SETX (senataxin), the helicase mutated in AOA2 and ALS4, functions in autophagy regulation by Patricia Richard (1341333)

“…</p> <p><b>Abbreviations:</b> 3ʹREADS: 3ʹ region extraction and deep sequencing; ACTB: actin beta; ALS4: amyotrophic lateral sclerosis type 4; AOA2: ataxia with oculomotor apraxia type 2; APA: alternative polyadenylation; AS: alternative splicing; ATG7: autophagy-related 7; ATP6V0D2: ATPase H+ transporting V0 subunit D2; BAF: bafilomycin A₁; BECN1: beclin 1; ChIP: chromatin IP; Chloro: chloroquine; CPT: camptothecin; DDR: DNA damage response; DNMT1: DNA methyltransferase 1; DRIP: DNA/RNA IP; DSBs: double strand breaks; EBs: embryoid bodies; FTD: frontotemporal dementia; GABARAP: GABA type A receptor-associated protein; GO: gene ontology; HR: homologous recombination; HTT: huntingtin; IF: immunofluorescence; IP: immunoprecipitation; iPSCs: induced pluripotent stem cells; KD: knockdown; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MN: motor neuron; MTORC1: mechanistic target of rapamycin kinase complex 1; PASS: PolyA Site Supporting; PFA: paraformaldehyde; RNAPII: RNA polymerase II; SCA: spinocerebellar ataxia; SETX: senataxin; SMA: spinal muscular atrophy; SMN1: survival of motor neuron 1, telomeric; SQSTM1/p62: sequestosome 1; TFEB: transcription factor EB; TSS: transcription start site; TTS: transcription termination site; ULK1: unc-51 like autophagy activating kinase 1; WB: western blot; WIPI2: WD repeat domain, phosphoinositide interacting 2; XRN2: 5ʹ-3ʹ exoribonuclease 2.…”

DataSheet4_Greater than recommended stiffness and power setting of a stance-phase powered leg prosthesis can improve step-to-step transition work and effective foot length ratio du... by Joshua R. Tacca (18067213)

“…Furthermore, we found that use of the BiOM with 10% and 20% greater than recommended power increased AL_trail W_pos and decreased UL_lead W_neg magnitude at 0.75–1.00 m/s. …”

DataSheet3_Greater than recommended stiffness and power setting of a stance-phase powered leg prosthesis can improve step-to-step transition work and effective foot length ratio du... by Joshua R. Tacca (18067213)

“…Furthermore, we found that use of the BiOM with 10% and 20% greater than recommended power increased AL_trail W_pos and decreased UL_lead W_neg magnitude at 0.75–1.00 m/s. …”

DataSheet2_Greater than recommended stiffness and power setting of a stance-phase powered leg prosthesis can improve step-to-step transition work and effective foot length ratio du... by Joshua R. Tacca (18067213)

“…Furthermore, we found that use of the BiOM with 10% and 20% greater than recommended power increased AL_trail W_pos and decreased UL_lead W_neg magnitude at 0.75–1.00 m/s. …”

Measurement of binary and graded signaling responses by flow cytometry. by Ermelinda Porpiglia (34810)

“…Therefore, a plot of total p-Stat5 MFI versus Epo concentration shows a graded response (here, the Hill coefficient is n_H = 1). …”

Photobiomodulation and physical exercise on strength, balance and functionality of elderly women by Patricia Gabrielli Vassão (5223599)

“…The strength training program associated with PBM active improved a significant increase, relative to baseline, to the right medial/lateral stability index (p = 0.007) and decrease in the Fall Risk Test (p = 0.005). …”

Table_1_Learning from local knowledge on changes in tree-cover and water availability: the case of the contested agroforested landscape of the Mt. Elgon Water Tower, Uganda.docx by George Kimbowa (18319500)

“…Farmers and key experts perceived a decrease in water availability and a decrease in overall tree cover for the period 1990–2020. …”

Video_2_A New Antagonist of Caenorhabditis elegans Glutamate-Activated Chloride Channels With Anthelmintic Activity.AVI by María Julia Castro (9254678)

“…To unravel the molecular mechanism, we measured whole-cell currents from GluClα1/β receptors expressed in mammalian cells. …”

Image_2_Genetic Engineering of the Kidney to Permanently Silence MHC Transcripts During ex vivo Organ Perfusion.TIF by Yuliia Yuzefovych (8465751)

“…Nevertheless, cytokine secretion signatures were altered during perfusion with lentiviral vectors as revealed by an increase in the secretion of IL-10, MIP-1α, MIP-2, IP-10, and EGF and a decrease in the levels of IL-12, IL-17, MCP-1, and IFN-γ. …”

