Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Experimental Analysis of Paraffin Deposition in Slug Flow by Aziz Alhejaili (10331604)

“…As the gas velocity increased, a slight increase in wax deposition was observed due to an increase in heat transfer, leading to a greater radial temperature gradient and, consequently, more wax deposition (∼5 g increase). …”

Transient Protection from Heat-Stress Induced Apoptotic Stimulation by Metastasis-Associated Protein 1 in Pachytene Spermatocytes by Wei Li (7081)

“…</p> <h3>Methodology/Principal Findings</h3><p>High level of MTA1 expression sustained for 1.5 h in primary spermatocytes after heat stress before a notable decrease was detected conversely correlated to the gradual increase of acetylation status of p53 and of p21 level. …”

Image_3_Experimental Myocardial Infarction Elicits Time-Dependent Patterns of Vascular Hypoxia in Peripheral Organs and in the Brain.TIF by Hélène David (8402883)

“…Acute LV dysfunction was associated with global hypoxia, specifically a decrease in sO₂ level in the brain (−5.9%), kidney (−6.4%), and liver (−7.3%) at 4 and 24 h post-MI. …”

Image_1_Combined Algorithm-Based Adaptations of Insulin Dose and Carbohydrate Intake During Exercise in Children With Type 1 Diabetes: Results From the CAR2DIAB Study.tif by Philippe Antoine Lysy (11334060)

“…Objectives<p>To evaluate the evolution of subcutaneous glucose during two sessions of monitored aerobic exercise in children or adolescents with type 1 diabetes after adaptation of insulin doses and carbohydrate intake according to a combined algorithm.</p>Methods<p>Twelve patients with type 1 diabetes (15.1 ± 2 years; diabetes duration: 9.5 ± 3.1 years) performed two series of exercise sessions after cardiac evaluation. …”

Supplementary Material for: Age-Related Association of Calcitonin with Parameters of Anthropometry, Bone and Calcium Metabolism during Childhood by Sonntag J. (9759320)

“…<b><i>Introduction:</i></b> The thyroid parafollicular hormone calcitonin (CT) shows particularly high blood levels in early childhood, a period of high bone turnover, which decrease with increasing age. …”

Increase in Female Liver Cancer in The Gambia, West Africa: Evidence from 19 Years of Population-Based Cancer Registration (1988–2006) by Dominique Sighoko (225218)

“…Male to female ratio showed a significant decrease between the two periods from 3.28∶1 (95% CI, [2.93–3.65]) to 2.2∶1 (95% CI, [1.99–2.43]). …”

Data_Sheet_1_Metagenomic Investigation Uncovers Presence of Probiotic-Type Microbiome in Kalparasa® (Fresh Unfermented Coconut Inflorescence Sap).docx by Murali Gopal (29502)

“…The 12-h fermented Kalparasa showed a significant increase in Gluconobacter with a sharp decrease in Leuconostoc. …”

Accumulation of HPV23 E6 protein is caused by HIPK2. by Dorothea Muschik (196318)

“…Reduction of HIPK2 protein caused a decrease of E6 protein levels shown by Western blot analysis. …”

Data_Sheet_1_Identification of RNAi hypoallergic bread wheat lines for wheat-dependent exercise-induced anaphylaxis patients.docx by María H. Guzmán-López (17791796)

“…In all cases, the reduction in gliadin content correlated with a decrease in IgE reactivity observed in the sera of WDEIA patients, highlighting the E82 and H320 lines. …”

Trade-Off for Better Balanced Nonlinear Optical Performance with Disordered Si in ZnGeP₂ by Shunda Yang (8086265)

“…ZnGeP₂ (ZGP) possesses prominent mid-far infrared nonlinear optical (NLO) performance but suffers from a narrow bandgap. A comparative analysis revealed that the bandgap could be enhanced by substituting Si for Ge in ZGP, successfully synthesizing a series of ZnSi_{<b><i>x</i></b>}Ge_{1–<b><i>x</i></b>}P₂ crystals with enlarged bandgaps compared with that of ZGP. …”

Image_2_Experimental Myocardial Infarction Elicits Time-Dependent Patterns of Vascular Hypoxia in Peripheral Organs and in the Brain.TIF by Hélène David (8402883)

“…Acute LV dysfunction was associated with global hypoxia, specifically a decrease in sO₂ level in the brain (−5.9%), kidney (−6.4%), and liver (−7.3%) at 4 and 24 h post-MI. …”

Trade-Off for Better Balanced Nonlinear Optical Performance with Disordered Si in ZnGeP₂ by Shunda Yang (8086265)

“…ZnGeP₂ (ZGP) possesses prominent mid-far infrared nonlinear optical (NLO) performance but suffers from a narrow bandgap. A comparative analysis revealed that the bandgap could be enhanced by substituting Si for Ge in ZGP, successfully synthesizing a series of ZnSi_{<b><i>x</i></b>}Ge_{1–<b><i>x</i></b>}P₂ crystals with enlarged bandgaps compared with that of ZGP. …”

Autophagy Interplay with Apoptosis and Cell Cycle Regulation in the Growth Inhibiting Effect of Resveratrol in Glioma Cells by Eduardo C. Filippi-Chiela (144739)

“…While inhibition of apoptosis or autophagy alone did not revert Rsv-induced toxicity, inhibition of both processes blocked this toxicity. Rsv also induced a S-G2/M phase arrest, accompanied by an increase on levels of pCdc2(Y15), cyclin A, E and B, and pRb (S807/811) and a decrease of cyclin D1. …”

Supercooled Water Drops Impacting Superhydrophobic Textures by Tanmoy Maitra (1606972)

“…Viscous effects in water supercooling conditions cause a reduction of drop maximum spreading (∼25% at an impact speed of 3 m/s for a millimetric drop) and can significantly decrease the drop recoil speed when the meniscus partially penetrates into the texture, leading to an increase of the contact time up to a factor of 2 in supercooling conditions compared to room temperature. …”

Experimental Analysis of Paraffin Deposition in Slug Flow by Aziz Alhejaili (10331604)

“…As the gas velocity increased, a slight increase in wax deposition was observed due to an increase in heat transfer, leading to a greater radial temperature gradient and, consequently, more wax deposition (∼5 g increase). …”

Image_4_Experimental Myocardial Infarction Elicits Time-Dependent Patterns of Vascular Hypoxia in Peripheral Organs and in the Brain.TIF by Hélène David (8402883)

“…Acute LV dysfunction was associated with global hypoxia, specifically a decrease in sO₂ level in the brain (−5.9%), kidney (−6.4%), and liver (−7.3%) at 4 and 24 h post-MI. …”

Arrest in G1 and transcriptional analysis of <i>Δrps13</i> ±ATc. by Samuel L. Hutson (236870)

“…<p>(<b>A</b>) G1 arrest shown by FACS. There is an increase of 1N and decrease of 1.8 to 2N population at 24 hours -ATc, a profile seen at the G1 phase of synchronously growing parasites. …”