Cre-mediated heterozygous deletion of Med28 causes differentiation of iPSC. by Lin Li (28817)

“…<b>(F)</b> Quantitation of real—time PCR for differentiation markers reveal increased differentiation markers in deleted (+Cre) <i>Med28</i>^<i>fl/fl</i> iPSC colonies for extra embryonic lineage (<i>Gata4</i>, <i>Hand1</i>, and <i>Cdx2</i>) and decreased germ layer lineage makers nestin (Nes, ectoderm) and T brachyury (T, mesoderm) compared to non-deleted (-Cre) <i>Med28</i>^<i>fl/fl</i> iPSCs colonies (n = 4). …”

Arrhythmias in Andersen-Tawil syndrome, modeled as dominant negative suppression of IK1 current, have different mechanisms in VM (panel A) versus PF (panel B) myocytes. by Vivek Iyer (572160)

“…<p>For VM, compared to WT (black trace) reduction of IK1 conductance by 90% (blue) leads to a large delayed afterdepolarization. …”

E4orf4 sensitizes cells to killing by drugs inducing DNA damage or replication stress. by Anna Brestovitsky (849835)

“…The number of colonies was counted two weeks later, and relative survival was calculated separately for–E4orf4 and +E4orf4 samples by defining both control samples as 100%. E4orf4 itself decreased cell viability to 40% of control cells. …”

ASC-dependent inflammasomes promote leukocyte migration into the CNS during HSV-1 encephalitis. by Cooper K. Hayes (10063308)

“…<p>Infiltrating leukocytes in the brain were analyzed by flow cytometry in WT, NLRP3^-/-, and ASC^-/- mice at day 3 post-infection after intracranial inoculation with 3 x 10⁶ PFU HSV-1 KOS. …”

An idealized view of the opportunity provided by a comparative and integrated oncology drug development path. by Ira Gordon (369869)

“…<p>This is a theoretical illustration of 100 preclinical agents that may be evaluated by either a conventional or an integrated and comparative drug development path. …”

mRNA sequencing identifies IRE1α- and glucose-dependent mRNAs in islets. by Justin R. Hassler (813970)

“…(B) Four-way Venn diagrams of <i>WT</i>^<i>Fe/+</i> versus <i>KO</i>^{<i>Fe/-; Cre</i>} islets during 6 mM versus 1 8mM glucose exposur<i>e</i> for 72 h. …”

M1 polarization of macrophages upon persistent exposure to androgen. by Qin Lang (2902367)

“…(B, C, and D) Upon stimulation of RAW 264.7 cells with T, the mRNA expression levels of IL-12, iNOS and IL-6, and IL-1β increased significantly on day-1, 2, and 3, respectively, whereas (E, F, G) IL-10 and Arg1 expressions decreased significantly on day-3 (treatment with three T doses at the same time point, *<i>P</i><0.05 and **<i>P</i><0.01 by one-way ANOVA; group treated with 0 nM T versus group treated with 10 nM T, ^#<i>P</i><0.05 and ^##<i>P</i><0.01 by Dunnett’s multiple comparison test; group treated with 0 nM T versus group treated with 100 nM T, ^&<i>P</i><0.05 and ^&&<i>P</i><0.01 by Dunnett’s multiple comparison test).…”

Relative membrane levels of endogenous endoglin in HMEC-1 cells upon transient or stable mutant protein expression. by Tassilo Förg (606727)

“…Cells expressing the variant endoglin^ΔC (DC, without cytoplasmic domain) showed a 1.5 times higher ectopic expression than endoglin^wt.…”

PIAS1 inhibits the ability of TGFβto suppress E-cadherin promoter activity. by Stuart J. Netherton (152258)

“…Lysates of NMuMG cells transfected with the E-cadherin promoter driven luciferase (E-cadherin-p-luciferase) reporter and the β-galactosidase (β-gal) expression construct together with an empty expression vector (−), or one encoding wild type PIAS1 (WT) or SUMO E3 ligase mutant PIAS1 (CS), and left untreated or incubated with 100 pM TGFβ for 48 h, were subjected to luciferase and β-galactosidase assays (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013971#s4" target="_blank">MATERIALS and METHODS</a>). …”

A Randomized, Crossover Study to Evaluate the Pharmacokinetics of Amantadine and Oseltamivir Administered Alone and in Combination by Dennis Morrison (272746)

“…Among the therapies considered for use in pandemic influenza is the co-administration of ion channel and neuraminidase inhibitors, both to potentially increase efficacy as well as to decrease the emergence of resistant isolates. To better understand the potential for drug interactions, a cross-over, randomized, open-label trial was conducted with amantadine, 100 mg po bid, and oseltamivir, 75 mg po bid, given alone or in combination for 5 days. …”

Effect of <i>OVOS2</i> downregulation on the cell invasive behavior of A375. by Ying-Xue Huang (5130974)

