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    Comparing Methods for Induction of Insulin Resistance in Mouse 3T3-L1 Cells.

    Comparing Methods for Induction of Insulin Resistance in Mouse 3T3-L1 Cells. by Al-Jaber, Hend

    Published 2024
    “…These factors included 2.5nM tumor necrosis factor-alpha (TNFα), 20 ng/ml interleukin-6 (IL-6), 10 micromole 4-hydroxynonenal (4HNE), and high insulin (HI) at a concentration of 100nM. To assess cell proliferation, DAPI staining was employed, and the expression of genes associated with various metabolic pathways affected by insulin resistance was investigated using Real-Time PCR. …”
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