Image_1_Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.pdf by Taylor L. T. Wherry (10022300)

“…Briefly, PBMCs were isolated and cultured in vitro with 4 ng/ml 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃] or 100 ng/ml 25(OH)D₃. …”

Data_Sheet_1_Effects of 1,25-Dihydroxyvitamin D3 and 25-Hydroxyvitamin D3 on PBMCs From Dairy Cattle Naturally Infected With Mycobacterium avium subsp. paratuberculosis.xlsx by Taylor L. T. Wherry (10022300)

“…Briefly, PBMCs were isolated and cultured in vitro with 4 ng/ml 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃] or 100 ng/ml 25(OH)D₃. …”

Cx26 immunostaining and quantitative analysis in the neuromuscular compartment of normal and diverticular disease human colon. by Letizia Mattii (302997)

“…<p>In normal colon, Cx26 is widely expressed in both circular (CM) and longitudinal muscle (LM) (A), while it is decreased in samples from DD colon (B); scale bar: 100 µm. …”

Although Hrq1 is needed for cisplatin resistance, it is degraded by the proteasome upon cisplatin exposure. by Thong T. Luong (13833935)

“…(C) Hrq1 protein levels are decreased upon cisplatin treatment. Exponentially growing cells with Hrq1-9xMYC were incubated with cycloheximide in the presence or absence of 100 μg/ml cisplatin and/or 0.1% DMSO. …”

miR-155 directly targets the 3′UTR of TRAF3 and STAT1. by Xinyue Zhang (271120)

“…<b>(B and C)</b> miR-155 targets the 3′UTR of TRAF3 and STAT1. 293T cells were co-transfected with a luciferase reporter vector containing the WT or mutant 3′UTR of TRAF3 or STAT1 (100 ng) and 30 nM miR-155 mimics or NC for 48 h, and then harvested for luciferase assay. …”

DataSheet3_Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model.PDF by Noor Azzizah Omar (16880058)

“…Subsequently, adult zebrafish were injected intraperitoneally with 100 μg/g of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and treated with 400 ng/g body weight of recombinant NT3 (rNT3) via intracranial injection 24 h following MPTP injection. …”

DataSheet2_Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model.PDF by Noor Azzizah Omar (16880058)

“…Subsequently, adult zebrafish were injected intraperitoneally with 100 μg/g of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and treated with 400 ng/g body weight of recombinant NT3 (rNT3) via intracranial injection 24 h following MPTP injection. …”

DataSheet1_Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model.PDF by Noor Azzizah Omar (16880058)

“…Subsequently, adult zebrafish were injected intraperitoneally with 100 μg/g of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and treated with 400 ng/g body weight of recombinant NT3 (rNT3) via intracranial injection 24 h following MPTP injection. …”

Transient and stable knock-down of ADAM10 suppresses APLP2 shedding. by Sebastian Hogl (349573)

“…All three species of sAPLP2 (* CS-GAG modified, ** 115 kDa, *** 100 kDa) were strongly decreased upon knock-down of ADAM10, but not of ADAM17. …”

Functional changes of SRA01/04 cells infected with Ad-NDRG2. by Zi-Feng Zhang (341807)

“…<p>A. Shows by Western blot that protein expression of Ndrg2 in SRA01/04 cells is induced when cells are infected with Ad-NDRG2 but not Ad-LacZ (Mean± SD; n = 3. * <i>P</i><0.05 compare with normal SRA01/04 cells). …”

WV phase space projection for QIF model with spike waveform, and convergent iterative theoretical predictions for <i>μ</i> > <i>μ</i>* and <i>μ</i> → 0. by Adam D. Schneider (148548)

“…Inset shows the cyan trajectory in terms of decaying variables <i>x</i>*(<i>t</i>) and <i>C</i>*(<i>t</i>), and how <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159300#pone.0159300.e084" target="_blank">Eq (22)</a> captures the initial increase and then decrease in the calcium concentration, while <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159300#pone.0159300.e083" target="_blank">Eq (21)</a> does not. …”

Data_Sheet_1_The effect of progressive hypoxia on swimming mode and oxygen consumption in the pile perch, Phanerodon vacca.docx by LeeAnn C. Frank (18097492)

“…At this point, we expected a gait transition to occur (i.e., from MPF to BCF propulsion), which is observed by a decrease in pectoral fin beat frequency and an increase in caudal fin or bursting frequency.…”

Moderate number of distance constraints and varying number of sequences required for correct 3D structure prediction. by Debora S. Marks (193565)

“…The point at which a predicted protein structure from a particular family reaches below 4 Å Cα-RMSD varies considerably. …”

Computational analysis distinguishes BMP-FGF cross inhibition models. by Shyam Srinivasan (522850)

“…Based on initial conditions, CIPF produces different B_T response curves, thus displaying hysteresis, while STI, SUI, and CFF produce identical curves. (<b>E</b>) Summary of modeling results. Grid coloring indicates the presence (black), absence (white), or a less than 10% presence of the property (grey) indicated on the left: e.g. …”

imulations showing long-term tumor xenograft response to combination therapy. by Harsh Vardhan Jain (506152)

“…<p>Following the experimental protocol in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081582#pone.0081582-Witham1" target="_blank">[13]</a>, the administration of a weekly dose of 30 mg/kg carboplatin together with a daily dose of 100 mg/kg ABT-737 is simulated, and simulations allowed to run until tumor cell numbers averaged over 7 days (the period of carboplatin administration) achieve a steady-state. …”

MPP2 expression levels influence mIPSC amplitudes. by Bettina Schmerl (669003)

“…<b>(d)</b> Example traces of mIPSC from neurons transduced with control shRNA (black) and MPP2 knockdown shRNA (grey). Scale bar: 100 pA/5s. <b>(e)</b> Knockdown of MPP2 leads to a decrease in average mIPSC amplitude (Mann–Whitney test <i>p</i> = 0.0277). …”

Effect of (CUG)₇ AON on HTT mRNA levels in HD patient derived cell lines 48 hours after transfection. by Melvin M. Evers (207105)

“…Transfection with (CUG)₇ shows a decrease of the upper band, representing the transcript from the mutant allele. …”

Cx43 immunostaining and quantitative analysis in the neuromuscular compartment of normal and diverticular disease human colon. by Letizia Mattii (302997)

“…<p>In normal colon, appreciable amounts of Cx43 are expressed in the circular (CM) and longitudinal muscle (LM) (A), while Cx43 expression is decreased in samples from DD colon (B); scale bar: 100 µm. …”

Lung phenotype of <i>UPK II-Cre;LSL-K-ras^G12D</i> mice. by Francisco Ayala de la Peña (554607)

“…<b>I–J</b>: Morphometric analysis of lung sections showed a decreased total air space (<b>I</b>) and mean alveolar (saccular) area (<b>J</b>) in <i>UPK II-Cre;LSL-K-ras^G12D</i> mice at E17.5 (n = 5); the impairment of air space development led to an increased total air space area and mean alvelolar area in the early postnatal period (P1; n = 12) in transgenic mice when compared to controls; bars, SEM. …”

Sequence motifs function “out of context”. by Ido Yofe (589603)

“…C) Reporter YFP expression is decreased upon exchange of an enhancer to a silencer motif in two independent intron strains. …”