“…Representative results from three independent experiments were shown (100×). (<b>c</b>) The inhibition of the transmigration ability during 24 h culture of A375 cells transfected with <i>OVOS2</i>-shRNA was shown using the Transwell migration study (*<i>P</i> < 0.05, <i>P</i> = 0.016 and <i>P</i> = 0.02, compared with A375/NC-shRNA and untreated A375 cell groups, respectively; ANOVA, LSD multiple comparison); and a nonsignificant decrease of invasion ability during 48 h culture of A375 cells transfected with <i>OVOS2</i>-shRNA was shown by the Matrigel invasion assay (<i>P</i> = 0.453, ANOVA). …”

METTL3 and ALKBH5 oppositely regulate m⁶A modification of <i>TFEB</i> mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes by Huiwen Song (755791)

“…</p> <p>ACTB, actin beta; ALKBH5, alkB homolog 5, RNA demethylase; ANXA5, annexin A5; ATG, autophagy-related; BafA, bafilomycin A₁; CASP3, caspase 3; ELAVL1, ELAV like RNA binding protein 1; FTO, FTO, alpha-ketoglutarate dependent dioxygenase; GFP, green fluorescent protein; GST, glutathione S-transferase; HNRNPD, heterogeneous nuclear ribonucleoprotein D; H/R, hypoxia/reoxygenation; I/R, ischemia/reperfusion; LAD, left anterior descending; m⁶A, N⁶-methyladenosine; MEFs, mouse embryo fibroblasts; Mer, mutated estrogen receptor domains; METTL3, methyltransferase like 3; METTL14, methyltransferase like 14; mRFP, monomeric red fluorescent protein; MTORC1, mechanistic target of rapamycin kinase complex 1; NMVCs, neonatal mouse ventricular cardiomyocytes; PCNA, proliferating cell nuclear antigen; PE, phosphatidylethanolamine; PI, propidium iodide; PTMs, post-translational modifications; PVDF, polyvinylidenedifluoride; RIP, RNA-immunoprecipitation; siRNA, small interfering RNA; SQSTM1, sequestosome 1; TFEB, transcription factor EB; TUBA: tublin alpha; WTAP, WT1 associated protein; YTHDF, YTH N6-methyladenosine RNA binding protein</p>…”

A scoring strategy for progression risk and rates of treatment completion in subjects with latent tuberculosis by Michael Scolarici (5979566)

“…In this study our objective was 1) To retrospectively apply the online calculator (<a href="http://tstin3d.com" target="_blank">tstin3d.com</a>) to determine the probability of having LTBI and assign cumulative risk of progression. 2) Measure treatment outcomes in subjects with Low: 0-<10%, Intermediate: 10-<50% and High: 50–100% cumulative risk. …”

Capsaicin inhibited the proliferation of human SCLC cells in a time- and concentration-dependent manner. by Kathleen C. Brown (365508)

“…<p>(A) MTT assays show at the treatment of H69 and H82 cells with 50 µM capsaicin causes a decrease in cell viability starting from 24 hours to 72 hours. …”

A case for revisiting peer review: Implications for professional self-regulation and quality improvement by Terry E. Hill (5465027)

“…</p><p>Results</p><p>Change point analysis reveals with 98% confidence that a significant improvement in age-adjusted natural mortality occurred in 2007, decreasing from 138.7 per 100,000 in the 1998–2006 period to 106.4 in the 2007–2009 period. …”

Image_4_Astrocytic Ephrin-B1 Controls Synapse Formation in the Hippocampus During Learning and Memory.TIF by Amanda Q. Nguyen (8584413)

“…In contrast, ephrin-B1 OE in hippocampal astrocytes impaired dendritic spine formation and clustering, specifically on c-Fos(+) neurons, coinciding with an overall decrease in vGlut1/PSD95 co-localization. …”

Image_3_Astrocytic Ephrin-B1 Controls Synapse Formation in the Hippocampus During Learning and Memory.TIF by Amanda Q. Nguyen (8584413)

“…In contrast, ephrin-B1 OE in hippocampal astrocytes impaired dendritic spine formation and clustering, specifically on c-Fos(+) neurons, coinciding with an overall decrease in vGlut1/PSD95 co-localization. …”

Image_2_Astrocytic Ephrin-B1 Controls Synapse Formation in the Hippocampus During Learning and Memory.TIF by Amanda Q. Nguyen (8584413)

“…In contrast, ephrin-B1 OE in hippocampal astrocytes impaired dendritic spine formation and clustering, specifically on c-Fos(+) neurons, coinciding with an overall decrease in vGlut1/PSD95 co-localization. …”

Data_Sheet_1_A Linear Fragment of Unacylated Ghrelin (UAG6−13) Protects Against Myocardial Ischemia/Reperfusion Injury in Mice in a Growth Hormone Secretagogue Receptor-Independent... by David N. Huynh (6198476)

“…The infarct area was decreased in a dose-dependent manner at 48 h of reperfusion, with a reduction of 54% at the highest dose of UAG₆₋₁₃ tested. …”

Effects of BMAL activator level on circadian oscillations in a stochastic model of isolated cellular oscillators. by Caroline H. Ko (240081)

“…All (243 of 243) <i>Bmal1</i>^−/− cells were equal to or below a 0.1 cutoff value for circadian rhythmicity (indicated by the dotted line), whereas approximately 80% of WT neurons were above this cutoff value and displayed circadian rhythmicity. …”