Competitive Regulation of E-Cadherin JuxtaMembrane Domain Degradation by p120-Catenin Binding and Hakai-Mediated Ubiquitination by Andrea Hartsock (160855)

“…We found WT-JMD was ubiquitinated, and arginine substitution of lysines at position 5 (K5R) and 83 (K83R) resulted in the stable accumulation of mutant JMD at mitochondria. p120-Catenin did not localize, or bind to WT-JMD even upon proteasome inhibition, whereas the K5,83R-JMD mutant bound and localized p120-catenin to mitochondria. …”

Tau knockout prevents sensitivity to Kainic acid–mediated stress in AICD-Tg mice. by Kaushik Ghosal (244754)

“…Quantification of NPY immunoreactivity normalized to WT animals. *p<0.05, **p<0.01, ***p<0.001, one way ANOVA (all data expressed as Mean ± SEM). n = 5 for all groups (D-G). …”

Accelerated recovery from mechanical and thermal hyperalgesia following nerve injury in EphB1 KO mice. by Vincent Cibert-Goton (288406)

“…This reduction in paw withdrawal latency was significantly smaller in EphB1 KO mice at all time points, except 5 days after injury. WT mice also developed mechanical allodynia with a marked decrease in stimulus intensity required to trigger a lifting response of the injured paw as compared to baseline. …”

Silencing in GFP-expressing THP-1 cells. (A) by Breanne Landry (138815)

“…(<b>B</b>) Silencing was assessed after 3 days of siRNA treatment (50 nM) and expressed as decrease in mean GFP fluorescence or decrease in GFP-positive cells. …”

PAR-2 deficiency exacerbates the body temperature during IgE-dependent and IgE-independent anaphylaxis. by Maho Nakazawa (18405297)

“…<p>(A) Decrease in body temperature during IgE-dependent anaphylaxis in WT and PAR-2 KO mice (n = 5 each). …”

Snail1 loss altered cytoskeletal organization and gene expressions in RMG1 cells. by Misako Haraguchi (768459)

“…Scale bar indicates 50 μm. WT cells exhibited cobblestone-like morphologies, whereas most of the Snail1 KO1 cells exhibit a more rounded morphology. …”

Basal ECD mutations have the greatest influence on activation. by Ákos Nemecz (356830)

“…<p>Stick representation of the evaluated Asp, Glu, and His residues are labeled and color coded based upon effect, with residues in red producing significant decrease in pH₅₀ as a single mutation, in pink a significant decrease in combination, in yellow an insignificant or weak effect, and in blue a significant increase in pH₅₀. …”

Preventing age-related degeneration of proteoglycans in articular cartilage by CCN2 overexpression. by Shinsuke Itoh (125939)

“…<p>(A) Toluidine blue staining of frontal sections of medial portion of TG (a, c, e) and WT (b, d, f) littermate knee joints from 5- (a and b), 12- (c and d), and 24- (e and f) month-old male mice. …”

Additional file 7: of Deletion of Nlrp3 protects from inflammation-induced skeletal muscle atrophy by Nora Huang (3661855)

“…Twelve- to 16-week-old male Nlrp3 KO and WT mice were subjected to CLP or sham surgery. qRT-PCR analysis of myosin heavy chain (Myh) 2, Myh4, and Myh7 expression in tibialis anterior (TA) muscles of sham (n = 5) and CLP (n = 5) mice at 96 h after surgery as indicated (WT sham: n = 5; WT CLP: n = 9; Nlrp3 KO sham n = 5; Nlrp3 KO: CLP n = 6). mRNA expression was normalized to Gapdh. …”

Synthesis of flexible polyurethane foams by the partial substitution of polyol by steatite by Plínio César de Carvalho Pinto (5834999)

“…There is not a significant change in the chemical composition of the foams, due to substitution of 4.5 wt. % polyol by steatite. However, there was a decrease in cell size and greater interaction between the hard segments of the composite.…”

cKO embryos display a variety of defects in the yolk sac mesoderm. by Siyeon Rhee (390224)

“…C, F) Section immunofluorescence of WT (C) and cKO (F) 9.5 <i>dpc</i> yolk sacs demonstrates loss of αSMA in the cKO. …”

Mutations at p53 binding sites augment PIK3CA promoter activity. by Snehal M. Gaikwad (279479)

