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    Thioflavin-T (ThT) fluorescence analysis of amyloid fibril formation in wild-type and p.R163C mutant αB-crystallin under thermochemical stress. by Aref Baharvand (21713886)

    Published 2025
    “…Samples (0.15 mg/mL) were mixed with 20 µM ThT and incubated in the dark for 5 minutes, after which fluorescence emission (450–600 nm, excitation at 440 nm) was recorded using a Varian Cary Eclipse spectrofluorometer. The plot shows increased ThT fluorescence in the p.R163C mutant compared to wild-type after stress, indicating a higher propensity for amyloid formation or increased β-sheet content, although the differences are not statistically significant. …”
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