Hsp70 allosteric cycle. by Ignacio J. General (564941)

“…The co-chaperones, DnaJ and GrpE, assist in the hydrolysis and nucleotide exchange steps, respectively. Diagrams <b>B</b> and <b>D</b> were generated using the PDB files 1DKX <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003624#pcbi.1003624-Zhu1" target="_blank">[7]</a> and 4B9Q <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003624#pcbi.1003624-Kityk1" target="_blank">[25]</a>, respectively. …”

DataSheet_2_Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology.pdf by Sandrine Schmutz (2922075)

“…We conclude that spectral flow cytometry is a highly valuable tool for high-end immunophenotyping, and that fine-tuning of major experimental steps is key for taking advantage of its full capacity.…”

DataSheet_5_Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology.pdf by Sandrine Schmutz (2922075)

“…We conclude that spectral flow cytometry is a highly valuable tool for high-end immunophenotyping, and that fine-tuning of major experimental steps is key for taking advantage of its full capacity.…”

DataSheet_3_Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology.pdf by Sandrine Schmutz (2922075)

“…We conclude that spectral flow cytometry is a highly valuable tool for high-end immunophenotyping, and that fine-tuning of major experimental steps is key for taking advantage of its full capacity.…”

DataSheet_4_Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology.pdf by Sandrine Schmutz (2922075)

“…We conclude that spectral flow cytometry is a highly valuable tool for high-end immunophenotyping, and that fine-tuning of major experimental steps is key for taking advantage of its full capacity.…”

DataSheet_1_Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology.pdf by Sandrine Schmutz (2922075)

“…We conclude that spectral flow cytometry is a highly valuable tool for high-end immunophenotyping, and that fine-tuning of major experimental steps is key for taking advantage of its full capacity.…”

Dibenzo[<i>hi</i>,<i>st</i>]ovalene as Highly Luminescent Nanographene: Efficient Synthesis via Photochemical Cyclodehydroiodination, Optoelectronic Properties, and Single-Molecule... by Qiang Chen (114038)

“…Remarkably, the intersystem crossing rate into the triplet state decreased by more than an order of magnitude at low temperature, demonstrating that temperature can be a crucial parameter to boost single photon emission of an aromatic hydrocarbon.…”

Dibenzo[<i>hi</i>,<i>st</i>]ovalene as Highly Luminescent Nanographene: Efficient Synthesis via Photochemical Cyclodehydroiodination, Optoelectronic Properties, and Single-Molecule... by Qiang Chen (114038)

“…Remarkably, the intersystem crossing rate into the triplet state decreased by more than an order of magnitude at low temperature, demonstrating that temperature can be a crucial parameter to boost single photon emission of an aromatic hydrocarbon.…”

Phospholipase D1 Couples CD4⁺ T Cell Activation to c-Myc-Dependent Deoxyribonucleotide Pool Expansion and HIV-1 Replication by Harry E. Taylor (596259)

“…These findings uncover a unique mechanism of action for PLD1 inhibitors and support their further development as part of a therapeutic combination for HIV-1 and other viral infections dependent on host nucleotide biosynthesis.…”

Rab3D Is Critical for Secretory Granule Maturation in PC12 Cells by Tanja Kögel (391540)

“…This maturation process is characterized by acidification-dependent processing of cargo proteins, condensation of the SG matrix and removal of membrane and proteins not destined to mature secretory granules (MSGs). Here we addressed a potential role of Rab3 isoforms in these maturation steps by expressing their nucleotide-binding deficient mutants in PC12 cells. …”

Municipal Policy of Basic Sanitation and the occurrence of diseases in Brazilian municipalities by Natássia Molina Bayer (12849878)

“…The results of the second stage showed that there is a decreased chance of disease occurrence in municipalities that belong to HH clusters (municipalities with PMSB, surrounded by municipalities with PMSB) and LH clusters (municipalities without PMSB, surrounded by municipalities with PMSB). …”

Protein amounts and western blot analysis of lysates extracted from lymph node samples fixed for 6 h, 24 h, 48 h and 144 h, respectively. by Claudia Wolff (149167)

“…<p>For a short fixation time no real difference could be detected between the two buffers tested. …”

Extracellular Heat Shock Protein (Hsp)70 and Hsp90α Assist in Matrix Metalloproteinase-2 Activation and Breast Cancer Cell Migration and Invasion by Jessica D. Sims (224825)

“…<div><p>Breast cancer is second only to lung cancer in cancer-related deaths in women, and the majority of these deaths are caused by metastases. Obtaining a better understanding of migration and invasion, two early steps in metastasis, is critical for the development of treatments that inhibit breast cancer metastasis. …”

Dissociations between meta-d’ and d’. by Medha Shekhar (19502698)

“…<p>We tested the three networks (4-layer CNN, VGG-19 and ResNet-50) on the task paradigm from Maniscalco et al. (2016) [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1012578#pcbi.1012578.ref018" target="_blank">18</a>] which demonstrated a dissociation between d’ and meta-d’ when the contrast of one stimulus category (S₁) remains fixed while the contrast of the other stimulus is increased in discrete steps (S₂) For this design, meta-d’ increases with d’ as expected for trials in which the observer responds "S₂", but meta-d’ decreases with d’ for trials where the observer responds "S₁". …”

The cyclophilin inhibitor CRV431 inhibits liver HBV DNA and HBsAg in transgenic mice by Philippe Gallay (239012)

“…We found that oral treatment with CRV431 (50 mg/kg/day) for a period of 16 days significantly reduced liver HBV DNA levels and moderately decreased serum HBsAg levels. …”

Effects of active SA with RyR calcium exchange mechanisms only. by James Rosado (12456467)

“…It is worth observing that Ca²⁺ coupling is significantly decreased. In Fig 5C, 5D, and 5E, we demonstrate for spine 8 that there is a critical RyR density such that Ca²⁺ coupling occurs in the dendritic region. …”

Truncated and Helix-Constrained Peptides with High Affinity and Specificity for the cFos Coiled-Coil of AP-1 by Tara Rao (395307)

“…They competitively antagonized the cJun–cFos coiled-coil interaction. Truncating a Jun-based peptide from 37 to 22 residues decreased the binding enthalpy for cJun by ∼9 kcal/mol, but this was compensated by increased conformational entropy (TΔS ≤7.5 kcal/mol). …”

Guanine metabolism. by Patricia Kuzaj (635687)

“…<p>(a) Guanine levels were detected by LC-MS/MS in positive ionization mode (patient/control ratio:2.4, p<0.0001). …”

Activation of CCAP and CAMB neurons initiates ecdysis behavior. by Do-Hyoung Kim (799666)

“…<p>(A) Held at a constant 24°C, the natural ecdysis FAP observed in puparium-free control (<i>w</i>^<i>1118</i>) and two test groups (<i>CCAP-Gal4>UAS-TRPM8</i>, <i>Pburs-Gal4>UAS-TRPM8</i>) shows normal scheduling of behavioral steps. …”

Axisymmetric and Nonaxisymmetric Oscillations of Sessile Compound Droplets in an Open Digital Microfluidic Platform by Shubhi Bansal (4491013)

“…Manipulating droplets of biological fluids in an electrowetting on dielectric (EWOD)-based digital microfluidic platform is a significant challenge because of biofouling and surface contamination. …”