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    The point mutants E559A, E563A and K567A display altered DNA-binding kinetics. by Bettina Hüntelmann (566945)

    Published 2014
    “…(G, H) Reduced nuclear export kinetics of STAT1-E559A and -E563A. HeLa cells expressing GFP-tagged wild-type or mutant STAT1 (scale bar 10 µm) were prestimulated for 45 min with IFNγ to induce nuclear accumulation (top panel) and then treated for 6 min in the presence of 50 µg/ml digitonin in ice-cold transport buffer (bottom panel). …”