Aberrant CpG methylation of the <i>TFAP2A</i> gene constitutes a mechanism for loss of <i>TFAP2A</i> expression in human metastatic melanoma by Andrea R Hallberg (686570)

Image_4_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Image_3_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Image_2_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Image_1_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.TIF by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Table_1_Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups.DOC by Jie-Jie Shen (5600129)

“…In Streptomycestsukubaensis YN06, we found that AT4_FkbB [an AT in the fourth module of tacrolimus (FK506) PKS] transferred both allylmalonyl (allmal) and emthylmalonyl (ethmal) units to ACPs, which was supposed responsible for the production of both FK506 and its analog FK520, respectively. Mutations of five residues in AT4_FkbB (Q119A, L185I-V186D-V187T, and F203L) caused decreased efficiency of allmal transfer, but a higher ratio of ethmal transfer, supposedly due to less nucleophilic attacks between Ser599 in the active site of AT4_FkbB and the carbonyl carbon in the allmal unit, as observed from molecular dynamics simulations. …”

Do ecological characteristics drive the prevalence of <i>Panulirus argus</i> virus 1 (PaV1) in juvenile Caribbean spiny lobsters in a tropical reef lagoon? by Charlotte E. Davies (8506347)

“…<div><p>PaV1 is a pathogenic virus found only to infect Caribbean spiny lobsters <i>Panulirus argus</i>, a major fishing resource. …”

The impact of chronic kidney disease on developed countries from a health economics perspective: A systematic scoping review by Sarah Elshahat (8587107)

“…Mean EuroQol-5D index scores ranged from 0.80 to 0.86 for CKD stages 1–3, and decreased to 0.73–0.79 for CKD stages 4–5. …”

Number of bars of length greater than <i>ℓ</i> = 4000 in the barcodes obtained via the binary model (left) and the fuzzy binary model (right) as a function of <i>τ</i>. by Samir Chowdhury (5715338)

Data_Sheet_1_Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA.DOCX by Yuri Furusawa (5546819)

Evaporation of Dilute Sodium Dodecyl Sulfate Droplets on a Hydrophobic Substrate by Wojciech Kwieciński (7037669)

“…In contrast to the constant contact angle of an evaporating sessile water droplet, we observed that, at the same surface, the contact angle of an SDS laden droplet with concentration below 0.5 CMC first decreases, then increases, and finally decreases, resulting in a local contact angle minimum. …”

<i>PPARD</i> G32E mediates down-regulation of critical genes in the Wnt/<i>β-catenin</i> signaling pathway. by Jun Ren (110002)

“…<p>(A) Overexpression of mutant PPARD decreases TCF/LEF reporter activity. …”

Data_Sheet_2_Psychometric validation of a brief self-report measure of misophonia symptoms and functional impairment: The duke-vanderbilt misophonia screening questionnaire.pdf by Zachary J. Williams (8679021)

Data_Sheet_1_Psychometric validation of a brief self-report measure of misophonia symptoms and functional impairment: The duke-vanderbilt misophonia screening questionnaire.docx by Zachary J. Williams (8679021)

Structure–Activity Relationships and Discovery of (<i>S</i>)‑5‑(<i>tert</i>-Butyl)-11-(difluoromethoxy)-9-methoxy-2-oxo-1,2,5,6-tetrahydropyrido[2′,1′:2,3]imidazo[4,5‑<i>h</i>]quin... by Dimitar Gotchev (1893928)

“…Optimization of a novel series of PAPD5/7 inhibitors that decrease HBsAg levels led to the tetracyclic 2-pyridone <b>AB-161</b>, which was similarly potent to <b>AB-452</b> <i>in vitro</i> and <i>in vivo</i> but showed dramatically higher rodent liver-to-plasma ratios. …”

Number of samples for which a site is covered across for datasets generated using (A) RRBS and (B) TMS. by Amy Longtin (21409957)

Stay duration (means ± SEM, in minutes) in quinpirole-treated and in saline-treated rats exposed first to elastic and then to symmetrical object-setting (a) and rats exposed first... by Pazit Zadicario (25211)

“…</p><p></p> For each group five times (bars) are shown: the time spent in the spaces between objects (clear bar) and the time at four objects with the longest duration (dark bars; ranked from left to right in decreasing order). * indicates a significant difference between the time spent between the objects (clear bar) and the first-ranked object in each inset (the left dark bar in each inset) as revealed in a Paired t-test comparison in each test group. …”

Lowering the case numbers without the most stringent restrictions opens a middle ground between freedom and fatalities and prevents a new wave in the long term. by Simon Bauer (11378195)

“…Keeping the gross reproduction number at a moderate level (1.5) until the everyone above 60 has been offered vaccination allows to decrease case numbers steadily. …”

Trends in cutting performance with different chip shapes. by Zhaolong Zhu (5746550)

Attribute values at a single moment. by Nada Ahmed Ezz-Eldien (17396553)

“…The presented algorithm is simulated in a heterogeneous wireless network where the fifth-generation (5G) wireless technology coexists with other radio access networks to improve the evaluation field of the proposed algorithm. …”