Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution by Massimiliano Gaetani (241969)

“…Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. …”

Ubr3, a Novel Modulator of Hh Signaling Affects the Degradation of Costal-2 and Kif7 through Poly-ubiquitination by Tongchao Li (115391)

“…In zebrafish, loss of Ubr3 causes a decrease in Shh signaling in the developing eyes, somites, and sensory neurons. …”

Did B inhibits early HIV-1 replication by targeting the RT-eEF1A interaction. by Dongsheng Li (222712)

“…HIV-1 containing a W252A RT mutant has decreased ability to interact with eEF1A. …”

CYP gene expression of indoxyl sulfate (IS) in primary human hepatocytes. by Masao Togao (21696599)

Subjects:

CYP3A activity in DPX-2 cells treated with each test compound. by Masao Togao (21696599)

Subjects:

CYP3A activity in HepaRG^® cells treated with each test compound. by Masao Togao (21696599)

Subjects:

CYP3A activity in primary human hepatocytes treated with indoxyl sulfate (IS). by Masao Togao (21696599)

Subjects:

Directed Evolution of RecA Variants with Enhanced Capacity for Conjugational Recombination by Taejin Kim (472963)

“…Relative to wild type RecA, the selected RecA variants exhibited faster rates of ATP hydrolysis, more rapid displacement of SSB, decreased inhibition by the RecX regulator protein, and in general displayed a greater persistence on DNA. …”

Fig 2A–2C and 2G–2L Data. by Kevin D. Gaitonde (15411707)

“…We observed a marked decrease in nocturnal locomotion of <i>Opn5</i>^<i>cre</i><i>; Gnas</i>^<i>fl/fl</i> mice at both 28°C and 22°C, but no overall differences in energy expenditure, respiratory exchange, or food and water consumption. …”

<i>Trypanosoma cruzi</i> transmission dynamics in a synanthropic and domesticated host community by Alheli Flores-Ferrer (2151817)

“…To understand its transmission dynamics in synanthropic and domesticated species living within villages is essential to quantify disease risk and assess the potential of zooprophylaxis. We developed a <i>SI</i> model of <i>T</i>. <i>cruzi</i> transmission in a multi-host community where vector reproduction and parasite transmission depend on a triatomine blood-feeding rate accounting for vector host preferences and interference while feeding. …”

Anti-CXCR3 group decreased number of specific CD8⁺ T cells as well as polyfunctionality after infection with <i>T</i>. <i>cruzi</i>. by Camila Pontes Ferreira (6194006)

Overexpression of Atp13a2 protects cells from manganese cytotoxicity. by Janet Ugolino (3359054)

“…Graph is representative of three independent experiments and data is shown as mean ±SDM. Cells expressing Atp13a2 show a significant decrease in cell death compared to normal HeLa cells after exposure to MnCl₂. …”