Ras Tyrosine 4 is required for Rabex-5-mediated Ras ubiquitination. by Chalita Washington (8997378)

“…<p>(A) Flag-His6-GFP tagged Ras (Ras^WT) or FLAG-His6-GFP tagged Ras N-terminal fragments tagged with C-terminal localization signal CKML (1–10 CKML, 1–20 CKML) were co-transfected into Schneider S2 cells with HA-Ub with or without Rabex-5 and purified on nickel beads as done previously [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008715#pgen.1008715.ref013" target="_blank">13</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1008715#pgen.1008715.ref016" target="_blank">16</a>]. …”

Image 1_Investigating the potential immunomodulatory effects of commercial oral probiotic supplements on equine gastrointestinal tract barrier function.jpeg by Agnieszka Żak-Bochenek (20588453)

“…The samples were subjected to microbiome analysis via next-generation sequencing of hypervariable regions V3-V4 and V7-V9 of the 16S rRNA gene for analysis of short-chain fatty acids via HPLC analysis and fecal secretory immunoglobulin A (SIgA) quantification via ELISA.…”

Table 1_Investigating the potential immunomodulatory effects of commercial oral probiotic supplements on equine gastrointestinal tract barrier function.docx by Agnieszka Żak-Bochenek (20588453)

“…The samples were subjected to microbiome analysis via next-generation sequencing of hypervariable regions V3-V4 and V7-V9 of the 16S rRNA gene for analysis of short-chain fatty acids via HPLC analysis and fecal secretory immunoglobulin A (SIgA) quantification via ELISA.…”

S2 Fig - by Ty A. Bottorff (14035065)

Evaluation of the potential of Rejuveinix plus dexamethasone against sepsis - Fig S5 by Fatih M Uckun (13809802)

Silencing of the reporter gene <i>URA5-miRs</i>. by Nan Jiang (21252)

“…<p>(A) The upper panels show a slower growth rate of the transformants of <i>URA5-miR1</i> (two transformants was picked in each case, namely, miR1-1 and miR1-2), and <i>URA5-miR2</i> (miR2-1 and miR2-2), than the wild type B4500, and the transformants of miR1-m (<i>i.e.…”

Altered regulation of Prox1-gene-expression in liver tumors-5 by Jozsef Dudas (46097)

“…When the nuclear extracts were reacted with the same radiolabelled consensus double-stranded oligo in the presence of a 100-fold excess of an unlabeled MAZ-consensus oligo (published in ref. [23]) the intensity of mobility shift significantly decreased (right panel); sample 1: negative control, without nuclear extract. …”

Nickel Complexes Bearing ONS Chelating Ligands: A Promising Contender for In Vitro Cytotoxicity Effects on Human Pancreatic Cancer MIA-PaCa‑2 Cells by Deepika Tanwar (14329312)

“…Hence, in this study, we synthesized and characterized a series of ONS donor ligands [2-HO–C₆H₄–CHN–(C₆H₄)–SH] (<b>L1</b>), [2-OH-3-OMe–C₆H₃–CHN–(C₆H₄)–SH] (<b>L2</b>), [2-OH-3,5-(C(Me)₃)₂–C₆H₂–CHN–(C₆H₄)–SH] (<b>L3</b>), [2-OH–C₆H₄–CHN–(C₆H₄)–SMe] (<b>L4</b>), [2-OH-3-OMe–C₆H₃–CHN–(C₆H₄)–SMe] (<b>L5</b>), [2-OH-3,5-(C(Me)₃)₂–C₆H₂–CHN–(C₆H₄)–SMe] (<b>L6</b>) and their Ni(II) metal complexes [(MeOH)Ni(<b>L1–L1–</b>4H)] (<b>1</b>), [(MeOH)Ni(<b>L2–L2–</b>4H)] (<b>2</b>), [(MeOH)Ni(<b>L3–L3–</b>4H)] (<b>3</b>), [(<b>L4–</b>H)₂Ni] (<b>4</b>), [(<b>L5–</b>H)₂Ni] (<b>5</b>), and [(<b>L6–</b>H)₂Ni] (<b>6</b>). …”

