Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

File S1 - Post-Transcriptional Regulator Hfq Binds Catalase HPII: Crystal Structure of the Complex by Koji Yonekura (480516)

“…Decomposition of hydrogen peroxide (H₂O₂) over time shows the decrease in absorbance at λ = 240 nm. Time = 0 indicates a point just after H₂O₂ was added. …”

Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Integrity of AWC cilia structure is critical for proper localization of EGL-4. by Damien M. O'Halloran (185919)

“…Calcium levels in AWC have been shown to decrease after ligand binding <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000761#pgen.1000761-Chalasani1" target="_blank">[13]</a>. …”

Experimental tasks and model framework. by Jana Masselink (16764941)

“…The visuomotor system evaluates the accuracy of the motor command with respect to the postdicted target position (<i>E</i>_<i>post</i>) in order to adapt its gains <i>ω</i>_<i>v</i>, <i>ω</i>_<i>m</i> and <i>ω</i>_<i>cd</i>. A decrease of <i>ω</i>_<i>m</i> (inward learning) is controlled by downregulating saccade peak velocity while an increase of <i>ω</i>_<i>m</i> (outward learning) is controlled by upregulation of saccade duration. …”

Reversal of ROS induced autophagic vacuole formation in F-HABP07 upon polymeric HA supplementation. by Paramita Saha (473954)

“…<p>[<b>A to D</b>] <b><i>Polymeric </i><i>HA </i><i>supplementation </i><i>restores </i><i>cellular </i><i>homeostasis </i><i>in </i><i>F-HABP07 </i><i>cells</i></b>-- As a result of significant reduction in ROS generation as evident from the decreased fluorescence intensity upon H₂DCFDA treatment (<b>A</b> and <b>B</b>); about 25% revival of growth was observed in F-HABP07 cells (<b>C</b>). …”

Proof of Concept, Randomized, Placebo-Controlled Study of the Effect of Simvastatin on the Course of Age-Related Macular Degeneration by Robyn H. Guymer (168403)

“…<i>Intent to treat</i> multivariable logistic regression analysis, adjusted for age, sex, smoking and baseline AMD severity, showed a significant 2-fold decrease in the risk of progression in the simvastatin group: OR 0.43 (0.18–0.99), p = 0.047. …”

Data_Sheet_1_Coordinated Reset Vibrotactile Stimulation Induces Sustained Cumulative Benefits in Parkinson’s Disease.pdf by Kristina J. Pfeifer (10095703)

“…EEG recordings observed from study 1 indicated a significant decrease in off-medication cortical sensorimotor high beta power (21—30 Hz) at rest after 3 months of daily noisy vCR therapy. …”

Image_1_Defective Autophagy in T Cells Impairs the Development of Diet-Induced Hepatic Steatosis and Atherosclerosis.TIF by Jacob Amersfoort (6075341)

“…Taken together, T cell-specific deficiency of Atg7 resulted in a decrease in hepatic steatosis and limited inflammatory potency in the (naïve) T cell compartment in peripheral lymphoid tissues, which was associated with a strong reduction in experimental atherosclerosis.…”

Knockdown of miRs fails to suppress translational activation of TOP mRNAs. by Ilona Patursky-Polischuk (648177)

“…<p>(A) MDA-MB-231 cells were infected with lentivirus expressing either anti-miR 10b sponge or anti-miR-BART 1–5p sponge (control). …”

Absence of LAMP5 disrupts olfaction and leads to anxiety-like behavior. by Marie-Catherine Tiveron (272288)

“…Habituation occurs similarly for both WT and KO mice as shown by a significant decrease in investigation time between the first (Oh1) and last (Oh4) trials (*, p<0.05; **, p<0.01; ***, p<0.001). …”

Progressive differences between IPCs and OC surface proportions promoted 2° and 3° formation. by David E. Larson (246516)

“…‘No OC Expansion’, No Death' curve indicates constant OC surface area (no expansion) and no cell death. (<b>C</b>) A graph indicating the decrease in cell number for the different simulations. …”

<i>NDUFS7</i> depletion in motor neurons affects synapse stability. by Bhagaban Mallik (12459375)

“…The motor neuron-depleted <i>NDUFS7[RNAi]</i> larvae showed a decrease in the number of Futsch-positive loops as compared to the Gal4 control. …”

Defect Tolerance to Intolerance in the Vacancy-Ordered Double Perovskite Semiconductors Cs₂SnI₆ and Cs₂TeI₆ by Annalise E. Maughan (1694959)

“…However, even though tellurium substitution increases electronic dispersion via closer I–I contact distances, the substitution experimentally yields insulating behavior from a significant decrease in carrier concentration and mobility. …”

SIRT5 proviral activity is independent of the MAVS signaling pathway. by Marius Walter (13775591)

“…<p><b>A</b>. Western blot showing 90% reduction of MAVS in A549-A/T cells transduced with a CRISPR lentivirus against MAVS. …”

Pi across filarial nematode species and model organisms. by John Mattick (544773)

“…<i>melanogaster</i> also has a decrease in Pi; it is a small chromosome sometimes referred to as the dot chromosome that is largely heterochromatic and may formerly have been a sex chromosome [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0009838#pntd.0009838.ref074" target="_blank">74</a>].…”

Defect Tolerance to Intolerance in the Vacancy-Ordered Double Perovskite Semiconductors Cs₂SnI₆ and Cs₂TeI₆ by Annalise E. Maughan (1694959)

“…However, even though tellurium substitution increases electronic dispersion via closer I–I contact distances, the substitution experimentally yields insulating behavior from a significant decrease in carrier concentration and mobility. …”

Data_Sheet_5_Personalized surgical planning for coronary bypass graft configurations using patient-specific computational modeling to avoid flow competition in arterial grafts.XLSX by Krish Chaudhuri (13866956)

“…The use of the computational models led to a significant decrease in the selection of unsatisfactory grafting configurations when surgeons employed “anaortic” (21.25% (17/80) vs. 1.25% (1/80), P < 0.001) but not “aortic” techniques (5% (4/80) vs. 0% (0/80), P = 0.64). …”

Prediction performance of each model. by Ahmed S. Alkhalifah (20721713)

“…Further studies to define a safe upper limit for IBW-adjusted MP may help clinicians optimize mechanical ventilation settings and decrease the risk of VILI and mortality.…”