Allele-specific silencing of mutant human ataxin-3 mediates robust reduction of the number of ataxin-3 inclusions and preservation of DARPP-32 immunoreactivity in the rat striatum. by Sandro Alves (376415)

“…The vectors encoding the shRNA cassette and the <i>lacZ</i> reporter gene infect an extensive region of the rat striatum, as shown by β-galactosidase immunoreactivity (A, B and C). shAtaxMUT specifically downregulates MUT ATX3, promoting a significant decrease in the number of MUT ATX3-positive aggregates (E and H ), whereas shAtaxWT has almost no effect on MUT ATX3 expression (F and I), as shown by comparison with the results obtained with the mistargeted shGFP (D and G). …”

Enhanced home cage activity in RAGE KO mice. by Seiichi Sakatani (255810)

“…The activity of KO mice in dark phase is higher than that of WT mice. Note that both WT and KO showed a gradual decrease in activity in the dark during the course of the seven days. …”

Rho A activity is reduced in FLNa null cells by Chunxiang Sun (477083)

“…<p>WT and FLNa null neutrophils in suspension (<b>A</b>) or plated on dishes (<b>B</b>) for 20 minutes are stimulated with 1μM fMLP for 30, 60, and 300 seconds. …”

Effects of RNA on S/MAR-selective binding of hnRNP U. by Mary Miyaji (16764659)

“…Bars plotted are average of 3 experiments with standard deviation. The decrease in aggregated DNA at 1x fold RNA was statistically significant by Student’s <i>t</i> test (marked by asterisk, <i>p</i><0.01). …”

Immunofluorescence assay analyses of RPS13 and RPL22. by Samuel L. Hutson (236870)

“…<p>(<b>A–C</b>) Immunofluorescence assay analysis of the expression of RPS13 in wildtype (wt,A) and <i>Δrps13</i> (B) parasites at 4 and 24 hours of culture ±ATc. …”

Functional characterization of differentiated astrocytes. by In Ki Cho (6494564)

“…(D) Significant decreases in the amplitude of peak ramp currents were observed in WT astrocytes following 20 min application of TBOA at the concentration of 500 uM (n = 6). …”

D-Serine and Glycine Differentially Control Neurotransmission during Visual Cortex Critical Period - Fig 2 by Claire N. J. Meunier (2579599)

“…Scale bars: 100pA, 500ms. <b>B:</b> Further, enhancing endogenous glycine levels with the glycine transporter blocker ALX5407 (2 μM) decreased the NMDARs response, an effect blocked by <i>Bs</i>GO (n = 4 cells, 4 slices, 2 animals). …”

Lack of LeuB results in increased proteolytic activity during iron starvation caused by leucine shortage. by Nanbiao Long (5900231)

“…(I) Western blot analysis of actin demonstrating that the proteolytic activity caused by lack of LeuA with 1 mM leucine supplementation is not rescued by iron supplementation (50 μM).…”

RFB-dependent CAG-70 repeat expansions occur through a Rad52- and Rad8^{ScRad5/HsHTLF}-mediated mechanism. by Michaela A. Gold (11592777)

“…C) Fold over WT percent expansions for CAG-70 repeat in the following mutants: <i>rad52Δ</i> at 1.9 kb, <i>rad52Δ</i> at 6.7 kb, and <i>rad8Δ</i> at 1.9 kb, across conditions as indicated. …”

SIRT5 is a proviral factor. by Marius Walter (13775591)

“…Data show fold-changes compared to WT levels at MOI = 0.1. n = 4. <b>C</b>. Decrease in viral titers in <i>SIRT5-</i> and <i>SIRT1-</i>KO cells infected with SARS-Cov-2 for 3 days at MOI = 1, as shown by plaque assay. n = 4. …”

Electrophysiological and pharmacological properties of I_HCN/I_f on single-channel level from inside-out recordings. by Guido Michels (378142)

“…<p>(A) Representative HCN2 single-channel currents (sampling frequency: 5 kHz, corner frequency: 1 kHz) of inside-out recordings from one- and multi-channel (n∼50 channels) patches at different test-potentials (−90 to −130 mV). …”

Microtubular PEDOT-Coated Bricks for Atmospheric Water Harvesting by Hongmin Wang (638670)

“…Our brick composite achieves a maximum water vapor uptake of ∼200 wt % versus polymer mass at 95% relative humidity, decreasing to ∼15 wt % at 40% relative humidity. …”

Table_3_Identification and Clinical Validation of Key Extracellular Proteins as the Potential Biomarkers in Relapsing-Remitting Multiple Sclerosis.xlsx by Meng Li (79487)

Table_4_Identification and Clinical Validation of Key Extracellular Proteins as the Potential Biomarkers in Relapsing-Remitting Multiple Sclerosis.xlsx by Meng Li (79487)

Table_5_Identification and Clinical Validation of Key Extracellular Proteins as the Potential Biomarkers in Relapsing-Remitting Multiple Sclerosis.xlsx by Meng Li (79487)

Table_2_Identification and Clinical Validation of Key Extracellular Proteins as the Potential Biomarkers in Relapsing-Remitting Multiple Sclerosis.xlsx by Meng Li (79487)

Mutators are under strong pressure to revert in mosquito models. by Kathryn Rozen-Gagnon (510005)

“…<p>(A–C) <i>Ae. aegypti</i> or (D–F) <i>Ae. albopictus</i> were infected with WT and mutator strains of CHIKV, and mosquitoes were collected at 1, 3, 7 and 14 days post-infection. …”

The Influence of the Platinum Weight Percentage in the Catalyst Formulation on the Performance of a High Temperature Ethanol Electroreformer Based on H3PO4 Doped Polybenzimidazole... by Bruno G. C. Alves (14005656)

“…In terms of the ethanol electro-oxidation activity, the Pt wt.% impacts on the performance of the electroreformer, with the H2 flux that increase from 0.22 STP (standard pressure and temperature, 273.15 K and 105 Pa) m3 m-2 h-1 for Pt black to 1.47 STP m3 m-2 h-1 for 60 wt.% Pt/C. …”

Targeted expression of TeTxLC, Shi, Kirand Glued-DN affects the architecture of the CSDn by Bidisha Roy (37349)

“…The neuron expressing inactive tetanus toxin (UAS-/+; RN2--FRT-CD2-FRT-Gal4, UAS-/+) have dendrites (arrow in (b)) and terminal arbors (h) comparable to WT. Ectopic expression of active form of tetanus toxin (UAS-/+; RN2--FRT-CD2-FRT-Gal4, UAS-/+) results in dramatic increase in ipsilateral dendrites (arrows in (c)) and a marked decrease in contralateral terminals (i). …”

Reduced EPDC invasion correlates with deficient coronary vascular plexus development. by Andrew B. Harvey (21587431)

“…<b>(E, F)</b> Immunofluorescent stains marking vasculature (PECAM1, green), myocardium (MF20, blue), and SOX18 (red) suggest a decrease in SOX18 expression in the vasculature of <i>Wt1</i>^Cre; <i>Sox9</i>^fl/fl; <i>R26</i>^mG specimens compared to controls at E15.5. …”