يعرض 41 - 60 نتائج من 24,968 نتيجة بحث عن '(( c values increased ) OR ( ct larger decrease ))', وقت الاستعلام: 0.51s تنقيح النتائج
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    Increasing the amount of surface-bound C5b-8 impairs polymerization of C9. حسب Dennis J. Doorduijn (9010241)

    منشور في 2021
    "…Bacteria were washed and next incubated with a concentration range of C8 and 3 nM (a,b,d) or 1 nM (c) Cy5-labelled C9<sub>wt</sub> or C9<sub>TMH-1 lock</sub> for 30 minutes. a) Binding of Cy5-labelled C9<sub>wt</sub> or C9<sub>TMH-1 lock</sub> to bacteria measured by flow cytometry. b) The relative increase in C9<sub>wt</sub> binding compared to bacteria labelled with C9<sub>TMH-1 lock</sub> was calculated as indication for C9 polymerization. c) periplasmic mCherry (<sub>peri</sub>mCherry) leakage was measured after 30 minutes by flow cytometry and represented as relative <sub>peri</sub>mCherry fluorescence compared to t = 0. d) Bacterial cell pellets were analyzed by SDS-PAGE for in-gel fluorescence of Cy5-labelled C9<sub>wt</sub> to distinguish monomeric-C9 from polymeric-C9. …"
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