Image 3_Absolute abundance calculation enhances the significance of microbiome data in antibiotic treatment studies.tif by Stefanie Wagner (743707)

“…In another experimental setting with tulathromycin treatment, bacterial abundance quantification by flow cytometry and by a spike-in method yielded similar results only on the phylum level. Even though the spike-in method identified the decrease of four genera, analysis by fluorescence-activated cell sorting (FACS) uncovered eight significantly reduced genera, such as Prevotella and Paraprevotella upon antibiotic treatment. …”

Image 1_Absolute abundance calculation enhances the significance of microbiome data in antibiotic treatment studies.tif by Stefanie Wagner (743707)

“…In another experimental setting with tulathromycin treatment, bacterial abundance quantification by flow cytometry and by a spike-in method yielded similar results only on the phylum level. Even though the spike-in method identified the decrease of four genera, analysis by fluorescence-activated cell sorting (FACS) uncovered eight significantly reduced genera, such as Prevotella and Paraprevotella upon antibiotic treatment. …”

Image 2_Absolute abundance calculation enhances the significance of microbiome data in antibiotic treatment studies.tif by Stefanie Wagner (743707)

“…In another experimental setting with tulathromycin treatment, bacterial abundance quantification by flow cytometry and by a spike-in method yielded similar results only on the phylum level. Even though the spike-in method identified the decrease of four genera, analysis by fluorescence-activated cell sorting (FACS) uncovered eight significantly reduced genera, such as Prevotella and Paraprevotella upon antibiotic treatment. …”

Image 4_Absolute abundance calculation enhances the significance of microbiome data in antibiotic treatment studies.tif by Stefanie Wagner (743707)

“…In another experimental setting with tulathromycin treatment, bacterial abundance quantification by flow cytometry and by a spike-in method yielded similar results only on the phylum level. Even though the spike-in method identified the decrease of four genera, analysis by fluorescence-activated cell sorting (FACS) uncovered eight significantly reduced genera, such as Prevotella and Paraprevotella upon antibiotic treatment. …”

Primer sequences for RT-qPCR. by Wenlong Shen (9313937)

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siRNA sequences and negative controls sequences. by Wenlong Shen (9313937)

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Antibodies used in the study. by Wenlong Shen (9313937)

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Total organic carbon and phenolic content of raw materials and fruit body cultivated (see S2 in S2 File for reference). by Caglar Akcay (6910697)

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Spawn run time, earliness, yield, biological efficiency and dry matter of mushroom fruit body (see S1 in S2 File for reference). by Caglar Akcay (6910697)

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S2 File - Valorization of various lignocellulosic wastes to <i>Ganoderma lucidum</i> (Curtis) P. Karst (Reishi Mushroom) cultivation and their FT-IR assessments by Caglar Akcay (6910697)

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S1 File - Valorization of various lignocellulosic wastes to <i>Ganoderma lucidum</i> (Curtis) P. Karst (Reishi Mushroom) cultivation and their FT-IR assessments by Caglar Akcay (6910697)

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Elemental analysis of substrates and fruit bodies cultivated. by Caglar Akcay (6910697)

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Substrate codes and ratios used in the study. by Caglar Akcay (6910697)

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FT-IR results. by Caglar Akcay (6910697)

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FT-IR results. by Caglar Akcay (6910697)

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FT-IR results. by Caglar Akcay (6910697)

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FT-IR results. by Caglar Akcay (6910697)

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Chemical analysis of the substrates before and after cultivation (see S3 in S2 File for reference). by Caglar Akcay (6910697)

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FT-IR results. by Caglar Akcay (6910697)

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FT-IR results. by Caglar Akcay (6910697)

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