8oxoG and AP lesions disrupt TRF2 recognition of telomeric DNA by Patricia L. Opresko (7493)

“…All rights reserved</p> Boxes indicate the GGG runs of the tandem telomeric repeats, and (X) marks the position of 8oxoG () or abasic () lesion(s) within the G runs (see ). Substrates (2.5 nM) were incubated alone (−) or together with decreasing concentrations of TRF2 (200, 100, 50 or 25 nM) for 20 min at 4°C in TRF2 binding buffer. …”

Localization of LMNB1 during TGC formation. by Hiromu Morimoto (11133531)

“…The amount of LMNB1 protein peaked at day 3 and then gradually decreased. The data are represented as means ± SE (n = 5). …”

Alteration of metabolic profiles in <i>Lemna paucicostata</i> culture and enhanced production of GABA and ferulic acid by ethephon treatment by EunBi Kim (6179564)

“…<div><p><i>Lemna</i> species have been used in the food, feed, and pharmaceutical industries, as they are inexpensive sources of proteins, starches, and fatty acids. In this study, we treated <i>L</i>. <i>paucicostata</i> with different concentrations (0.05, 0.1, 0.2, 0.5, or 1 mM) of ethephon. …”

DataSheet1_Ocean acidification enhances the tolerance of dinoflagellate Prorocentrum donghaiense to nanoplastic-induced oxidative stress by modulating photosynthetic performance.do... by Yue Zhu (306128)

“…</p>Results and discussion<p>The findings revealed that NPs inhibited the growth of P. donghaiense by inducing oxidative stress, as indicated by elevated malondialdehyde (MDA) content and decreased carotenoid/chlorophyll-a ratio, even though photochemical efficiency (φ_P0, ψ₀, and φ_E0), rETR_max and α were enhanced in response to NPs stress. …”

Association Mapping Provides Insights into the Origin and the Fine Structure of the Sorghum Aluminum Tolerance Locus, <i>Alt_SB</i> by Fernanda F. Caniato (217481)

Disruption of <em>Chtf18</em> Causes Defective Meiotic Recombination in Male Mice by Karen M. Berkowitz (123502)

“…<em>Chtf18</em>-null males exhibit 5-fold decreased sperm concentrations compared to wild-type controls, resulting in subfertility. …”

Table_1_Insights into land size and productivity in Ethiopia: What do data and heterogenous analysis reveal?.docx by Ashok K. Mishra (452738)

“…Finally, the quantile regression reveals interesting findings. These are: (1) a strong positive effect of farm (and plot) size on productivity; (2) the magnitude of the effect decreases monotonically with quantile; (3) farm size displays a robust negative impact on gross revenue and the magnitude of the effect increases (in absolute terms) monotonically with quantiles; (4) the effect of farm (and plot) size on productivity decreases in magnitude when we control for labor input; (5) the IR between farm (and plot) size and total and family labor was negative and significant and the effect increases (in absolute terms) monotonically with quantiles.…”

Epigenetic changes in the early stage of silica-induced cell transformation by Carole Seidel (1854136)

“…Short-term treatment by Min-U-Sil^® 5 decreased global DNA methylation and increased the expression of the two <i>de novo</i> DNMTs, DNMT3a and DNMT3b. …”

4-methylthiobutyl isothiocyanate synergize the antiproliferative and pro-apoptotic effects of paclitaxel in human breast cancer cells by Harneetpal Kaur (13973217)

“…In western blot experiment, the combination had a significant decrease in Bcl-xl protein level, whereas a higher level of p53, cleaved caspase-3, and cleaved caspase-9 proteins compared to individual treatment in <i>T</i>-47D cells. …”

Altered Hematopoiesis in Mice Lacking DNA Polymerase μ Is Due to Inefficient Double-Strand Break Repair by Daniel Lucas (13351)

“…In Polμ^−/− mice, hematopoietic development was defective in several peripheral and bone marrow (BM) cell populations, with about a 40% decrease in BM cell number that affected several hematopoietic lineages. …”

Enzymatic activity and expression of HDACs in fPMSCs. by Yongzhao Zhu (691433)

“…Fold change in gene expression in P8 cells was normalized to donor-matched cells at P3. Comparisons of mean values between P3 and P8 cells were analyzed by using a student <i>t-test</i>. …”

