Flow cytometry data - Computationally Engineered CRISPR-SpyCas9 High-Fidelity Variants with Improved Specificity and Reduced Non-specific DNA Damage by Roy Rabinowitz (16908351)

“…<p>EGFP-PEST stable cells were co-transfected with one of the sgRNA plasmids and one of the Cas plasmids, together with a reporter plasmid. Five days post transfection cells were trypsinized, incubated 5 min at 37 °C, and neutralized by 150ul FBS-enriched FACS buffer (PBS; 5% FBS; 25mM HEPES Biological industries (Israel) 03-025; 5mM EDTA, Biological industries (Israel) 01-862). …”

TGM2 inhibition by siRNA treatment induces cell apoptosis. by Yin-Cheng Huang (633119)

“…Meningioma cells treated with <i>TGM2</i> siRNA exhibited reduced TGM2 expression in a dose-dependent manner (>80% in 5 nM siRNA). (B) Cell viability assessed using the MTT assay showed 30% decrease of cell survival following 5 nM siRNA exposure. …”

Lpp supplies lipids to peripheral organs. by Wilhelm Palm (148641)

“…<p>(A–D) Changes in wing disc neutral lipids upon LTP or Lpp RNAi, quantified by mass spectrometry (n = 5). …”

MMP-2, MMP-9, TIMP-3 expression and collagenolytic activity in the failing heart. by Federica Limana (216501)

“…(E) TIMP-3 protein levels were lower in failing hearts, both in the border zone and in the infarcted area, than in SO hearts. HMGB1 caused a further decrease in TIMP-3 protein. Both for MMPs (C) and TIMP-3, the same filter was probed with α-tubulin to normalize protein loading (n = 5 for the average results of densitometric analyses of western blots).…”

Transmission kernels. by Elisa Benincà (120328)

“…Parameter values are based on estimates by Boender et al. [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1008009#pcbi.1008009.ref005" target="_blank">5</a>] for the Dutch epidemic of avian influenza in 2003, but with rescaling to account for the decrease in number of farms (from 5,360 to 2,157) and concomitant increase in size of farms since 2003.…”

Table_1_Correlation analysis between 18F-fluorodeoxyglucose positron emission tomography and cognitive function in first diagnosed Parkinson’s disease patients.docx by Sun Zhihui (16320159)

“…Further regression analysis showed that for every one-point decrease in the memory score, the glucose metabolism in the right precuneus would decrease by 0.3 (B = 0.30, p = 0.005), the glucose metabolism in the left primary visual cortex would decrease by 0.25 (B = 0.25, p = 0.040), the glucose metabolism in the right lateral occipital cortex would decrease by 0.38 (B = 0.38, p = 0.012), and the glucose metabolism in the left lateral occipital cortex would decrease by 0.32 (B = 0.32, p = 0.045).…”

Table_2_Correlation analysis between 18F-fluorodeoxyglucose positron emission tomography and cognitive function in first diagnosed Parkinson’s disease patients.docx by Sun Zhihui (16320159)

“…Further regression analysis showed that for every one-point decrease in the memory score, the glucose metabolism in the right precuneus would decrease by 0.3 (B = 0.30, p = 0.005), the glucose metabolism in the left primary visual cortex would decrease by 0.25 (B = 0.25, p = 0.040), the glucose metabolism in the right lateral occipital cortex would decrease by 0.38 (B = 0.38, p = 0.012), and the glucose metabolism in the left lateral occipital cortex would decrease by 0.32 (B = 0.32, p = 0.045).…”

DataSheet1_Electrokinetic properties of healthy and β-thalassemia erythrocyte membranes under in vitro exposure to static magnetic field.pdf by Virjinia Doltchinkova (17203663)

“…</p><p>Results: The electrokinetic potential of erythrocytes in norm is increased after SMF intensities due to enhanced negatively exposed charges on the outer surface of the membrane accompanied by an increase in light scattering where changes in cell morphology are observed. Conversely, a decrease in the zeta potential of β-thalassemia erythrocytes upon SMF-treatment was determined because of the reduction in the surface electrical charge of the membranes, where a significant decrease in light scattering at 1.5 T and 2.0 T was recorded. …”

