Effect of dietary squalene supplementation on plasma parameters in male <i>Apoa1</i>-deficient mice. by Clara Gabás-Rivera (613994)

Functional validation of a miRNA-34a-TREM2–3’UTR interaction. by Surjyadipta Bhattacharjee (3181674)

“…<p><b>(A)</b> ribonucleotide sequence of the 299 nt TREM2-mRNA-3’-UTR is shown in the 5’-3’ direction; the 22 nt miRNA-34a-TREM2 3’UTR complementarity-interaction region is indicated by a black underline and the 8 nt TREM2-mRNA-3’-UTR seed sequence <b>5’-ACACUGCU-3’</b> is overlaid in yellow; a single arrowhead indicates the 5’ end of a poly A+ tail in the TREM2 mRNA (22 ‘A’ nt shown; the length of this poly A+ tail is variable); TREM2 mRNA sequence derived from NM_018965; TREM2 transcript is the major X1 variant (see also <a href="http://switchdb.switchgeargenomics.com/productinfo/id_801321/" target="_blank">http://switchdb.switchgeargenomics.com/productinfo/id_801321/</a>) (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a></b>); <b>(B)</b> TREM2-mRNA-3’UTR expression vector luciferase reporter assay (pLightSwitch-3’UTR; Cat#S801178; Switchgear Genomics, Palo Alto CA); in this vector, the entire 299 nucleotide TREM2 3’UTR was ligated into the unique Nhe1-Xho1 site; <b>(C)</b> control C8B4 murine microglial cells, 1 week in culture; phase contrast bright field microscopy 20x; C8B4 cells transfected with the TREM2-mRNA-3’UTR expression vector luciferase reporter were treated exogenously with miRNA-34a, a scrambled control miRNA-34a (miRNA-sc) or control miRNA-183; see references and text for further details [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref018" target="_blank">18</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref019" target="_blank">19</a>]; <b>(D)</b> compared to control, C8B4 cells transfected with a scrambled (<b>sc</b>) control pLightSwitch-3’UTR vector, the TREM2-mRNA-3‘UTR vector exhibited decreased luciferase signal to a mean of 0.16-fold of controls in the presence of miRNA-34a; this same vector exhibited no change in the presence of the control miRNA-34a-sc or miRNA-183; for each experiment (using different batches of MG cells) a control luciferase signal was generated and included separate appropriate controls with each analysis; in addition a control vector β-actin-3’UTR showed no significant effects on the relative luciferase signal yield after treatment with either miRNA-183 or miRNA-34a (data not shown); dashed horizontal line set to 1.0 for ease of comparison; N = 5; *<i>p</i><0.001 (ANOVA). …”

Batroxobin accelerated tissue repair via neutrophil extracellular trap regulation and defibrinogenation in a murine ischemic hindlimb model by Haruchika Masuda (219184)

“…<div><p>Batroxobin, isolated from <i>Bothrops moojeni</i>, is a defibrinogenating agent used as a thrombin-like serine protease against fibrinogen for improving microcirculation. …”

<i>P</i>. <i>aeruginosa</i> biofilm formation is decreased by reducing potassium efflux from airway epithelial cells through inhibition of BK_Ca channels. by Glenn J. Rapsinski (6619841)

Cardiac adherent proliferating cells increase T regulatory cells, increase apoptotic CD4 and CD8 T cells, and decrease apoptotic T regulatory cells in Coxsackievirus B3-infected mi... by Kapka Miteva (192634)

Mean change in time spent with male ‘X’ in phase 1 vs phase 2 of expt. I. by Ronald David MacLaren (5220818)

“…<p>Mean change in total time (± SE) spent with male ‘X’ in phase 1 (in which no model female was used) vs phase 2 (wherein a model female was initially paired with the smaller male) in expt. …”

Onset of nilotinib resistance in K562 and K562-Dox cells coincides with ABCB1 overexpression; ABCB1 levels decrease upon continued high dose nilotinib exposure. by Laura N. Eadie (3044079)

(a) Geographic Location of China (Map sourced from the Ministry of Natural Resources of China, Carto-graphic License: GS (2019)1659); (b) Geographic Location of Xinxiang City; (c)... by Yichuan Zhang (2888345)

Absolute long-term trends 1901–2000 in mean annual surface air temperature and mean annual total precipitation for the global urban land (per grid cell) and the combined share of n... by Sebastian Scheuer (3142821)

“…<p>(A) Combined trends in mean annual surface air temperature (K) and mean annual total precipitation (mm) that are significant at the 10% level. …”

Printed Graphene Electrochemical Biosensors Fabricated by Inkjet Maskless Lithography for Rapid and Sensitive Detection of Organophosphates by John A. Hondred (3877561)

“…The IML-printed graphene electrode is nano/microstructured with a 1000 mW benchtop laser engraver and electrochemically deposited platinum nanoparticles (dia. ∼25 nm) to improve its electrical conductivity (sheet resistance decreased from ∼10 000 to 100 Ω/sq), surface area, and electroactive nature for subsequent enzyme functionalization and biosensing. …”

Cortical morphology of the femur at mid diaphysis (adjusted for bone mass), bone volume fraction (BV/TV) (adjusted for body mass), and trabecular microarchitecture of the distal fe... by Daksh Arora (16510065)

Histological quantification of ribosomal S6 phosphorylation in gray matter. by Dominique F. Leitner (12568901)

LAG3 deficiency enhances Th1 cells and decrease Treg upon adoptive transfer into wild-type mice followed by <i>E</i>. <i>multilocularis</i> infection. by Dewei Li (2806207)

Ribosomal S6 phosphorylation in whole brain homogenate. by Dominique F. Leitner (12568901)

AR-induced downstream signaling cascades were recapitulated in membrane-LRs of HUVECs cells, upon KSHV infection. by Xing Wang (154377)

“…<p>(a, b) PP1-induced decreasing expression of pEphA2 at Ser897 is rescued by DHT treatment upon KSHV infection. …”

Overtrained mice show reduced signaling capacity of DLS mGluR1/5 in response to a change in the prevailing A–O contingency. by Vincent Paget-Blanc (21807763)

LAG3 deficiency enhance Th1 cells and decrease Treg upon adoptive transfer into wild-type mice followed by <i>E</i>. <i>multilocularis</i> infection. by Dewei Li (2806207)

NPY neuron-specific Y2 deletion in the hypothalamus increases NPY and decreases POMC expression in the arcuate nucleus with no significant effects on body weight, spontaneous or fa... by Yan-Chuan Shi (153820)

Poly I:C increased HIF-1α and NF-κB which induced miR-210 leading to decreased STAT6 and IL-4 in human CTBs. by Shelley E. Kopriva (313874)

Citrulline plus SO scavenging by PEG-SOD+PEG-CAT provides complete protection. by Irene Gramaglia (6439613)