BA attenuated the decrease in the expression of ZO-1 and Occludin induced by LPS in Caco2 cells. by Luqiong Liu (11537092)

BA attenuated the decrease in the integrity and increase in the permeability of the epithelial barrier injury induced by LPS in Caco2 cell monolayers. by Luqiong Liu (11537092)

Y-27632 collaborated with BA to attenuate the increase in the integrity and decrease in the permeability of epithelial barrier injury induced by LPS in Caco2 monolayers. by Luqiong Liu (11537092)

Y-27632 synergized with BA to alleviate the decrease in the expression of and abnormal localization of ZO-1 and Occludin in LPS-induced Caco2 cells. by Luqiong Liu (11537092)

<i>Leptospira interrogans</i> from Okinawa induces epithelial barrier disruption with different kinetics. by Tetsuya Kakita (4922584)

PGAM5 knockdown prevented rTsSPc from disrupting Caco-2 monolayer integrity. by Qi Qi Lu (17721401)

Raw data of Figs 1–6 in this study. by Qi Qi Lu (17721401)

“…The results of qPCR and Western blot revealed that rTsSPc significantly up-regulated the expression of Caco-2 cell apoptosis-related proteins (Caspase 3, Caspase 9 and Caspase 8, Bax and Cytochrome c), activated the apoptosis pathway, and thereby induced Caco-2 apoptosis. rTsSPc bound and interacted with PGAM5 receptor in Caco-2 cells and induced apoptosis, reduced the tight junctions (TJs) expression levels and damaged the Caco-2 monolayer integrity and barrier function, and promoted larval invasion. …”

S1 File - by Luqiong Liu (11537092)

“…Compared with LPS treatment alone, BA significantly mitigated the reduction in the TEER, decreased FD-4 flux permeability, increased the mRNA expression of ZO-1 and Occludin, and normalized the distribution of ZO-1 and Occludin in Caco2 cells. …”

Experimental treatments and groups. by Luqiong Liu (11537092)

“…Compared with LPS treatment alone, BA significantly mitigated the reduction in the TEER, decreased FD-4 flux permeability, increased the mRNA expression of ZO-1 and Occludin, and normalized the distribution of ZO-1 and Occludin in Caco2 cells. …”

Primers for RT-qPCR. by Luqiong Liu (11537092)

“…Compared with LPS treatment alone, BA significantly mitigated the reduction in the TEER, decreased FD-4 flux permeability, increased the mRNA expression of ZO-1 and Occludin, and normalized the distribution of ZO-1 and Occludin in Caco2 cells. …”

Data Sheet 1_Cynaroside ameliorates TNBS-induced colitis by inhibiting intestinal epithelial cell apoptosis via the PI3K/AKT signalling pathway.pdf by figshare admin frontiersin (4545187)

“…Additionally, the Cyn intervention group showed significant improvements in both the intestinal barrier structure (elevated tight junction protein levels and proper localization) and function (reduced serum FD4 levels, increased intestinal TEER, and decreased bacterial translocation rates in mesenteric lymph nodes [MLNs] and livers). …”