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    Cyclization Decoded: Engineering Amylomaltase for Efficient α‑Glucan Transformations حسب Han Liu (288039)

    منشور في 2025
    "…Furthermore, we identify that postglycosylation noncovalent polysaccharide chain transfer emerges as the decisive factor in cyclization, particularly for AM–degree of polymerization (DP) greater than 30 substrate complexes, where this noncovalent step (≥13.6 kcal/mol) dictates the rate of CA production. 57 variants with enhanced activity (up to a 2.3-fold increase) were engineered in our biochemical experiments by strategically modulating the chain transfer step. …"