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PKA signaling stimulated by the combination of isoproterenol and IBMX is decreased by the absence of insulin.
Published 2020“…(B) Quantitation of mean fluorescence intensity (MFI) for PKA-substrate are presented as whisker plots that encompass data from at least 18 cells, as detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0231806#pone.0231806.g001" target="_blank">Fig 1</a> (n = 3 biological replicates, and at least 6 cells per experiment). (*) Isoproterenol and IBMX treatment caused a significant increase (<i>p</i> < .001) compared to IBMX alone in all conditions. (‡) stimulated Akita and WT–ins conditions are significantly decreased compared to matching WT +ins conditions. …”
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Phosphorylation of PFK2 is decreased in cardiomyocytes cultured in the absence of insulin and with diabetes.
Published 2020“…(B) Quantitation of mean fluorescence intensity (MFI) for pPFK2 are presented as whisker plots that encompass data from at least 18 cells, as detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0231806#pone.0231806.g001" target="_blank">Fig 1</a> (n = 3 biological replicates, and at least 6 cells per experiment). (*) IBMX or Isoproterenol treatment causes a significant increase (<i>p</i> < .001) compared to untreated WT cells; (†) Each WT (-ins) condition is significantly decreased (<i>p</i> < .001) compared to WT (+ins); (‡) all Akita conditions are significantly decreased (<i>p</i> < .001) compared to comparable WT conditions. …”
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