Loss of ATRX and SLF2 enables telomere exchanges. by William A. Scott (11707932)

“…<p>A: DNA sequencing and western blotting confirming the disruption of ATRX expression in CRISPR-Cas9-edited HEK293 Flp-In T-REx cells inducibly expressing RAP1-BirA*. …”

Label-Free Interactome Analysis Revealed an Essential Role of CUL3-KEAP1 Complex in Mediating the Ubiquitination and Degradation of PHD2 by Ang Luo (8097296)

“…Prolyl hydroxylase domain-containing protein 2 (PHD2/EGLN1) is a key regulatory enzyme that plays a fundamental role in the cellular hypoxic response pathway, mediating proline hydroxylation-dependent protein degradation of selected target proteins. …”

Phosphorylation of p27^Kip1 at S140 regulates its protein stability in the G1-phase of the cell cycle. by Erica K. Cassimere (3111996)

“…<p>(A) Ectopically expressed p27^Kip1 S140A mutant has a decreased half-life compared with the WT or phosphomimetic (S140D) versions. …”

Comparative Transcriptomic Analysis of Primary Duck Hepatocytes Provides Insight into Differential Susceptibility to DHBV Infection by Liang Yan (494300)

“…After re-annotation against human genome data, the 384 DEGs were pooled together with proteins belonging to hepatitis B pathway to construct a protein-protein interaction network. The combination of decreased expression of liver-specific genes (CYP3A4, CYP1E1, CFI, RELN and GSTA1 et al) with increased expression of hepatocyte-dedifferentiation-associated genes (PLA2G4A and PLCG1) suggested that <i>in vitro</i> culture conditions results in the fading of hepatocyte phenotype in PDHs. …”

mGluR1 activity regulates cell proliferation. by Malathi Banda (505556)

“…<p><b>A. Proliferation.</b> mGluR1 overexpression significantly increased proliferation of MCF10AT1 cells compared to the control as measured by MTT assay. …”

Immunomodulation by gastrointestinal carbon black nanoparticle exposure in ovalbumin T cell receptor transgenic mice by Jason H. Fine (3109035)

“…Exposure to OVA resulted in significant increases in serum anti-OVA IgG1, anti-OVA IgM, and anti-OVA IgA antibodies relative to vehicle control. Immunophenotyping revealed a reduction in the number of OVA-specific CD4⁺T helper cells upon OVA ± CBNP^HI treatment in the spleen. …”

Source data. by Shihao Zhou (5774795)

“…Under wide-field imaging in primary somatosensory and visual cortices in mice with high labeling density (80–90%), SomaFRCaMPi exhibits up to 40% higher SNR and decreased artifactual correlation across neurons. Altogether, SomaFRCaMPi improves the accuracy and scale of neuronal activity imaging at single-neuron resolution in densely labeled brain tissues due to a 2–3-fold enhanced automated neuronal segmentation, 50% higher fraction of responsive cells, up to 2-fold higher SNR compared to jRGECO1a. …”

In vitro biophysical properties of red GECIs. by Shihao Zhou (5774795)

“…Under wide-field imaging in primary somatosensory and visual cortices in mice with high labeling density (80–90%), SomaFRCaMPi exhibits up to 40% higher SNR and decreased artifactual correlation across neurons. Altogether, SomaFRCaMPi improves the accuracy and scale of neuronal activity imaging at single-neuron resolution in densely labeled brain tissues due to a 2–3-fold enhanced automated neuronal segmentation, 50% higher fraction of responsive cells, up to 2-fold higher SNR compared to jRGECO1a. …”

Source data. by Shihao Zhou (5774795)

“…Under wide-field imaging in primary somatosensory and visual cortices in mice with high labeling density (80–90%), SomaFRCaMPi exhibits up to 40% higher SNR and decreased artifactual correlation across neurons. Altogether, SomaFRCaMPi improves the accuracy and scale of neuronal activity imaging at single-neuron resolution in densely labeled brain tissues due to a 2–3-fold enhanced automated neuronal segmentation, 50% higher fraction of responsive cells, up to 2-fold higher SNR compared to jRGECO1a. …”

