trigger increased rRNA and tRNA synthesis in the nucleoli. …”

Loss of intestinal epithelial Shh signaling deregulates intestinal crypt epithelial proliferation. by Jessica Gagné-Sansfaçon (571087)

“…Proliferation assays were performed by PCNA immunostaining (G and H, green labeling), with Evans blue serving as counterstain (red staining). Mutants displayed a decrease in cell proliferation as shown by a decrease in the number of PCNA-labeled proliferating cells (H) when compared to controls (G). …”

Displacement of site marker ligands bound to HSA in presence of increasing PS concentrations. by Shevin R. Feroz (468333)

“…Spectra marked as ‘a’, ‘b’, ‘c’ and ‘d’ refer to 10 µM BR, 10 µM HSA, 50 µM PS and PS–HSA (5∶1) complex, respectively. …”

Treatment with either THUC or THUB reduces the formation of (B27-HC)₂. by Hui-Chun Yu (470632)

“…<p>After treatment with each protein for the indicated time point, membrane proteins were extracted. 50 μg of extract was resolved by non-reducing SDS-PAGE (10%) and immunoblotted with a BH2 monoclonal antibody and anti-transferrin (Tf) receptor antibody. …”

Effect of perifusion flow rate on islet oxygen. by Wanyu Chen (175127)

“…<p>Encapsulated islets were perifused at 180 µL/min in KRB containing 3 mM glucose. At time = 15 minutes, the flow rate was changed to 50 µL/min, and at time = 70 it was decreased back to 180 µL/min. …”

Data_Sheet_1_Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta.DOCX by Sabrina Saibi (3610550)

“…In 12% biosolids in the slurry, the oxidative potential measured by the ABTS assay (O_ABTS) reached 5,000 mM of O_ABTS in the presence of PhACs, and 2,500 mM of O_ABTS without PhACs, as compared to 1,200 mM of O_ABTS in control culture. …”

Table_1_Soil carbon, nitrogen, and phosphorus stoichiometry and its influencing factors in Chinese fir plantations across subtropical China.doc by Ran Tong (11517648)

“…The results showed that the range of SOC: TN (C:N), SOC: TP (C:P), and TN: TP (N:P) ratios were 7.32–18.27, 20.15–230.48, and 2.11–15.05 with a mean value of 13.22, 83.50, and 6.05, respectively. …”

Table_1_Soil carbon, nitrogen, and phosphorus stoichiometry and its influencing factors in Chinese fir plantations across subtropical China.doc by Ran Tong (11517648)

“…The results showed that the range of SOC: TN (C:N), SOC: TP (C:P), and TN: TP (N:P) ratios were 7.32–18.27, 20.15–230.48, and 2.11–15.05 with a mean value of 13.22, 83.50, and 6.05, respectively. …”

Shh signaling is required for proper production of goblet cell secretory products and Paneth cell maturation. by Jessica Gagné-Sansfaçon (571087)

“…Immunostaining with an anti-lysozyme antibody showed a decrease in positively-labeled lysozyme cells (green staining) in mutant mice (E) compared to control littermates (D). …”

table_1_In Utero and Postnatal Propylthiouracil-Induced Mild Hypothyroidism Impairs Maternal Behavior in Mice.DOCX by Miski Aghnia Khairinisa (5213834)

“…As expected, locomotor and cognitive functions in these mice were disrupted in a PTU dose-dependent manner. On postpartum day 2, dams who had been exposed 50 ppm PTU during in utero and postnatal periods displayed a significantly longer time identifying the first pup and returning all three pups back to the nest, less time nursing, and decreased licking behavior. …”

table_2_In Utero and Postnatal Propylthiouracil-Induced Mild Hypothyroidism Impairs Maternal Behavior in Mice.DOCX by Miski Aghnia Khairinisa (5213834)

“…As expected, locomotor and cognitive functions in these mice were disrupted in a PTU dose-dependent manner. On postpartum day 2, dams who had been exposed 50 ppm PTU during in utero and postnatal periods displayed a significantly longer time identifying the first pup and returning all three pups back to the nest, less time nursing, and decreased licking behavior. …”

image_1_In Utero and Postnatal Propylthiouracil-Induced Mild Hypothyroidism Impairs Maternal Behavior in Mice.EPS by Miski Aghnia Khairinisa (5213834)

“…As expected, locomotor and cognitive functions in these mice were disrupted in a PTU dose-dependent manner. On postpartum day 2, dams who had been exposed 50 ppm PTU during in utero and postnatal periods displayed a significantly longer time identifying the first pup and returning all three pups back to the nest, less time nursing, and decreased licking behavior. …”

“Deep” Sequencing Accuracy and Reproducibility Using Roche/454 Technology for Inferring Co-Receptor Usage in HIV-1 by David J. H. F. Knapp (444976)

Effect of fucoidan on the expression GRP78 and ERp29. by Shaohua Chen (114324)

“…<p>Cells at 70–80% confluence were treated with fucoidan at the indicated concentrations for 3 days and both the attached and suspended cells were harvested for protein expression analysis. (A) The expression of GRP78, but not ERp29, was inhibited by fucoidan in MDA-MB-231 cells; (B) The expression of ERp29 was significantly attenuated by fucoidan in HCT116 cells, while GRP78 was only slightly decreased (∼1.5-fold) in cells treated with fucoidan at >50 µg/ml. …”

Timecourse of Cu^II(gtsm)-mediated ERK and JNK phosphorylation and the effect of specific kinase inhibition on neurite elongation in NGF-treated PC12 cells. by Laura Bica (227741)

“…<p>(<b>A & B</b>) Cu^II(gtsm) (18 hr, 50 nM) decreased ERK and JNK activation from the 8 hr timepoint. …”

ToxCast™Phase I chemical activity across cytotoxicity and differentiation ES cell assay endpoints. by Kelly J. Chandler (218453)

“…Unsupervised, two-dimensional hierarchical clustering of 320 ToxCast chemicals across four ES cell assay endpoints (cytotoxicity increase, cytotoxicity decrease, differentiation increase, differentiation decrease) and 4 doses each using Euclidean distance for measure and Ward's method for linkage analysis (A). …”

The exocyst is required for cell cycle progression in trypanosomes. by Cordula M. Boehm (3689848)

“…Sec15::RNAi cells have a ∼50% decrease to Sec15::HA 48h post induction, whilst expression of Exo99 remains stable, and <i>vice versa</i>. …”