Regulating Effects of Three Polysaccharides on Gut Microbiota in Felines and Canines: Insights from <i>In Vitro</i> Digestion, Fecal Fermentation, and Lactic Acid Bacterial Ferment... by Huasong Bai (17835877)

“…The FU produced the highest lactate concentration. The YG significantly increased SCFA levels, especially in canines, surpassing fructooligosaccharides. …”

Differential expression profiling in the ASFV-ΔI7L-infected primary porcine alveolar macrophages (PAMs) by RNA sequencing analysis. by Meilin Li (1832905)

Heatmap analysis for technical reproducibility of Cohort #1 CP dataset (28wk-old WT and R14^Δ/+). by Brian Foo (19273132)

Vps34 supports Treg maintenance in lymphoid and non-lymphoid tissues. by Erienne G. Norton (9612079)

“…(<b>E</b>) CD4⁺Foxp3-YFP⁺ Tregs (CD45.2⁺) were sort-purified from the spleen of control (<i>n</i> = 4) or <i>Foxp3</i>^Cre<i>Pik3c3</i>^fl/fl (<i>n</i> = 5) mixed BM chimera mice and profiled by microarray analysis (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003074#sec010" target="_blank">Materials and methods</a> for details). GSEA enrichment plots showing increased cTreg signature (left; i.e., top 200 upregulated genes [log₂FC > 0.5, FDR < 0.05] in cTregs versus eTregs) and decreased eTreg signature (right; i.e., top 200 upregulated genes [log₂FC > 0.5, FDR < 0.05] in eTregs versus cTregs) in CD45.2⁺ Tregs from <i>Foxp3</i>^Cre<i>Pik3c3</i>^fl/fl mixed BM chimera mice compared to control mixed BM chimera mice. …”

Vps34 orchestrates transcriptional and epigenetic remodeling events corresponding to terminal eTreg differentiation during perinatal life. by Erienne G. Norton (9612079)

“…GSEA, gene set enrichment analysis; NES, normalized enrichment score; FDR, false discovery rate. …”

Chromatin accessibility analysis of perinatal Vps34-deficient Tregs. by Erienne G. Norton (9612079)

“…(<b>B</b>) Quantification of relative (normalized to average control in each experiment) gMFIs of CellROX and MitoSOX in TCRβ⁺CD4⁺Foxp3-YFP⁺CD44^lowCD62L^high cTregs and TCRβ⁺CD4⁺Foxp3-YFP⁺CD44^lowCD62L^high eTregs derived from the spleen of 7- to 11-day-old perinatal control or <i>Foxp3</i>^Cre<i>Pik3c3</i>^fl/fl mice, as determined by flow cytometry analysis (<i>n</i> =  15 for control and 14 for <i>Foxp3</i>^Cre<i>Pik3c3</i>^fl/fl for both CellROX and MitoSOX). (<b>C</b>) GSEA enrichment plot showing decreased Hallmark TNFα signaling via NFκB signature in Vps34-deficient versus control terminal eTregs from single-cell transcriptome profiling (as described in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003074#pbio.3003074.g003" target="_blank">Fig 3</a>). …”

Atg14 plays a cell-intrinsic role in orchestrating eTreg survival but not terminal differentiation. by Erienne G. Norton (9612079)

“…Jun/Fos transcription factors with predicted decreased activity in Atg14-deficient versus control eTregs are labeled. …”

PERCOM, an unbiased and easily-accessible protein-centric analysis workflow. by Brian Foo (19273132)

Basal HLH-30 activity is not accompanied by enriched nuclear localization and declines during neuronal aging. by Ruiling Zhong (22339779)

Information of core targets. by Guobing Wang (288885)

“…The difference between the single drug group and the corresponding low dose group was significant (P<0.001). After Hela cells were cultured for 72 h, the mRNA and protein expressions corresponding to EGFR, TNF-α, VEGFA, PI3K, AKT and MDM2 were decreased in the experimental group compared with the control group as confirmed by RT-PCR and Western blot, and the extent of the decrease was more obvious with the increase of the drug concentration, and under the condition of the same drug dosage, the effect of the Baicalein group was more pronounced than that of the Wogonin group, and the effect was more obvious in the two-drug group compared with the single-drug group (P<0.05).…”

Composition of Scutellaria Barbata. by Guobing Wang (288885)

“…The difference between the single drug group and the corresponding low dose group was significant (P<0.001). After Hela cells were cultured for 72 h, the mRNA and protein expressions corresponding to EGFR, TNF-α, VEGFA, PI3K, AKT and MDM2 were decreased in the experimental group compared with the control group as confirmed by RT-PCR and Western blot, and the extent of the decrease was more obvious with the increase of the drug concentration, and under the condition of the same drug dosage, the effect of the Baicalein group was more pronounced than that of the Wogonin group, and the effect was more obvious in the two-drug group compared with the single-drug group (P<0.05).…”

Reduced mitochondrial function and protein expression in 21-28wk-old R14^Δ/+ mice. by Brian Foo (19273132)

Graphical overview of study. by Brian Foo (19273132)

A review of geological characteristics and selenium enrichment in Se-bearing volcanogenic massive sulfide deposits by Wei Zhang (405)

“…<p>Volcanogenic massive sulphide (VMS) deposits consist of upper stratabound massive-to-semimassive sulphide lenses and discordant stringer sulphides in the footwall, hosting significant base metals like Zn, Cu, and Pb, with some enriched in precious (Ag, Au) and semi-metals (like Se and Te). …”

TMS recapitulates epigenetic age predictions and tissue-dependent effects identified via other technologies. by Amy Longtin (21409957)

GO and KEGG analysis of DEPs. by Huiyang Jia (14444661)

Top 50 hub genes in modules 3 (M3) and 4 (M4). by Gaurab Bhattarai (20308531)

<b>Phenological changes mediated the effects of nitrogen enrichment on reproduction in an alpine meadow on the Tibetan Plateau</b> by Tianwu Zhang (22122382)

“…Notably, the interactive effects of N and precipitation addition significantly enhanced fruit set. Phenological shifts mediated plant reproductive responses to N addition and altered precipitation. …”

GO and KEGG pathway analyses of the DE-mRNAs. by Meimei Xu (744867)

“…The color transition from blue to red indicates decreasing <i>P</i> values, indicating increasingly significant differences. …”

S5 Fig - by Abishek Balakumar (20189865)