Abberant α-Synuclein Confers Toxicity to Neurons in Part through Inhibition of Chaperone-Mediated Autophagy by Maria Xilouri (264240)

“…</p><h3>Methods and Findings</h3><p>Novel inducible SH-SY5Y and PC12 cell lines expressing human WT and A53T ASYN, as well as two mutant forms that lack the CMA-targeting motif were generated. …”

In the remaining proepicardium, <i>α4 integrin</i> expression and cell proliferation are reduced. by Yohei Saito (180130)

“…<p>(A–H, I and K) <i>In situ</i> hybridizations of sagital paraffin serial sections of E9.5 WT and <i>Mab21l2</i> mutant embryos for the transcripts indicated. …”

CV1720 cells show impaired ATR–dependent DNA damage responses. by Tomoo Ogi (122258)

“…<p>A) WT, DK0064 (ATR–SS), CV1720 (patient), CV1780 (patient's mother) and CV1783 (patient's father) cells were exposed to 5 Jm⁻² UV and the mitotic index (MI) assessed 2 h post exposure. …”

Data_Sheet_1_Mitral and Aortic Regurgitation in Patients Undergoing Kidney Transplantation: The Natural Course and Factors Associated With Progression.docx by Minjeong Kim (163958)

“…</p>Methods<p>Among patients who underwent KT in two tertiary centers, 430 (224 men, mean age 50 ± 13 years) were examined by echocardiography within 3 months before KT and between 6 and 36 months after KT. …”

Effect of apigenin intake on NF-κB activation in the dorsolateral prostate of TRAMP mice. by Sanjeev Shukla (106046)

“…<p><b>(A)</b> Protein expression of NF-κB/p65 and NF-κB/p50 as determined by Western blot analysis. A significant decrease in NF-κB/p65 and NF-κB/p50 is observed after apigenin intake. …”

Cell counts in different retinal layers for zebrafish exposed to MNU at baseline and follow-up. by Christoph Tappeiner (444164)

“…<p>A. MNU 50 mg/l group: No relevant decrease in retinal cells was observed (p>0.05). …”

Optimizing light-cured composite through variations in camphorquinone and butylhydroxytoluene concentrations by Hani NASSAR (12805999)

“…Sixteen groups of resin composite were prepared using combinations of four CQ (0.1%, 0.5%, 1.0%, and 1.5%) and four BHT (0.0%, 0.5%, 1.0%, and 1.5%) concentrations. …”

Identification of aa 100-200 as the transcriptional activation domain for WC-1. by Bin Wang (30851)

“…Both WT WC-1 and mutants were C-terminally tagged with V5. …”

Loss of Slfn3 induces a gender-dependent repair vulnerability after 50% bowel resection by Emilie DeKrey (9389738)

“…Mitotic marker, Phh3+, and proliferation marker expression of Lgr5, FoxL1, and PDGFRα were increased after resection in male WT but this effect was blunted in male Slfn3KO mice. …”

Effect of inhibitors of p38 MAPK or PKC on stimulated shedding by ADAM17T735A and ADAM17ΔC. by Katherine C. Hall (180988)

“…C) <i>Adam17−/−</i> mEFs transfected with TGFα together with WT ADAM17 were stimulated with 5 ng/ml IL-1β, 1 µM anisomycin or 25 ng/ml PMA in the presence or absence of 4 µM BIM I. …”

Myelin alteration in 8-week-old TRAF4-KO mice. by Sébastien Blaise (332585)

“…(C): Western blot analysis of the myelin-specific MBP marker and of the interglial-specific TJ marker claudin 11 (cl 11) showed a decreased expression level of both proteins in 4 independent TRAF4-KO cerebellum protein extracts (lanes 5–8) compared with 4 WT samples (lanes 1–4) ; tubulin served as a loading control. …”

Supplemental data of the manuscript “Accumulation of seminolipid in Sertoli cells is associated with increased levels of reactive oxygen species and male subfertility: Studies in a... by Kessiri Kongmanas (7467368)

“…</p> <p><b>Supplemental Figure S7</b> Levels superoxide anions (2HE) and other ROS + oxidants (Eth) of the cauda epididymis of 5-month-old <i>Arsa^-/-</i> mice and WT mice<b> (A)</b> and of the caput epididymis of 5-month-old and 8-month-old <i>Arsa^-/-</i> mice and age-matched WT mice <b>(B)</b>. …”

Rac2 promotes differentiation of M2 macrophages <i>in vitro</i>. by Shweta Joshi (555748)

