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    Optimisation of read depth, DNA quantity, and unique alternate observation threshold. by Melinda L. Tursky (20790436)

    Published 2025
    “…The mean number of variants detected per sample above the standard CHIP threshold of ≥ 0.02 VAF increased linearly with subject age (E). Greater than 99.9% of variants detected were below ≥ 0.02 VAF, potentially including those with prognostic or predictive clinical relevance (F). …”