Boyden chamber analysis of migration of parental and CR HT-29 cells. by Pratima Nangia-Makker (511071)

“…Top left panel: wound at 0 hr; bottom left panel: complete wound healing at 48 hr in CR HT-29 cells; top right panel: partial wound healing in HT-29 cells after 48 hr; bottom right panel: incomplete wound healing in CR HT-29 cells in the presence of 5 mM metformin/FuOx at 48 hr (B). Western blot analysis of metformin/FuOx treated CR H29 cells indicating decreased pAkt levels (C); relative changes in phospho-Akt (pAkt) were calculated as a ratio of pAkt/Akt after normalization to β-actin, which was used as loading control. …”

Inhibition of CFTR activity by fractions from Chinese wild grapevine. by Yaofang Zhang (112836)

“…<p>A. A representative plate with positive fractions in screening by cell-based fluorescent assay. …”

Illustration of the time course of the protocol by Qin Lu (995)

“…The lower panel represents the time course of the protocol for 7 patients for whom a CT scan and P–V curve in ZEEP were acquired after 15 minutes of mechanical ventilation without PEEP. …”

Localised Badger Culling Increases Risk of Herd Breakdown on Nearby, Not Focal, Land by Jon Bielby (70689)

“…We found that reactive badger culling had a significant positive association with the risk of cattle TB at distances of 1-3km and 3-5km, and that no such association existed over shorter distances (<1km). …”

Inhibition of Glioblastoma Growth by the Thiadiazolidinone Compound TDZD-8 by Diana Aguilar-Morante (237970)

“…These effects were associated with an early activation of extracellular signal-regulated kinase (ERK) pathway and increased expression of EGR-1 and p21 genes. Also, we observed a sustained activation of the ERK pathway, a concomitant phosphorylation and activation of ribosomal S6 kinase (p90RSK) and an inactivation of GSK-3β by phosphorylation at Ser 9. …”

Cellular automata to understand the prograding limit of deltaic tidal flat by Xuefei Mei (16541666)

“…Our results suggest that the tidal flat of NHS extended 29.16 m/y seaward in 1989–2002, had a rapid progradation of 158.08 m/y in 2002–2012 and a retreat rate of 26.88 m/y in 2012–2019, while the fluvial sediment supply decreased 56.78% from 1989–2002 to 2003–2012 and another 20.68% from 2003–2012 to 2013–2019. …”

Functional Implications of Novel Human Acid Sphingomyelinase Splice Variants by Cosima Rhein (168205)

“…The pathological effects of dysregulated ASM activity are evident in several human diseases and indicate an important functional role for ASM regulation. We investigated alternative splicing as a possible mechanism for regulating cellular ASM activity.…”

Data_Sheet_2_Rewiring of Gene Expression in Pseudomonas aeruginosa During Diauxic Growth Reveals an Indirect Regulation of the MexGHI-OpmD Efflux Pump by Hfq.pdf by Marlena Rozner (12911555)

“…As Hfq function is impeded by the RNA CrcZ upon relief of CCR, and Hfq is known to impact antibiotic susceptibility in P. aeruginosa, emphasis was laid on links between CCR and antibiotic susceptibility. To this end, we show that the mexGHI-opmD operon encoding an efflux pump for the antibiotic norfloxacin and the virulence factor 5-Methyl-phenazine is upregulated after alleviation of CCR, resulting in a decreased susceptibility to the antibiotic norfloxacin. …”

Data_Sheet_1_Rewiring of Gene Expression in Pseudomonas aeruginosa During Diauxic Growth Reveals an Indirect Regulation of the MexGHI-OpmD Efflux Pump by Hfq.xlsx by Marlena Rozner (12911555)

“…As Hfq function is impeded by the RNA CrcZ upon relief of CCR, and Hfq is known to impact antibiotic susceptibility in P. aeruginosa, emphasis was laid on links between CCR and antibiotic susceptibility. To this end, we show that the mexGHI-opmD operon encoding an efflux pump for the antibiotic norfloxacin and the virulence factor 5-Methyl-phenazine is upregulated after alleviation of CCR, resulting in a decreased susceptibility to the antibiotic norfloxacin. …”