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Proteasome activity was decreased in the <i>myc-rpt6-S119A</i> mutant.
Published 2017“…(C) The chymotrypsin-like activity of 26S proteasomes in whole cell lysates of stationary phase cells (A<sub>600</sub>>5.0) was monitored by Suc-LLVY-AMC cleavage over time on microplate reader (ex. 360nm and em. 465nm). The mean ± SEM rate of Suc-LLVY-AMC cleavage is decreased in the <i>myc-rpt6-S119A</i> mutant compared to the WT and <i>myc-rpt6-S119D</i> strains. …”
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MK-STYX decreases RhoA activation.
Published 2014“…<p>(<b>A</b>) PC12 cells were transfected with pMT2 or pMT2-FLAG-MK-STYX-FLAG. 24 hr post-transfection cells were stimulated with 100 ng/ml NGF, and lysed at the indicated time points. …”
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Decreased oscillatory flow decreases <i>klf2a</i> expression.
Published 2009“…(L) Quantitative RT-PCR showing the expression level of several flow-responsive genes after lidocaine treatment. <i>klf2a</i> expression is significantly decreased after 6 and 10 h of treatment and is restored by incubation at 34°C; 100% of expression corresponds to a normal expression level. …”
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