Development of a CRISPR/Cas-Based Detection Platform for Tracking Decreased Susceptibility to Cephalosporins in Neisseria gonorrheae by Ziyuan Zhao (2889005)

“…Developing efficient and accurate detection of resistant strains is urgently needed for their management and treatment. We have developed the Multiplex Integrated RPA-CRISPR/Cas12a detection Assay (MIRCA) for simultaneous detection of Neisseria gonorrheae (<i>Ng</i>) and mutations with decreased susceptibility to cephalosporins. …”

CRISPR/RfxCas13d guide RNAs used in this study. by Mitchell Hayes (5334887)

Table 1_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.xlsx by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Image 4_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.jpeg by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Image 5_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.jpeg by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Image 1_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.jpeg by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Image 3_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.jpeg by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Image 6_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.jpeg by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Image 2_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.jpeg by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Data Sheet 1_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.docx by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

Supplementary file 1_CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.docx by Hyungjun Park (14580903)

“…However, its impact on agricultural traits has remained elusive.</p>Methods<p>We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.…”

AI KRegression by CHIN-CHENG CHU (20718575)

Identification of the F’ composition by LC-MS/MS. by Baoyin Chen (2635399)

“…However, its full function remains to be addressed. We created two <i>c1-fdx</i> mutants in Arabidopsis using the CRISPR-Cas9 methodology. …”

Sequence alignment of Arabidopsis ACP1 and ACP2. by Baoyin Chen (2635399)

“…However, its full function remains to be addressed. We created two <i>c1-fdx</i> mutants in Arabidopsis using the CRISPR-Cas9 methodology. …”

A novel RNN architecture to improve the precision of ship trajectory predictions by Martha Dais Ferreira (18704596)

“…To solve these challenges, Recurrent Neural Network (RNN) models have been applied to STP to allow scalability for large data sets and to capture larger regions or anomalous vessels behavior. This research proposes a new RNN architecture that decreases the prediction error up to 50% for cargo vessels when compared to the OU model. …”

BAR-CAT: Targeted Recovery of Synthetic Genes via Barcode-Directed CRISPR-dCas9 Enrichment by Natanya Villegas (20912585)

“…To rescue these rare, error-free molecules we developed BAR-CAT (Barcode-Assisted Retrieval CRISPR-Activated Targeting), an in-vitro enrichment method that couples unique PAM-adjacent 20-nt barcodes to each library member and uses multiplexed dCas9-sgRNA complexes to fish out the barcodes corresponding to perfect assemblies. …”

Image 1_A rapid and naked-eye methicillin resistant Staphylococcus aureus screening method based on CRISPR/Cas12a and hybridization chain reaction.tif by Yayun Jiang (11556973)

“…In the presence of the DNA targets, the trans-cleavage activity of CRISPR/Cas12a is activated to cleave the trigger strand, failing HCR and leading to a decrease in the fluorescence signal. …”

Supplementary file 1_A rapid and naked-eye methicillin resistant Staphylococcus aureus screening method based on CRISPR/Cas12a and hybridization chain reaction.docx by Yayun Jiang (11556973)

“…In the presence of the DNA targets, the trans-cleavage activity of CRISPR/Cas12a is activated to cleave the trigger strand, failing HCR and leading to a decrease in the fluorescence signal. …”

Image 1_PD-L1 knockout or ZG16 overexpression inhibits PDAC progression and modulates TAM polarization.jpeg by Hui Meng (197494)

“…Both methods promoted the polarization of tumor-associated macrophages (TAMs) to the M1 phenotype, as indicated by increased levels of the M1 marker CD11c+ in vitro and in vivo. Meanwhile, we observed a reduction in the M2 marker CD206+, upregulation of immune activation-related cytokines/chemokines, and a decrease in immunosuppressive cytokines and tumor angiogenesis factors. …”

CRISPR/Cas9-mediated editing of barley lipoxygenase genes promotes grain fatty acid accumulation and storability by Zhanghui Zeng (3571331)

“…Although the genetic engineering of <i>LOXs</i> holds great potential for improving grain storage quality, this approach remains largely unexplored in barley. In this study, we identified five <i>LOX</i> genes in the barley genome: <i>HvLOXA</i>, <i>HvLOXB</i>, and <i>HvLOXC1–3</i>. …”