Image_4_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Image_3_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Image_2_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Image_1_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Table_1_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.DOC by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Description of biomarkers, gene, primer sequences and NCBI accession numbers. by Conceição S. Martins (468492)

Effect of topical application of GSNO/HPMC formulation on histopathologic score of rat maxillae. by Conceição S. Martins (468492)

Effect of HPMC/GSNO on the nitrite/nitrate (NOX) levels in experimental periodontitis in rats. by Conceição S. Martins (468492)

Effect of topical application of HPMC/GSNO solutions on the plasma bone alkaline phosphatase (BAP) in experimental periodontitis in rats. by Conceição S. Martins (468492)

Quantification of iNOS, RANK, TRAP and nitrotyrosine immunostainning in periodontal tissue of rats submitted to experimental periodontitis. by Conceição S. Martins (468492)

Effect of topical application of HPMC/GSNO solutions on alveolar bone loss in experimental periodontitis. by Conceição S. Martins (468492)

Structure–Activity Relationships and Discovery of (<i>S</i>)‑5‑(<i>tert</i>-Butyl)-11-(difluoromethoxy)-9-methoxy-2-oxo-1,2,5,6-tetrahydropyrido[2′,1′:2,3]imidazo[4,5‑<i>h</i>]quin... by Dimitar Gotchev (1893928)

“…Optimization of a novel series of PAPD5/7 inhibitors that decrease HBsAg levels led to the tetracyclic 2-pyridone <b>AB-161</b>, which was similarly potent to <b>AB-452</b> <i>in vitro</i> and <i>in vivo</i> but showed dramatically higher rodent liver-to-plasma ratios. …”

BNT162b2- and infection induced reciprocal SARS-CoV-2 nAb titers on wildtype strain wt-BavPat1 and VOC strains Delta, Beta and Omicron at timepoint P1 to timepoint P3. by Olaf Nickel (9524791)

Effect of <i>slc43a3</i> deletion on physical activity of male and female WT and KO mice. by Aaron L. Sayler (19797446)

<i>elt-2</i> silencing causes decreased intestinal integrity on Bt679 but increased intestinal integrity on Bt247, while pathogen load remains the same as in controls. by Alejandra Zárate-Potes (9415842)

Do ecological characteristics drive the prevalence of <i>Panulirus argus</i> virus 1 (PaV1) in juvenile Caribbean spiny lobsters in a tropical reef lagoon? by Charlotte E. Davies (8506347)

“…<div><p>PaV1 is a pathogenic virus found only to infect Caribbean spiny lobsters <i>Panulirus argus</i>, a major fishing resource. …”

Fluctuations in airway bacterial communities associated with clinical states and disease stages in cystic fibrosis by Lisa A. Carmody (330117)

“…We sequenced the V3-V5 hypervariable region of the bacterial 16S rRNA gene in DNA derived from 631 sputum specimens collected from 111 CF patients over 10 years. …”

A, Uncropped image of gel in Fig 1B. by Tomokazu Yamaguchi (8284989)

Inactive <i>Hnf1α</i> targets in <i>Hnf1a^−/−</i> cells exhibit local enrichment of H3-Lys27me3, decreased H3-Lys4me2, and reduced DNAse I sensitivity. by Reini F. Luco (91817)

Chlorogenic acid (CGA) metabolites-containing serum from CGA-treated normal mice decreases lipid accumulation and stimulates cholesterol efflux from RAW264.7 cells. by Chongming Wu (169161)

“…<p>Normal C57BL/6J mice were orally gavaged with 400 mg/kg of CGA or equal volume of distilled water for 3 days and blood was collected at 45 min after the final treatment. 1% (v/v) of serum from CGA-treated normal mice (S_CGA) significantly decreased oxLDL-induced neutral lipid accumulation (A) and total cholesterol (B), and stimulates cholesterol efflux (C) in RAW264.7 cells as compared with that from distilled water-treated animals (S_NC). …”