Characterization of super carbonate apatite (sCA). by Xin Wu (10164)

“…(C) Zeta potential (mV) of sCA (left) and sCA+0.5% albumin (right) were found to be-10.69 ± 2.19 mV and-16.02 ± 0.89 mV, (mean ± SD; n = 5), when incubated by 4mM Ca²⁺-supplemented bicarbonated buffer. …”

Table 1_Gender difference in the association between composite dietary antioxidant index and all-cause mortality.docx by Lanzhi Duan (15350413)

“…</p>Results<p>The study encompassed a sample of 15,651 individuals. The mean CDAI was 0.52 ± 6.06. …”

Investigating the abundance of herbaceous plant species in an area dominated by woody plant species by Balsharn Heir (3113820)

“…Quadrats (0.5 x 0.5m) were randomly placed every two meters, alternating left and right, along a 50m transect. …”

Microarray detection of <i>NLRPs</i> in human oocytes and embryos showed two different expression patterns. by Pu Zhang (51027)

“…<p>A: <i>NLRP4, 5, 8, 9, 11,12, 13 and14</i> were highly expressed in oocytes and then gradually decreased in embryos with a very low level in day5 (D5) embryos. …”

Survival of Campylobacter jejuni in chicken at refrigeration and cooking temperatures by Clarissa Silveira Luiz Vaz (12701386)

Supplementary data from Temperature preferences drive additive biotic homogenization of Orthoptera assemblages by Simon Thorn (12492143)

SOIL PHYSICAL ATTRIBUTES BEFORE AND AFTER MANAGEMENT UNDER SOWING FORWARD SPEED by Jorge W. Cortez (5312624)

“…The medium harrowing operation has higher sD and the ideal forward sowing speed range to mitigate this effect would be 5.0 to 7.0 km h−1. Moreover, it also presents a higher amount of macropores in the 0.20–0.30 m layer. …”

Clinical improvement of juvenile macaques with chronic diarrhea following <i>T. trichiura</i> treatment. by Mara Jana Broadhurst (120227)

“…<p>(<b>A</b>) Diarrhea of five macaques was scored daily before and after oral administration of germinated <i>T. trichiura</i> (Tt) ova. …”

Effect of reducing stress on biofilm. by Naeem Anwar (418076)

“…Coomassie blue stained SDS-PAGE (<b>B</b>) and immunoblot (<b>C</b>) show decrease in the amounts of respectively CsgA and CsgD under 5 mM DTT stress from 18 hours at 28°C. …”

Small molecular chaperone 4-phenylbutyric acid (PBA) rescues APP proteolysis from thapsigargin and tunicamycin treatment. by Jesse C. Wiley (234079)

“…<p>NAG cells either remained untreated, or were treated with thapsigargin (0.25 µg/ml), tunicamycin (5 µg/ml), or brefeldin A (5 µg/ml) alone, or in conjunction with titrated values of PBA, for 24 hours. …”

Effects of intragastric infusion of Olanzapine on liver insulin sensitivity (Experiment 2). by Elodie M. Girault (144494)

“…<p>(Vehicle group n = 8, Olanzapine group n = 7) 4a: Endogenous glucose production at basal (mean of 3 time points: t = 90 to t = 100; white bars) and during the hyperinsulinemic state (mean of 5 time points: t = 250 to t = 290; black bars). …”

Sex differences in triglyceride storage and breakdown are not due to male and female gonads. by Lianna W. Wat (8339454)

“…(G) In 5-day-old virgin female <i>w</i>^<i>1118</i> carcasses devoid of ovaries, there was no significant decrease in triglyceride levels between 0 and 12 hours STV, whereas there was a significant reduction in triglyceride levels in age-matched <i>w</i>^<i>1118</i> virgin male carcasses devoid of testes (<i>p</i> = 0.15 and 0.013, respectively; one-way ANOVA followed by Tukey HSD test). …”