microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration - Fig 6 by Surjyadipta Bhattacharjee (3181674)

“…After vacuum evaporation of HFIP, aliquoted peptide was re-suspended with DMSO to 5 mM and diluted to 5 μM into the cell culture media; <b>(B)</b> cells were treated with a scrambled miRNA-34a sequence (miRNA-34a-<b>sc</b>; 30 nM;, control, <b>a-d</b>, middle row); or <b>(C)</b> with an LNA-stabilized miRNA-34a (30 nM; miRNA-34a stressed; <b>a-d</b>; bottom row); treatments were for 24 hr before incubation with 5 uM of Aβ42 (made up as in [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref033" target="_blank">33</a>]) for another 24 hr before assay; MG cells were subsequently stained using a murine amyloid beta MABN10 (red fluorescence λ_max~650 nm; anti-Aβ antibody, clone W0-2; Millipore, Bellerica MA), a TREM-2 antibody (M-227): sc-48765 (green fluorescence; λ_max~510 nm; Santa Cruz, Santa Cruz CA) or DAPI nuclear stain (blue fluorescence; λ_max~470 nm) as in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a>;</b> arrows indicate Aβ42 uptake into MG cells; note decreased presence of TREM2 in miRNA-34a treated MG cells (bottom row, panel B) and decrease in ingested Aβ42 peptide within C8B4 cells (<b>C</b>; bottom row, panel <b>d</b>). …”

Total blood cell counts are decreased in the irradiated (2 Gy) groups +/− HU treatment WBCs (A) and lymphocytes (B) at 4 days post-proton radiation exposure are significantly decreased, compared to the No treatment group (*). by Jenine K. Sanzari (306909)

“…Average counts +/− SD are expressed as a fraction of the control, n = 3–5. The neutrophil count at 4 days post-proton radiation exposure + HU treatment are significantly decreased, compared to the neutrophil counts observed for the No treatment group (*) and the HU group (#). …”

Heavy metals concentration in ammonia removal rate; as % of the source (ARRS) and ammonia removal rate; as % of the control (ARRC) of different treatments (Means± SE). by Ghada R. Sallam (18319994)

A pear orchard in Quillón. by Jeannette Silva-López (16541854)

Genome-Wide Association Study with Targeted and Non-targeted NMR Metabolomics Identifies 15 Novel Loci of Urinary Human Metabolic Individuality by Johannes Raffler (793347)

“…In contrast, for the <i>SLC5A11</i> locus, we found increased levels of <i>myo</i>-inositol in urine whereas mGWAS in blood reported decreased levels for the same genetic variant. …”

Twenty-two identified and replicated loci and their overlap with associations to metabolic traits and clinical phenotypes. by Johannes Raffler (793347)

“…We added their proxy variants in LD (r² ≥ 0.8; based on 1000 genomes project data [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005487#pgen.1005487.ref050" target="_blank">50</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005487#pgen.1005487.ref051" target="_blank">51</a>]). …”

Open data. by Larissa Brazolotto Ferreira (17671168)

“…</p><p>Results</p><p>There was a significant decrease in BMD in the lumbar spine (1.107 ± 0.109 and 1.075 ± 0.112 g/cm²; <i><i>p</i></i> < 0.001), total body (1.135 ± 0.086 and 1.119 ± 0.085 g/cm²; <i><i>p</i></i> < 0.001), and total proximal femur over the six months of EBF compared to the values obtained from the same EBF group at 15 days postpartum (mean percentage decreases in BMD of −3.4 ± 3.7% (<i><i>p</i></i> < 0.001) in lumbar spine, −2.5 ± 3.4% (<i><i>p</i></i> < 0.001) in total proximal femur, and −1.7 ± 1.9% (<i><i>p</i></i> = 0.001) in total body). …”

Prediction of the effects of HNF1A missense mutations on protein stability. by Sneha P. (3926957)

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Number of intermolecular hydrogen bonds formed between the protein and DNA in the native and mutant complexes. by Sneha P. (3926957)

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Three-dimensional structure of the HNF1A protein (PDB ID-1IC8) shows the POU_H (marked in pink) and POU_S (marked in blue) domains interacting with DNA. by Sneha P. (3926957)

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Determining the role of missense mutations in the POU domain of HNF1A that reduce the DNA-binding affinity: A computational approach - Fig 9 by Sneha P. (3926957)

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Docking analysis scores for native and mutant (R131W, R131Q, and R203C) complexes from the HADDOCK server. by Sneha P. (3926957)

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Free energy landscape between PC1 and PC2 of native and mutant complexes. by Sneha P. (3926957)

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Block-based RMSD analysis of the native protein-DNA complex. by Sneha P. (3926957)

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The radius of gyration analysis of native and mutant complexes. by Sneha P. (3926957)

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Backbone RMSD graph of the native and mutant complexes. by Sneha P. (3926957)

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The ConSurf analysis. by Sneha P. (3926957)

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List of potential pathogenic HNF1A missense mutations predicted by all <i>in silico</i> prediction tools. by Sneha P. (3926957)

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Determining the role of missense mutations in the POU domain of HNF1A that reduce the DNA-binding affinity: A computational approach by Sneha P. (3926957)

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Variant Histone H2afv reprograms DNA methylation during early zebrafish development by Bhavani Madakashira (4570399)

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