Long non-coding RNA MALAT1 promotes steatosis in HepG2 cells by modulating target gene expression in presence of Exendin-4 by Olfa Khalifa (10914452)

“…Following the starvation, a 16h incubation in DMEM containing increasing concentrations of OA (0–500 µM) at 37 °C was performed, and steatosis was quantified by triglycerides quantification, Bodipy staining, and quantification of the expression of perilipin genes family. …”

Antibody stability: A key to performance - Analysis, influences and improvement by Hui Ma (290168)

“…Here we review antibody stability under five headings: (i) measurement techniques; (ii) stability issues in expression and production (expression, proteolysis, aggregation); (iii) effects of antibody format and engineering on stability and (iv) formulation, drying and storage conditions. …”

Squamous Cell Carcinomas of the Head and Neck Cancer Response to Programmed Cell Death Protein-1 Targeting and Differential Expression of Immunological Markers: A Case Report by Maysaloun Merhi (4246147)

“…The PD-1 expression by the patient’s T cells was reduced 15-folds after nivolumab treatment and was uniquely restricted to the CD8+ T cells population. …”

Exendin-4 promotes GSIS without increasing β-cell number through upregulation of glucose-sensing apparatus and mitochondrial oxidative phosphorylation machinery genes in hPSC-deriv... by Abdoulaye Diane (14152749)

“…Interestingly, addition of Exendin-4 (50 nM) during the last 3 days of the differentiation significantly enhanced GSIS associated with increased expression of glucose-sensing apparatus genes (Glut2, G6P2C, GCK) and genes encoding mitochondrial oxidative phosphorylation machinery.…”

Cigarette smoke extract induces p38-mediated expression and ROS/rho-mediated translocation of alpha 2C adrenoceptor in human microvascular smooth muscle cells by Manal M. Fardoun (22303813)

“…This increase is associated with the activation of <u>p38 MAPK</u>, as pretreatment with SB-202190, a p38 specific inhibitor, attenuated CSE-induced α₂_C-AR expression. …”

Downregulation of Lymphoid enhancer‐binding factor 1 (<i>LEF‐1)</i> expression (by immunohistochemistry and/ flow cytometry) in chronic Lymphocytic Leukemia with atypical immunophe... by Dina S. Soliman (14779333)

“…Absence of <i>LEF-1</i> expression in CLL/SLL is correlated (<i>P</i> < .05) with downregulation of CD5, CD23, CD200, expression of FMC7, brighter expression of CD79b, brighter expression of surface light chain, increased prolymphocytes and lower Matutes score.…”

Nimesulide, a preferential cyclooxygenase 2 inhibitor, suppresses peroxisome proliferator‐activated receptor induction of cyclooxygenase 2 gene expression in human synovial fibrobl... by Faour, Wissam

“…Receptor activation studies were evaluated by cotransfecting pSG5-Gal 4 DNA binding domain (DBD)-PPARα ligand binding domain (LBD) or pSG5-Gal 4 DBD-PPARγ LBD chimeric constructs with a 5× Gal 4 enhancer site tk-tataa-luciferase reporter under ligand stimulation in the presence or absence of increasing concentrations of NIM. Gene transactivation analyses were conducted by treating cells overexpressing cytomegalovirus (CMV)-PPARα or CMV-PPARγ expression constructs with either a PPAR response element (PPRE)-luciferase construct containing 3 DR1 acyl-coenzyme A (acyl-CoA) oxidase gene response elements or human COX-2 promoter constructs with WY-14,643, ciglitasone, and 15d-PGJ2 in the presence or absence of increasing concentrations of NIM. …”
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20-HETE and EETs in Diabetic Nephropathy: A Novel Mechanistic Pathway by Stephanie Eid (440990)

“…HG treatment increased ROS production and was associated with alteration in CYPs 4A and 2C11 expression concomitant with alteration in 20-HETE and EETs formation. …”

Adsorption of phosphate on iron oxide doped halloysite nanotubes by Dema A. Almasri (14152743)

Differences in protein expression, at the basal state and at 2 h of insulin infusion, in muscle biopsies from healthy Arab men with high or low insulin sensitivity measured by hype... by Ilham Bettahi (363910)

“…Among the 924 proteins detected in both groups, three were suppressed and three were increased significantly in the LIS subjects compared with the HIS subjects. …”

Phospho-MEK1/2 and uPAR Expression Determine Sensitivity of AML Blasts to a Urokinase-Activated Anthrax Lethal Toxin (PrAgU2/LF) 1 by Nasser, Selim

“…Cytotoxicity of primary AML blasts was also dependent on uPAR expression and phos-MEK1/2 levels. CD34+ bone marrow blasts and peripheral blood mononuclear cells lacked uPAR expression and were resistant to PrAgU2/LF, demonstrating the lack of toxicity to normal hematological cells and, therefore, the tumor selectivity of this approach. …”
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An experimental and first principles DFT investigation on the effect of Cu addition to Ni/Al₂O₃ catalyst for the dry reforming of methane by Anjaneyulu Chatla (4888933)

“…DFT calculations reveal increased energy barrier for carbon adsorption, whilst promoting facile removal of deposited carbon. …”

Toxicity assessment of di(2-ethylhexyl) phthalate using zebrafish embryos: Cardiotoxic potential by Azza Naïja (19137067)

“…Although DEHP showed negligible influence on the length and pericardial area of exposed embryos, it led to multiple bodily deformities. Gene expression analyses of key cardiogenic and inflammatory genes evidenced alterations in tbx20, bcl2, and il1b expression in Zebrafish embryos at 96 h post-fertilization. …”

A data browsing application for accessing gene and module-level blood transcriptome profiles of healthy pregnant women from high- and low-resource settings by Darawan Rinchai (742366)

“…Additional blood transcriptome profiles were retrieved from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) public repository, representing a cohort of healthy pregnant women from a high-resource setting. …”

DNAJB3/HSP-40 cochaperone improves insulin signaling and enhances glucose uptake in vitro through JNK repression by Mohamed Abu-Farha (25322)

“…Nevertheless, DNAJB3 expression pattern in diabetic subjects and its mode of action are not yet known. …”

A collection of annotated and harmonized human breast cancer transcriptome datasets, including immunologic classification by Jessica Roelands (7516439)

“…<p dir="ltr">The increased application of high-throughput approaches in translational research has expanded the number of publicly available data repositories. …”

Prostaglandin E2 stimulates p53 transactivational activity through specific serine 15 phosphorylation in human synovial fibroblasts ROLE IN SUPPRESSION OF c/EBP/NF-κB-MEDIATED MEKK... by Faour, Wissam

“…PGE2 induced a time-dependent increase in p53 Ser15 phosphorylation, with no discernible change in overall p53 levels. …”
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PRAME promotes epithelial-to-mesenchymal transition in triple negative breast cancer by Ghaneya Al-Khadairi (14153274)

“…In line with this, interrogation of TCGA and GTEx data demonstrated an increased PRAME expression in ovarian and esophageal tumor tissues in addition to breast tumors where it is associated with worse survival. …”

Multi-omics and machine learning reveal context-specific gene regulatory activities of PML::RARA in acute promyelocytic leukemia by William Villiers (19482292)

“…While known to be recruited to binding sites across the genome, its impact on gene regulation and expression is under-explored. …”

Rapid generation of purified human RPE from pluripotent stem cells using 2D cultures and lipoprotein uptake-based sorting by Fabio Michelet (8396283)

“…<h3>Background</h3><p dir="ltr">Despite increasing demand, current protocols for human pluripotent stem cell (hPSC)-derived retinal pigment epithelium (RPE) remain time, labor, and cost intensive. …”