Waterborne exposure to BPS causes thyroid endocrine disruption in zebrafish larvae by Dan-hua Zhang (3997844)

“…<div><p>Bisphenol S (BPS) is widely used as a raw material in industry, resulting in its ubiquitous distribution in natural environment, including the aqueous environment. …”

Pharmacology and Therapeutic Potential of Benzothiazole Analogues for Cocaine Use Disorder by Comfort A. Boateng (3617120)

“…This study focused on the synthesis and evaluation of a novel series of benzothiazole analogues designed to target D₄R. …”

NRG1-II stimulates neurogenesis through ErbB4 as a cell-extrinsic signal. by Tomomi Sato (301255)

“…The approximate site of the section is shown in a lateral view in the inset. <b>E</b>. A decrease in Tyr¹¹⁶²-phosphorylated ErbB4 (pErbB4) in the OT of MO^{<i>nrg1IIE1</i>}-injected embryos (<i>nrg1IIE1</i>) compared to the control MO^{<i>nrg1IIE15m</i>}-injected embryos (<i>nrg1IIE15m</i>) at 40 hpf. …”

MMV291 disrupts actin-dependent apicoplast segregation and induces a partial delayed death phenotype. by Madeline G. Dans (14193815)

“…<b>ii</b> These images were quantified by 3 independent blind scorers, which showed a significant decrease in the normal segregation of apicoplasts between the 10 μM MMV291 and DMSO treatments, while the 5 μM MMV291 was not significant (ns). …”

Knockdown of Appa, Appb, or Prp1 results in impaired development and death of head region. A–L. by Darcy M. Kaiser (114856)

“…S3</a>). The <i>appa</i> and <i>appb</i> splice blocking (SB) MOs used above (A–H) significantly decreases detection of protein species by the antibody 22C11 (top row). …”

A randomized controlled trial and prospective cohort investigating antivenom for red-bellied black snake envenomation by Geoffrey K. Isbister (8281572)

“…</p> <p>Administration of antivenom in red-bellied black snake envenomation within 6 hours post-bite appeared to decrease the proportion of patients with myotoxicity, but a third of patients had adverse reactions.…”

MHC Ib molecule Qa-1 presents <i>Mycobacterium tuberculosis</i> peptide antigens to CD8⁺ T cells and contributes to protection against infection by Yao Bian (2820964)

“…T cells from Qa-1^-/- mice also had increased expression of inhibitory and apoptosis-associated cell surface markers such as CD94/NKG2A, KLRG1, PD-1, Fas-L, and CTLA-4. As such, they were more prone to cell death and had decreased capacity in promoting the killing of Mtb in infected macrophages. …”

Stabilization of p27^Kip1 through S140 phosphorylation is required for tumorsphere resistance to IR. by Erica K. Cassimere (3111996)

“…Differences between groups were evaluated using two-tailed Student <i>t</i> tests among replicate experiments; **P = 0.005. (E) p27S140A has a decreased half-life compared to p27WT in tumorspheres subjected to IR. …”

Cleavage of osmosensitive transcriptional factor NFAT5 by Coxsackieviral protease 2A promotes viral replication by Ye Qiu (363315)

“…In this study, we aimed to understand the mechanism by which NFAT5 interferes with infection of Coxsackievirus B3 (CVB3), a major cause of viral myocarditis. Our initial results demonstrated that although the mRNA level of NFAT5 remained constant during CVB3 infection, NFAT5 protein level decreased because the protein was cleaved. …”

Functional regulatory mechanism of smooth muscle cell-restricted <i>LMOD1</i> coronary artery disease locus by Vivek Nanda (746334)

“…<i>LMOD1</i> knockdown results in increased proliferation and migration and decreased cell contraction in HCASMC, and immunostaining in atherosclerotic lesions in the SMC lineage tracing reporter mouse support a key role for LMOD1 in maintaining the differentiated SMC phenotype. …”

mGluR1 facilitates anchorage-independent growth. by Malathi Banda (505556)

“…<p><b>A and B. MCF10AT1 cells.</b> Cells were transduced with either with pLenti-<i>GRM1</i>, pLenti-<i>LacZ</i>, sh<i>GRM1</i>, or NS control vector and stably selected before plating in soft agar. …”