microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration - Fig 6 by Surjyadipta Bhattacharjee (3181674)

“…After vacuum evaporation of HFIP, aliquoted peptide was re-suspended with DMSO to 5 mM and diluted to 5 μM into the cell culture media; <b>(B)</b> cells were treated with a scrambled miRNA-34a sequence (miRNA-34a-<b>sc</b>; 30 nM;, control, <b>a-d</b>, middle row); or <b>(C)</b> with an LNA-stabilized miRNA-34a (30 nM; miRNA-34a stressed; <b>a-d</b>; bottom row); treatments were for 24 hr before incubation with 5 uM of Aβ42 (made up as in [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref033" target="_blank">33</a>]) for another 24 hr before assay; MG cells were subsequently stained using a murine amyloid beta MABN10 (red fluorescence λ_max~650 nm; anti-Aβ antibody, clone W0-2; Millipore, Bellerica MA), a TREM-2 antibody (M-227): sc-48765 (green fluorescence; λ_max~510 nm; Santa Cruz, Santa Cruz CA) or DAPI nuclear stain (blue fluorescence; λ_max~470 nm) as in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a>;</b> arrows indicate Aβ42 uptake into MG cells; note decreased presence of TREM2 in miRNA-34a treated MG cells (bottom row, panel B) and decrease in ingested Aβ42 peptide within C8B4 cells (<b>C</b>; bottom row, panel <b>d</b>). …”

Characterization of serum cytokines during systemic infection with an <i>fgr41Δ/Δ</i> mutant. by Andrew S. Wagner (11331477)

High Pressure Study of Ru₃(CO)₁₂ by X-ray Diffraction, Raman, and Infrared Spectroscopy by Carla Slebodnick (90300)

“…The single-crystal X-ray structure of Ru₃(CO)₁₂ is reported at 8 pressures ranging from 1 atm (0.0 GPa) to 8.14(5) GPa. Although intramolecular bonding parameters remain relatively constant, intramolecular and intermolecular nonbonding contact distances decrease by an average of 4% and 15%, respectively. …”

Conditional <i>Cdk5rap2</i> KO mouse and novel <i>mCdk5rap2</i> splice variants. by Nadine Kraemer (790327)

“…(D) Identification of a novel <i>mCdk5rap2</i> splice variant (<i>mCdk5rap2-V1</i>) through gel electrophoresis and sequencing of PCR products from exon 2 to exon 7 of WT and hom KO cDNA. …”

Data_Sheet_1_Renal X-inactivation in female individuals with X-linked Alport syndrome primarily determined by age.PDF by Roman Günthner (8435811)

“…<p>X-linked Alport syndrome (AS) caused by hemizygous disease-causing variants in COL4A5 primarily affects males. Females with a heterozygous state show a diverse phenotypic spectrum ranging from microscopic hematuria to end-stage kidney disease (ESKD) and extrarenal manifestations. …”

Structural−Electronic Correlation in the First-Order Phase Transition of [FeH₂L^2-Me](ClO₄)₂ (H₂L^2-Me = Bis[((2-met... by Nicolas Bréfuel (2519947)

“…Variable-temperature magnetic susceptibility measurements show the occurrence of a steep spin crossover centered at 171.5 K with a hysteresis loop of ca. 5 K width ( =174 K and = 169 K, for increasing and decreasing temperatures, respectively). …”

Fast Pyrolysis of Tropical Biomass Species and Influence of Water Pretreatment on Product Distributions by Trevor James Morgan (787777)

“…The effect of pretreatment (i.e. reducing the amount of ash, and alkali and alkali earth metals) on pyrolysis products is: 1) to increase the dry bio-oil yield, 2) to decrease the amount of undetected material, 3) to produce a slight increase in CO yield or no change, 4) to slightly decrease CO₂ yield or no change, and 5) to produce a more stable bio-oil (less aging). …”

Effects of a Family-Based Childhood Obesity Treatment Program on Parental Weight Status by Cæcilie Trier (700327)

“…Both the mothers and fathers lost weight during their child’s treatment with a mean decrease in BMI in the mothers of 0.5 (95% CI: 0.2–0.8, <i>p</i> = 0.0006) and in the fathers of 0.4 (95% CI: 0.2–0.6, <i>p</i> = 0.0007). …”

Loss of <i>FGR41</i> or <i>CWP419</i> does not attenuate fungal growth <i>in vitro</i>. by Andrew S. Wagner (11331477)

Irradiating the lungs of young adult female FVB/N mice with either a single 12 Gy dose or fractionated 24 or 30 Gy doses of ¹³⁷Cs γ-rays leads to decreased levels of E-c... by Celine Almeida (289132)

“…<b>B, D</b>: After a fractionated 24 Gy dose, there is a slight increase in E-cadherin at 1 month postirradiation (PI) followed by a progressive decrease up to 5 months PI. …”

Orthostatic Hypotension and Elevated Resting Heart Rate Predict Low-Energy Fractures in the Population: The Malmö Preventive Project by Viktor Hamrefors (512698)

“…</p><p>The median follow-up time from baseline to first incident fracture among the subjects that experienced a low energy fracture was 15.0 years. A 10 mmHg orthostatic decrease in systolic blood pressure at baseline was associated with 5% increased risk of low-energy-fractures (95% confidence interval 1.01–1.10) during follow-up, whereas the resting heart rate predicted low-energy-fractures with an effect size of 8% increased risk per 10 beats-per-minute (1.05–1.12), independently of the orthostatic response. …”

Image_5_Group ICA of wide-field calcium imaging data reveals the retrosplenial cortex as a major contributor to cortical activity during anesthesia.jpg by Alessandro Scaglione (10810689)

Maps of average wet-day mean precipitation with units of mm/day (top) and relative linear trend in <i>μ</i> with units of %/decade (bottom) for the period 1950–2020, based on the E... by Rasmus E. Benestad (12530330)

Heavy metal EF values in five soil profiles. by Ziqi Li (7154915)

“…Except for profile HCZK02, the contents of 8 heavy metals generally decrease with increasing depth. As the depth of profile HCZK02 increases, As, Hg and Pb show a decrease-increase-decrease change; Cd and Cr show a decrease change and Ni shows a zigzag change; Cu and Zn show a decreasing-increasing-decreasing-increasing trend. …”

Characteristics of the microbial composition between resistance types. by Astri Dewayani (12878143)

Synonymous Rare Arginine Codons and tRNA Abundance Affect Protein Production and Quality of TEV Protease Variant by Jie Fang (306330)

“…<div><p>It has been identified that a TEV protease (TEVp) variant, TEVp^5M, displays improved solubility. …”

Performance of the model with the inhibitory V0_D pathways removed. by Yaroslav I. Molkov (542831)

“…Decreasing <i>α</i> causes two bifurcations: (i) transition from symmetric alternations of flexor activity, i.e. with a phase difference of Δ<i>φ</i> = 0.5, to alternations with a phase difference of Δ<i>φ</i> ≠0.5 at X₇ and then (ii) from alternation to synchronization of flexor activity at X₆. …”

The interaction of <i>F</i>. <i>tularensis</i> CipR and <i>kdsD</i>::<i>ltrB</i>_<i>L1</i> mutants with macrophage-like cells show a decrease in CFU recovery and disrupti... by Taylor Chance (611108)

Three-dimensional flow field of the Trp-cage folding. by Vladimir A. Andryushchenko (4662829)

Subject characteristics. by Astri Dewayani (12878143)

“…Following resistance status to five antibiotics, samples were classified into 24 sensitive, 24 single resistance, 16 double resistance, 5 triple resistance. …”