The impact of excessive DNA amounts used for bisulfite conversion on the amplification efficiency and the methylation level of <i>LINE-1</i> and <i>Alu</i>. by Trang Thi Quynh Tran (20485595)

“…A serial dilution of human methylated DNA amounts, corresponding to 500 ng, 50 ng, 5 ng, 1 ng and 0.5 ng was treated by bisulfite. The ΔCT values significantly differ at input amounts from 500 ng to 0.5 ng. …”

Assessment of the relationship between adiposity, Sirtuin 1, and miR-30a-5p in a mixed-ancestry population in Bellville South, Cape Town. by Babalwa Gaxamba (20810513)

“…Key variables include sample IDs, absorbance values, cycle threshold (CT) values, ΔCT, 2^-ΔCT, ΔΔCT, and 2^-ΔΔCT calculations. …”

Image 2_Phage therapy for extensively drug resistant Acinetobacter baumannii infection: case report and in vivo evaluation of the distribution of phage and the impact on gut microb... by Jiayao Qu (11309880)

“…In fecal samples, a significant reduction in the Ct value of phages was observed following both phases of bacteriophage treatment, with values decreasing from 35.5 to 22.5 and from 32.6 to 22.7, respectively. …”

Image 1_Phage therapy for extensively drug resistant Acinetobacter baumannii infection: case report and in vivo evaluation of the distribution of phage and the impact on gut microb... by Jiayao Qu (11309880)

“…In fecal samples, a significant reduction in the Ct value of phages was observed following both phases of bacteriophage treatment, with values decreasing from 35.5 to 22.5 and from 32.6 to 22.7, respectively. …”

Data. by Su Hwan Park (15158181)

“…<div><p>Purpose</p><p>To evaluate idiopathic epiretinal membrane (iERM) area changes and the association with visual function in patients with good vision, using confocal scanning laser ophthalmoscopy (cSLO).</p><p>Methods</p><p>The medical records of patients with iERM with best-corrected visual acuity (BCVA) ≥ 20/25 and a follow-up period ≥ 2 years were reviewed retrospectively. …”

Increased selectivity requires competition and nonlinear activation function in pyramidal cells. by Lilia Kukovska (22683586)

“…Note that no-go selectivity was multiplied by −1 to indicate increased selectivity if above 0 and decreased selectivity if below 0. <b>D)</b> The difference in absolute selectivity between laser on and laser off conditions for ‘Entire’ and ‘Early’ PV cell activation in a model with weaker competition. …”

Evaluation of PCR amplification bias and PCR amplification efficiency. by Trang Thi Quynh Tran (20485595)

“…(<b>C</b>) The ΔCt value, calculated by subtracting the CT value of the MSP primer set from that of the MIP primer set for each sample, proportionally increases as the DNA input decreases, indicating the Pfaffl formula to be suitable for relative quantification. …”

Baseline characteristics of patients. by Su Hwan Park (15158181)

“…<div><p>Purpose</p><p>To evaluate idiopathic epiretinal membrane (iERM) area changes and the association with visual function in patients with good vision, using confocal scanning laser ophthalmoscopy (cSLO).</p><p>Methods</p><p>The medical records of patients with iERM with best-corrected visual acuity (BCVA) ≥ 20/25 and a follow-up period ≥ 2 years were reviewed retrospectively. …”

Calcium imaging reveals comparable calcium responses and contractions in rat and human sweat glands. by Teresa Franziska Buck (21272954)

“…(B) Cholinergic stimulation of sweat glands with mACh induces a sharp Fura Red™ (red) decrease and Fluo-4 (green) increase in both species, indicating a strong intracellular calcium rise (exemplary depiction). …”

Table 1_Study on the antibacterial effect of the new anti-biofilm inhibitor ICAC on Escherichia coli.docx by Yubin Bai (1350858)

“…The study utilized a comprehensive approach encompassing crystal violet staining, scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), the ruthenium red method, semi-solid agar motility assays, and quantitative real-time PCR (qRT-PCR) to elucidate the inhibitory effects and their underlying mechanisms. …”

Impact of pathogen shedding characteristics on estimation accuracy. by Yun Lin (77976)

“…The violin plot represents the distribution of the detected Ct values for the 200 randomly selected individuals in each trajectory panel, and the horizontal dashed line represents the median Ct value at detection for individuals infected with each pathogen during the increasing epidemic as a reference for comparing the Ct distributions for each pathogen during different epidemic periods. …”

(related to Fig 5) Effects of PV cell activation on the activity of Pyr cells across the different time windows and task difficulty conditions. by Lilia Kukovska (22683586)

“…Color bar range indicates activity, with positive values (blue) for cells increasing their activity and negative values (red) for cells decreasing their activity as a result of laser stimulation. …”

Data Sheet 1_Amyloid-induced mitochondrial network disruption in neurons monitored by STED super-resolution imaging.pdf by Iuliia Golovynska (18880049)

“…</p>Methods<p>A detailed analysis of mitochondrial network in healthy neurons and those exposed to Aβ is performed using STED compared to conventional laser-scanning confocal fluorescence microscopy. …”

Image 1_The microbiome exists in the neuroretina and choroid in normal conditions and responds rapidly to retinal injury.tif by Xuexue Cui (11574637)

“…</p>Methods<p>Adult C57BL/6J mice were subjected to retinal laser burns using a photocoagulator. One and 24h later, the retina and RPE/choroid were collected under strict sterile conditions and processed for 16S rRNA paired-end sequencing (2×250). …”

Time Since Deposition Signatures for the Analysis of Canine Body Fluid by Alysia Townsley (20673209)

“…Autofluorescence profiles of individual cells were characterized using a 488nm excitation laser and detector wavelengths between 535nm-700nm. …”

Table 1_Negative pressure breathing: the response of human respiration and circulation to different levels of rarefaction during inspiration.docx by Yury S. Semenov (20345100)

“…Introduction<p>Negative pressure breathing is breathing with decreased pressure in the respiratory tract without lowering pressure acting on the torso. …”

Ambulatory modeling of vocal hyperfunction (Parra et al., 2025) by Jesús A. Parra (20677929)

“…</p><p dir="ltr"><b>Results: </b>Results showed significant differences between the PVH and control groups in key parameters, including statistical moments of H1–H2, SPL, PS, and muscle activity of lateral cricoarytenoid (LCA) and cricothyroid (CT) muscles. Higher DPI values, reflecting more severe PVH, were associated with increased mean LCA activation and decreased LCA variability, along with decreased mean CT activation and increased median PS. …”

Data_Sheet_1_Antibacterial activity and mechanism of Stevia extract against antibiotic-resistant Escherichia coli by interfering with the permeability of the cell wall and the memb... by Xu Chen (432759)

“…The uptake of propidium iodide (PI), a compromised cell membrane nucleic acid stain, and confocal laser scanning microscopy (CLSM) further demonstrated that ICAC disrupted IM integrity. …”

Spectral shift of fluorophore NHS-esters. by Hyunwoo Kim (690467)

“…After 5 min of illumination with a 561-nm excitation laser, the fluorescence signal in the 647-nm detection channel decreased, while the signal in the 561-nm detection channel significantly increased, indicating a spectral blue-shift of the emission spectrum. …”

Data Sheet 1_Cytokine-driven glycosphingolipid metabolism modulates endoplasmic reticulum calcium homeostasis in primary human renal mesangial cells.pdf by Mariia Stefanenko (16987420)

“…The increase in cytosolic Ca²⁺ was attributed to a decrease in endoplasmic reticulum (ER) Ca²⁺ store capacity. …”