Ibuprofen and diclofenac affect STAT-3 signalling differentially. by Verena Leidgens (815986)

“…The STAT-3 downstream target c-myc was significantly upregulated by ibuprofen (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140613#pone.0140613.s005" target="_blank">S5A Fig</a>), whereas diclofenac blocked pSTAT-3 and c-myc in a concentration-dependent manner (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140613#pone.0140613.s005" target="_blank">S5B Fig</a>). …”

Freshwater vertebrate and invertebrate diversity patterns in an Andean-Amazon basin: implications for conservation efforts by Janeth Lessmann (670250)

“…Also, we found a high correlation between species and family richness of freshwater invertebrates (<i>r</i> = 0.66), suggesting that family richness of invertebrates could be used as a surrogate of species richness in this basin. …”

Homogeneous Electrocatalytic Reduction of CO₂ by a CrN₃O Complex: Electronic Coupling with a Redox-Active Terpyridine Fragment Favors Selectivity for CO by Amelia G. Reid (13983239)

“…Here, we report a new CrN₃O catalyst, Cr(tpy^tbupho)Cl₂ (<b>1</b>), where 2-(2,2′:6′,2″-terpyridin-6-yl)-4,6-di-<i>tert</i>-butylphenolate = [tpy^tbupho]⁻, which reduces CO₂ to CO with almost quantitative selectivity via a different mechanism than our previously reported Cr(^tbudhbpy)Cl(H₂O) catalyst. …”

Daily Liquorice Consumption for Two Weeks Increases Augmentation Index and Central Systolic and Diastolic Blood Pressure by Miia H. Leskinen (621293)

“…</p><p>Results</p><p>Two weeks of liquorice ingestion elevated peripheral and central systolic and diastolic blood pressure (by 7/4 and 8/4 mmHg, 95% confidence intervals [CI] 2-11/1-8 and 3-13/1-8, respectively, <i>P</i><0.05), and increased extracellular volume by 0.5 litres (<i>P</i><0.05 versus controls). …”

Analysis of 13,312 benthic invertebrate samples from German streams reveals minor deviations in ecological status class between abundance and presence/absence data by Dominik Buchner (8173911)

“…Broken down to 30 official stream types, we compared assessment results based on abundance and presence/absence data for the assessment modules “organic pollution” (i.e., the saprobic index) and “general degradation” (a multimetric index) as well as their underlying metrics.…”

Hypoxia/Reoxygenation Cardiac Injury and Regeneration in Zebrafish Adult Heart by Valeria Parente (103505)

“…Cardiac function was assessed by 2D-echocardiography and Ventricular Diastolic and Systolic Areas were used to compute Fractional Area Change (FAC). FAC decreased from 29.3±2.0% in normoxia to 16.4±1.8% at 18 h after H/R; one month later ventricular function was back to baseline (n = 12 at each time point).…”

Targeting Membrane-Bound Viral RNA Synthesis Reveals Potent Inhibition of Diverse Coronaviruses Including the Middle East Respiratory Syndrome Virus by Anna Lundin (569801)

“…Besides K22 resistance, the nsp6 mutants induced a reduced number of DMVs, displayed decreased specific infectivity, while RNA synthesis was not affected. …”

Lineage-Through-Time (LTT) plot of Hyalospheniidae. by Omar Fiz-Palacios (457505)

“…Diversification rate shifts leading to a rate increase (7 Myr ago) and a rate decrease (2.5 Myr ago) as detected in LASER are indicated with dotted grey lines. …”

Multiple modes of modulation of PC simple spikes during light activation of ChR2 specifically expressed in GCs. Light activation of GCs results in inhibition and/or activation of P... by Wolfgang Kruse (619565)

“…<p>(A) Increase in PC spike rate during 5 sec light activation of GCs expressing ChR2. …”

Image_4_Co-Exposure of Cardiomyocytes to IFN-γ and TNF-α Induces Mitochondrial Dysfunction and Nitro-Oxidative Stress: Implications for the Pathogenesis of Chronic Chagas Disease C... by João Paulo Silva Nunes (5918402)

“…We found that the STAT1/NF-κB/NOS2 axis is involved in the IFN-γ/TNF-α-induced decrease of ΔΨm in AC-16 cardiomyocytes. …”

Table_3_Co-Exposure of Cardiomyocytes to IFN-γ and TNF-α Induces Mitochondrial Dysfunction and Nitro-Oxidative Stress: Implications for the Pathogenesis of Chronic Chagas Disease C... by João Paulo Silva Nunes (5918402)

“…We found that the STAT1/NF-κB/NOS2 axis is involved in the IFN-γ/TNF-α-induced decrease of ΔΨm in AC-16 cardiomyocytes. …”