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Measured anisotropy in different illumination modes, for analysis receptor assemblies in the cell membrane.
Published 2014“…(iv) Upon agonist treatment, the labeled P2Y1 receptors readily form dimers in the plasma membrane with almost 100% efficiency. (v) Application of a highly potent P2Y1R selective antagonist, completely reverses the agonist-induced decrease in anisotropy in the TIRF mode, confirming the P2Y1R involvement.…”