Effects of ligand stimulation and targeted therapeutics on IGF1R and IR expression and phosphorylation. by Jurriaan Brouwer-Visser (578686)

“…Cells were starved overnight, incubated with 1 µM NVP-AEW541 or 10 µg/ml IMC-A12 for 2 hours, stimulated with 50 ng/ml IGF2 or 50 nM insulin for 10 minutes, lysed, and levels of phosphorylated IGF1R and IR determined by ELISA. …”

Changes to chromatin state with PRMT5 inhibition. by Shelby L. Sloan (10803222)

“…(D) Immunoblot analysis indicates a time dependent decrease in MYC protein after treatment with C220 in 17–71 (100 nM) and CLBL-1 (20 nM). …”

Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors-2 by Mark D Andrake (95479)

“…Quantitative analysis of nuclear import of various GST fusion proteins with (+ comp) and without (no comp) competitor. More than 100 cells were counted for each experimental condition and the percentage of cells that had a mostly nuclear staining for the fusion protein was calculated. …”

Somatic Focus manipulated with incentivized discrimination tasks. by Natalie E. Johnston (318841)

“…Payoffs were determined from a subset of trials (20). The maximum additional reward compensation a participant could earn was $21 and the minimum was $0. …”

Effect of application of monomethyl fumarate in organotypic slice cultures of hippocampus after 30 min of OGD. by Oliver Althaus (19346776)

“…In order to ascertain the percentage of cell death, the PI signal was normalized to the DAPI signal and expressed as a percentage. The slice culture group that followed OGD with no drug treatment was set at 100% for the proportion of identified cell death. …”

Measurement of the impedance of the HBEC monolayers during coculture with IRBC. by Ronan Jambou (80268)

“…A) show decrease of impedance when HBEC monolayer is co-cultured with IRBC(3Ci) or IRBC(3Ci)+NRBC (vol/vol), but not when co-cultured with normal RBC). …”

Alterations in tone and FM sweeps evoked activity during repetitive stress. by Ghattas Bisharat (20706928)

“…There was a decrease in activity during repetitive stress (mean ± SE, 2-way ANOVA condition F = 13.9, <i>p</i> = 2.03 × 10⁻⁰⁴, condition: intensity interaction F = 4.3, <i>p</i> = 0.01, nested ANOVA (mouse nested within session) F = 2.1, <i>p</i> = 0.001 condition: intensity interaction F = 2.1, <i>p</i> = 0.001), especially at moderate sound intensities (post hoc 50 dB <i>p</i> = 2.36 × 10⁻⁰⁴, but not at lower 30 dB <i>p</i> = 1 or higher intensities 70 dB <i>p</i> = 1 Bonferroni corrected). …”

Oxidative stress in INS1E and alphaTC1 cells in 3D aggregates. by Mireille M. J. P. E. Sthijns (12245491)

“…B) Seeding INS1E and alphaTC1 cells in the ratios 100:0 and 50:50 in 3D aggregates exposed to 500 μM H₂O₂ decreased the percentage of oxidative stress–positive INS1E cells. …”

Quantitative determination of loss of MMP in SH-SY5Y cells in response to Grx1 knockdown. by Uzma Saeed (49670)

“…Cells were treated with vehicle after transfecting them with empty vector, or with vehicle, α-lipoic acid (100 µM) or cyclosporine A (10 µM), 6 hr post transfection with shRNA to Grx1 and MMP was measured using TMRM as the indicator dye. …”

Data_Sheet_1_Trends and Outcomes of Aortic Valve Replacement in Patients With Diabetes in the US.docx by Sarah Khan (709687)

“…There was a significant temporal decrease in mortality, acute renal failure (ARF), and stroke. …”

Sequence gaps exclusive to both Archaea and Microsporidia 16S rRNAs. by Chaitanya Muralidhara (223748)

“…(<i>a</i>) The 100 gaps conserved in both the Archaea and Microsporidia map to the entire substructures I–III in the secondary structure model of the bacterium <i>E. coli</i> <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018768#pone.0018768-Cannone1" target="_blank">[14]</a>. …”

PDBu (1 µM) increases the amplitude of outward K⁺ currents. by Sébastien Hayoz (5656207)

“…<p>Panel A: K⁺ currents elicited by linear voltage ramps varying from −100 to 100 mV. …”

The complementary chimera IIS4I shifts the gating of Ca_V1.2 channels towards low-voltage behavior. by Ana Laura Sanchez-Sandoval (4885423)

“…For clarity purposes only the last 50 ms of the prepulses are shown. Note that chimeric channels availability decreases proportionally as the <i>V</i>_m become more positive, similar to the behavior of Ca_V3.3-WT channels (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0193490#pone.0193490.g004" target="_blank">Fig 4</a>). …”

Acute reduction of Ezh2 induces apoptosis in NSCs. by Falak Sher (151989)

“…Immunostaining of dissociated neurospheres in proliferation medium shows a decrease in proliferation and increase in cell death after using Ezh2-shRNA compared to the control (nc-shRNA). …”

Robustness to noise. by Martin Boerlin (355887)

“…Results are reported for percentual decreases in the signal-to-noise ratio, SNR = mean(input)/std(input), of 0% (black), 20% (blue), 50% (red) and 100% (green). …”

Sholl analysis and spine density analysis in mouse hippocampal neurons. by Qiang-qiang Xia (8060987)

“…WT N = 50/10 cells/mice; HET N = 50/10). *P<0.05, **P<0.01, ***P<0.001, unpaired student’s t-test; graphs depict mean ± SEM. …”

Long-term trends of potential years of life lost due to main causes of death in the Slovak population, 2004-2013 by Martin Samohyl (10388448)

“…The PYLL rate is age-standardized and expressed as a sum of all deaths per 100,000. In the period 2004-2013, the highest mean PYLL rates were observed in neoplasms in the whole population (2,103 per 100,000), as well as in females (2,088 per 100,000), with a permanent high significant increase of AAPC of PYLL in both genders. …”

HuR specifically represses translation through the IGF-IR 5′-UTR in a concentration-dependent manner by Zheng Meng (9293)

“…The IGFIR(1–1038)-FLuc RNA (5 ng) was incubated with increasing amounts of transcribed mRNA encoding HuR (5–50 ng) in an translation reaction (with RRL). …”

Cochlear hair cells are reduced in <i>Fgfr1</i> mutants. by Kazuya Ono (513633)

“…Cochlear length is decreased by approximately 40–50% in all <i>Fgfr1</i> mutants except <i>Fgfr1^Y766F/Y766F</i>. …”

The time-dependent currents in <i>Mytilus galloprovincialis</i> hemocytes are modulated by the osmotic pressure. by Monica Bregante (3391766)

“…D) The hyperosmotic bath solution determines a decrease of the steady-state current elicited by V = -100 mV (left bar) without altering the capacitance of the cells (right bar). …”