“…These mutant promoters showed a gradual augment of PIK3CA expression compared to WT-PFT with MPFT 3 showing a maximum of 2.5-fold increase (*Fig. 5b). …”

Image_6_Function of the fliK Gene in Pseudomonas plecoglossicida Pathogenicity and Epinephelus coioides’ Immune Response.pdf by Zixu Liu (9204374)

“…The shRNA-406 mutant with the maximum silencing efficiency (97.5%) was chosen for further study. Compared with the wild-type (WT) P. plecoglossicida strain, silencing fliK in the fliK-RNA interference (RNAi) strain resulted in a significant decrease in growth, motility, chemotaxis, adhesion, and biofilm formation in P. plecoglossicida. …”

Table_1_Function of the fliK Gene in Pseudomonas plecoglossicida Pathogenicity and Epinephelus coioides’ Immune Response.doc by Zixu Liu (9204374)

“…The shRNA-406 mutant with the maximum silencing efficiency (97.5%) was chosen for further study. Compared with the wild-type (WT) P. plecoglossicida strain, silencing fliK in the fliK-RNA interference (RNAi) strain resulted in a significant decrease in growth, motility, chemotaxis, adhesion, and biofilm formation in P. plecoglossicida. …”

Image_4_Function of the fliK Gene in Pseudomonas plecoglossicida Pathogenicity and Epinephelus coioides’ Immune Response.pdf by Zixu Liu (9204374)

“…The shRNA-406 mutant with the maximum silencing efficiency (97.5%) was chosen for further study. Compared with the wild-type (WT) P. plecoglossicida strain, silencing fliK in the fliK-RNA interference (RNAi) strain resulted in a significant decrease in growth, motility, chemotaxis, adhesion, and biofilm formation in P. plecoglossicida. …”

Image_3_Function of the fliK Gene in Pseudomonas plecoglossicida Pathogenicity and Epinephelus coioides’ Immune Response.pdf by Zixu Liu (9204374)

“…The shRNA-406 mutant with the maximum silencing efficiency (97.5%) was chosen for further study. Compared with the wild-type (WT) P. plecoglossicida strain, silencing fliK in the fliK-RNA interference (RNAi) strain resulted in a significant decrease in growth, motility, chemotaxis, adhesion, and biofilm formation in P. plecoglossicida. …”

Table_2_Function of the fliK Gene in Pseudomonas plecoglossicida Pathogenicity and Epinephelus coioides’ Immune Response.doc by Zixu Liu (9204374)

“…The shRNA-406 mutant with the maximum silencing efficiency (97.5%) was chosen for further study. Compared with the wild-type (WT) P. plecoglossicida strain, silencing fliK in the fliK-RNA interference (RNAi) strain resulted in a significant decrease in growth, motility, chemotaxis, adhesion, and biofilm formation in P. plecoglossicida. …”

Image_2_Function of the fliK Gene in Pseudomonas plecoglossicida Pathogenicity and Epinephelus coioides’ Immune Response.pdf by Zixu Liu (9204374)

“…The shRNA-406 mutant with the maximum silencing efficiency (97.5%) was chosen for further study. Compared with the wild-type (WT) P. plecoglossicida strain, silencing fliK in the fliK-RNA interference (RNAi) strain resulted in a significant decrease in growth, motility, chemotaxis, adhesion, and biofilm formation in P. plecoglossicida. …”

Image_1_Function of the fliK Gene in Pseudomonas plecoglossicida Pathogenicity and Epinephelus coioides’ Immune Response.pdf by Zixu Liu (9204374)

“…The shRNA-406 mutant with the maximum silencing efficiency (97.5%) was chosen for further study. Compared with the wild-type (WT) P. plecoglossicida strain, silencing fliK in the fliK-RNA interference (RNAi) strain resulted in a significant decrease in growth, motility, chemotaxis, adhesion, and biofilm formation in P. plecoglossicida. …”

Retrotransposon expression in <i>Pld6</i> KO and <i>Dnmt3l</i> KO spermatocytes. by Kota Inoue (4282273)

“…Retrotransposons are grouped according to the degree of decreased methylation in <i>Dnmt3l</i> KO spermatogonia relative to WT spermatogonia. …”