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    Table1_A depth-first search algorithm for oligonucleotide design in gene assembly.DOCX by Hanjie Liang (12253847)

    Published 2022
    “…This will decrease the errors in gene assembly with PCR or LCR. Lastly, the oligonucleotides that have homologous melting temperatures needed for PCR-based synthesis and two-step assembly of the target gene are deduced and outputted.…”