“…(D) & (E), Heatmap representation of metabolites across BMDMs from WT (n = 5) and Rac2-/- (n = 5) mice. Shades of yellow represent elevation of a metabolite and shades of blue represent decrease of a metabolite relative to the median metabolite levels (see color scale). …”

SLS-4 is required for the <i>in vitro</i> ubiquitination of poly-SUMO-2 chains and degradation of SUMO conjugates <i>in vivo</i>. by Chris Boutell (205885)

“…<p>(A) <i>In vitro</i> ubiquitination reactions were carried out in the presence of E1, UbcH5a and ubiquitin (Ubmix), poly-SUMO-2 chains and purified wt ICP0, ICP0ΔRING, or ICP0 C-terminal truncation mutants ICP01-323, ICP01-396 or ICP01-396mSLS-4 as indicated (-/+). …”

Table_1.pdf by Hena Ahmad (4995290)

“…Both, western blot and confocal microscopy confirmed an increase of ataxin-2 in BE(2)-M17 cells transduced with lentivirus expressing mt-ELF2 (CEE-mt-ELF2), which was not observed in cells transduced with lentivirus expressing wt-ELF2 (CEE-wt-ELF2). Moreover, we observed a significant decrease in the number and size of lipid droplets in the CEE-mt-ELF2-transduced BE (2)-M17 cells, but not in the CEE-wt-ELF2-transduced BE (2)-M17. …”

Image_1.pdf by Hena Ahmad (4995290)

“…Both, western blot and confocal microscopy confirmed an increase of ataxin-2 in BE(2)-M17 cells transduced with lentivirus expressing mt-ELF2 (CEE-mt-ELF2), which was not observed in cells transduced with lentivirus expressing wt-ELF2 (CEE-wt-ELF2). Moreover, we observed a significant decrease in the number and size of lipid droplets in the CEE-mt-ELF2-transduced BE (2)-M17 cells, but not in the CEE-wt-ELF2-transduced BE (2)-M17. …”

Table_1_The Effects of Acute Aerobic Exercise on Blood Pressure, Arterial Function, and Heart Rate Variability in Men Living With HIV.DOCX by Juliana Pereira Barros (11136588)

“…NES; p < 0.05) reductions occurred in controls for SBP (∼10 mmHg, 2 h), DBP (∼6 mmHg, 20, 30, and 70 min), cSBP (∼9 mmHg, 30 min), cDBP (∼7 mmHg, 30 and 70 min), augmentation index (∼10%, 30 min), and pNN50 (∼20%; up to 2 h), while in MLHIV only cSBP (∼6 mmHg, 70 min) and cDBP (∼4 mmHg, 30 min) decreased. …”

Influence of T180A-SHIP2 on LEC functional responses. by Germaine D. Agollah (623510)

“…Data are presented as % of area covered by migrated cells on underside of transwell filters and cells stained with DRAQ5. (<b>D</b>) WT-SHIP2 exhibits increased adhesion (assessed by crystal violet staining) over Vector on BSA and collagen while T180A-SHIP2 shows decreased adhesion to collagen compared to WT. …”

<i>Mmp13</i>^−/− mice display increased bone volume during non-stabilized fracture healing. by Danielle J. Behonick (81485)

“…Scale bar = 1 mm (B) Histomorphometric measurements of total bone volume (BV) and total bone volume as a proportion of total callus volume (BV/TV) in WT and <i>Mmp13</i>^−/− mice at days 7 (WT <i>n</i> = 6, <i>Mmp13</i>^−/−<i>n</i> = 6), 10 (WT <i>n</i> = 8, <i>Mmp13</i>^−/−<i>n</i> = 6), 14 (WT <i>n</i> = 8, <i>Mmp13</i>^−/−<i>n</i> = 6), 21 (WT <i>n</i> = 6, <i>Mmp13</i>^−/−<i>n</i> = 6), 28 (WT <i>n</i> = 6, <i>Mmp13</i>^−/−<i>n</i> = 6) and 56 (WT <i>n</i> = 5, <i>Mmp13</i>^−/−<i>n</i> = 4) confirm this observation There is a statistically significant decrease in BV in <i>Mmp13</i>^−/− calluses compared with WT at day 7 (*p<0.05), but a statistically significant increase in BV and BV/TV in <i>Mmp13</i>^−/− calluses compared with WT at days 28 (**p<0.01 and p<0.05 respectively) and 56 (*p<0.05). …”

Rho-mediated control of <i>kinB</i> transcription is removed during sporulation. by Vladimir Bidnenko (4258573)

“…The highlighted points correspond to the WT profiles shown in panel (A): LBtran (orange), S1 (green), S5 (blue), S6 (pink); and RM (red) and WT (black) during exponential growth in LB. …”