Data_Sheet_2_Exploring the Potential of CRISPR-Cas9 Under Challenging Conditions: Facing High-Copy Plasmids and Counteracting Beta-Lactam Resistance in Clinical Strains of Enteroba... by Thaysa Leite Tagliaferri (6371453)

“…One innovative approach is the CRISPR-Cas9 system which has recently been used for plasmid curing in defined strains of Escherichia coli. Here we exploited this system further under challenging conditions: by targeting the bla_TEM–₁ AMR gene located on a high-copy plasmid (i.e., 100–300 copies/cell) and by directly tackling bla_TEM–₁-positive clinical isolates. …”

DataSheet_1_Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells.docx by Kyung Youn Lee (12794388)

“…<p>Although birds have been used historically as a model animal for immunological research, resulting in remarkable achievements, immune cell development in birds themselves has yet to be fully elucidated. In this study, we firstly generated an immunodeficient chicken model using a CRISPR/Cas9-mediated recombination activating gene 1 (RAG1) knockout, to investigate avian-specific immune cell development. …”

Table 9_Interactions between the intestinal microbiome and host genes in regulating vibriosis resistance in Cynoglossus semilaevis.xlsx by Weiwei Zheng (140828)

“…</p>Methods<p>In this study, we investigated the changes in intestinal histopathology, intestinal microbiome and host gene expression in resistant and susceptible individuals at 7 days post infection with Vibrio harveyi, and identified the host gene-microbe correlations. …”

Data_Sheet_1_Influence of 37 Years of Nitrogen and Phosphorus Fertilization on Composition of Rhizosphere Arbuscular Mycorrhizal Fungi Communities in Black Soil of Northeast China.... by Qingfeng Wang (1356576)

“…In this context, a long-term fertilizer experiment (37 years) was set up in the black soil of northeast China. We examined interaction impacts of elevated fertilizer and host selection processes on arbuscular mycorrhizal fungi (AMF) communities in wheat rhizosphere soil using the Illumina MiSeq platform. …”

Contrasting roles of cytochrome P450s in amitraz and chlorfenapyr resistance in the crop pest <i>Tetranychus urticae</i> by Thomas Van Leeuwen (13004790)

Supplementary Material for: A Comparison of Serial Position Effects in Patients with Mild Cognitive Impairment due to Parkinson’s Disease or to Alzheimer’s Disease by Meyer A. (3224046)

DataSheet_4_Hypothyroidism has a protective causal association with hepatocellular carcinoma: A two-sample Mendelian randomization study.pdf by Likui Lu (6631655)

“…Objective<p>Observational studies suggest an association between hypothyroidism and the risk of hepatocellular carcinoma (HCC), but the causality and direction of these effects are still inconclusive. We aim to test whether hypothyroidism is causally associated with the risk of HCC by using Mendelian randomization (MR) analysis.…”

Table_3_Novel Genetic Variations in Acute Myeloid Leukemia in Pakistani Population.XLSX by Saba Shahid (9013928)

“…Notably, two recurrent somatic non-synonymous mutations, i.e., STAG2 (causing p.L526F) and BCORL1 (p.A400V), were observed in three unrelated cases each. …”

Table_1_100% Fruit Juice and Dental Health: A Systematic Review of the Literature.docx by DeAnn Liska (6948188)

“…</p><p>Results: Eight publications representing five independent prospective cohort studies and nine publications on nine RCTs were included. …”

Table_1_The Long-Term Effect of Biochar on Soil Microbial Abundance, Activity and Community Structure Is Overwritten by Land Management.XLSX by Brieuc Hardy (6628931)

“…Nevertheless, the long-term evolution of these effects is unknown and of critical importance because biochar persists in soil for centuries. We selected nine charcoal kiln sites (CKS) from forests (four sites) and croplands (five sites) and determined the microbial properties of their topsoil, largely enriched with charcoal for >150 years. …”

Image_2_The IAG-Switch and Further Transcriptomic Insights Into Sexual Differentiation of a Protandric Shrimp.TIF by Tom Levy (4708123)

“…The Northern spot shrimp, Pandalus platyceros, is a protandric hermaphrodite species, native to the North Pacific Ocean. We collected four stages of spot shrimp in Southeast Alaska including: juveniles, adult males, transitionals, and adult females. …”

Supplementary Material for: Comparison of Lung, Lobe, and Airway Volumes between Supine and Upright Computed Tomography and Their Correlation with Pulmonary Function Test in Patien... by Yamada Y. (3128277)

TBI is ESKD. by Belinda L. Baines (18446381)

“…Until recently there has been little evaluation of the impact of haemodialysis in limb and systemic perfusion on these values. We aimed to determine if the values of TBI would change during and after dialysis compared to pre-dialysis assessments.…”

Table_2_A Comparison of Forensic Age Prediction Models Using Data From Four DNA Methylation Technologies.XLSX by A. Freire-Aradas (9255545)

Table_1_Associations Between Sleep Quality and Health Span: A Prospective Cohort Study Based on 328,850 UK Biobank Participants.doc by Muhammed Lamin Sambou (10971984)

“…The hazard ratio (HR) and 95% confidence intervals (CIs) were calculated by multivariate-adjusted Cox proportional hazards model. Moreover, we calculated population attributable risk percentage (PAR%) to reflect the public health significance of healthy sleep quality.…”

Supplementary Material for: Determination of Cytokines and Oxidative Stress Biomarkers in Cognitive Impairment Induced by Methylmalonic Acidemia by Li Q. (2850350)

Image_2_Hypoxia Routes Tryptophan Homeostasis Towards Increased Tryptamine Production.jpeg by Soumya R. Mohapatra (8081411)

DataSheet2_Eocene-Oligocene southwest Pacific Ocean paleoceanography new insights from foraminifera chemistry (DSDP site 277, Campbell Plateau).XLSX by F. Hodel (14131221)

Data Sheet 1_Global, regional, and national burden and risk factors of ischemic heart disease, 1990–2021: an analysis of the global burden of disease study.pdf by Quankai Cheng (21177656)

“…Potential differences in burden and risk factors based on age, sex, 21 GBD geographic regions, five social development index (SDI) regions, and 204 countries are highlighted.…”

Table 6_Antifungal mechanism and transcriptome analysis of Bacillomycin D-C16 against Fusarium oxysporum.xlsx by Fuxing Lin (7418234)

“…Biochemical assays confirmed these observations: Reduced activities of mitochondrial enzymes [malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), pyruvate dehydrogenase (PDH), and complexes I–V], decreased mitochondrial membrane potential, and diminished ATP content collectively indicated mitochondrial dysfunction. …”