Increased 5-hmC occurs in osteoblast and chondrocyte gene promoters. by Richard C. Lindsey (7148168)

“…(B) Inhibition of PHD2 with Iox2 significantly decreased 5-hmC levels in the promoters of chondrocyte genes HIF1α and VEGF. …”

UHRF2 regulates local 5-methylcytosine and suppresses spontaneous seizures by Yidan Liu (3876598)

“…Therefore, our study has revealed a unique role of UHRF2 in the maintenance of local 5mC levels in brain that is distinct from that of its paralog UHRF1.…”

Reduced control model M5: No accumulation of information across trials. by Veith Weilnhammer (2909060)

“…<p>When simulating data for the <i>no-evidence-accumulation model</i>, we removed the accumulation of information across trials by setting the hazard rate <i>H</i> to 0.5. Simulated data thus depended only on the participant-wise estimates for the amplitudes <i>a</i>_{<i>LLR</i>/<i>ψ</i>}, frequency <i>f</i>, phase <i>p</i>, and inverse decision temperature <i>ζ</i>. …”

Image_5_Climatic and Resource Determinants of Forest Elephant Movements.pdf by Christopher Beirne (636588)

Table_5_Clostridium cellulovorans Proteomic Responses to Butanol Stress.XLSX by Paolo Costa (1396552)

“…Several additional metabolic adaptations were triggered by butanol exposure such as the up-regulation of V- and F-type ATPases (involved in ATP synthesis/generation of proton motive force), enzymes involved in amino acid (e.g., arginine, lysine, methionine, and branched chain amino acids) biosynthesis and proteins involved in cell envelope re-arrangement (e.g., the products of Clocel_4136, Clocel_4137, Clocel_4144, Clocel_4162 and Clocel_4352, involved in the biosynthesis of saturated fatty acids) and a redistribution of carbon flux through fermentative pathways (acetate and formate yields were increased and decreased, respectively). …”

Influenza-induced loss of claudin-5 is due to cleavage by matrix metalloproteases. by Susan M. Armstrong (301641)

“…<p>(A) The reduction in claudin-5 levels is not due to decreased transcription. …”

Receptors mediated changes in ERK1/2 and ERK5 phosphorylation. by Rishi K. Somvanshi (506704)

“…Note that the combined agonist treatment had pronounced effect on ERK5 phosphorylation. Densitometric analysis for the blots (in Panels A-D) was performed by using β-actin or total as loading control and data analysis was done by using ANOVA and <i>post hoc</i> Dunnett’s to compare against basal level (*, <i>p</i><0.05).…”

Video_5_Rora Regulates Neutrophil Migration and Activation in Zebrafish.avi by Alan Y. Hsu (5037197)

Table_1_Interpopulation variation in seed traits of five Polygonaceae.docx by Arvind Bhatt (14825233)

“…<p>Interpopulation variability in seed traits may drive the regeneration capacity of a species to colonize different environments. In the present study, we evaluated the variation in seed physical traits (mass / size, water imbibition, shape index) and physiological traits (germination) of five Polygonaceae species. …”

SARS-CoV-2 proximal proteins in translation and interferon activation. by Jordan M. Meyers (3685879)

Figure S1-S21 from miR-196b-5p Regulates Colorectal Cancer Cell Migration and Metastases through Interaction with HOXB7 and GALNT5 by Verena Stiegelbauer (4582930)

“…<p>Supplementary Figure S1 Expression levels of miR-196b-5p in different tumor stages and tumor grade. (A-B) In cohort 1, miR-196b-5p expression decreased with (A) higher tumor stage, whereas no difference could be observed for (B) tumor grade. …”

The miRNA gene clusters and their genomic structures of cluster #5∼cluster #8, which are part of 11 miRNA gene clusters shown in figure 5, 6 and 7. by Xiaomin Zhang (136250)

“…Please note that part of the miRNAs with changes in expression greater than 1.5 fold in old (O) vs. young adult (YA) hearts were listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034688#pone-0034688-t002" target="_blank">table 2</a>.…”