BAR-CAT: Targeted Recovery of Synthetic Genes via Barcode-Directed CRISPR-dCas9 Enrichment by Natanya Villegas (20912585)

“…After a single 15-min reaction and optimized wash regime (BAR-CAT v1.0), three low-abundance targets in a 300,000-member test library were enriched 600-fold, greatly reducing downstream requirements. When applied to 384x and 1,536x member DropSynth gene libraries, BAR-CAT retrieved up to 122-fold enrichment for 12 targets and revealed practical limits imposed by sgRNA competition and library complexity, which now guide ongoing protocol scaling. …”

Data Sheet 1_The effects and mechanism of urease inhibitor and its combination with nitrification inhibitor on nitrous oxide emission across four soil types.docx by Churong Liu (11174070)

“…N fertilization significantly increased N₂O emissions by 5.1~99.9-fold and elevated CO₂ emissions by 13.6~65.4% across all soil types. The UI treatment decreased the peak of NO₂^– concentrations in two alkaline soils, while the UN treatment decreased both NO₂^– and NO₃^– concentrations in all four soils. …”

Presentation_1_Src Inhibition Attenuates Neuroinflammation and Protects Dopaminergic Neurons in Parkinson’s Disease Models.PDF by Hanyu Yang (8374881)

“…The results showed that inhibition of Src could significantly relieve microgliosis and decrease levels of inflammatory factors. Besides, inhibition of Src function reduced the loss of dopaminergic neurons and improved the motor behavior of the MPTP-treated mice. …”

<b>Insight into the effects of citric acid on the transformation of chemical</b><b> speciation</b><b> of Cd in</b> <b>biochar amended contaminated soil:</b> <b>Implications for bio... by Renhui Miao (18618601)

“…<p dir="ltr">Understanding the roles of low-molecular-weight organic acids (LMWOAs) in the transformation of chemical speciation of heavy metals in contaminated soil amended by biochar is of great significance for assessing the effectiveness of this carbonaceous amendment. …”

Data Sheet 1_Analysis of serum amino acids and tryptophan metabolites to predict hepatic encephalopathy in portal hypertension patients receiving a transjugular intrahepatic portal... by Li Bao (323211)

“…</p>Discussion<p>This study provided the theoretical suggestion to the clinical doctor which the level of serum TRP/KYN/QA of patients should be detected before TIPS, and It is of great significance for patient prognosis and reducing the occurrence of HE after TIPS.…”

Data Sheet 1_Anti-neoplastic effects of the antipsychotic drug penfluridol in preclinical prostate cancer models.pdf by Arjanneke F. van de Merbel (5809940)

“…</p>Results<p>Penfluridol significantly reduced the viability of a panel of human PCa cells, induced apoptosis by increasing caspase-3/7 levels and decreased the number of PCa stem cells in vitro. …”

Data Sheet 2_Anti-neoplastic effects of the antipsychotic drug penfluridol in preclinical prostate cancer models.docx by Arjanneke F. van de Merbel (5809940)

“…</p>Results<p>Penfluridol significantly reduced the viability of a panel of human PCa cells, induced apoptosis by increasing caspase-3/7 levels and decreased the number of PCa stem cells in vitro. …”

Data Sheet 1_Lipoxin A4 levels correlate with severity in a Spanish COVID-19 cohort: potential use of endogenous pro-resolving mediators as biomarkers.docx by Sergio Sánchez-García (16862127)

“…</p>Methods and results<p>In this study, we have analyzed by ELISA the serum levels of lipoxin A₄ (LXA₄) in a representative Spanish cohort. We found reduced levels in deceased patients when compared to mild or severe patients, concomitant with a decrease in the LXA₄ biosynthetic pathway and an increase in its degradation pathway. …”

Data from: '<b>From climate warming to accelerated cellular ageing: an experimental study in a wild passerine bird'</b> by Antoine Stier (3596369)

“…</i> non-significant decrease in the gene expression of telomerase and protective shelterin). …”

Smoluchowski temperature and Maxwell velocity slip impacts on magneto-radiative <i>Fe</i>₃<i>O</i>₄-<i>Al</i>₂<i>O</i>₃-<i>Cu</i>/<i>H</... by Gopinath Mandal (17767951)

“…However, the temperature is reduced greatly by the suction parameter <math><mi>S</mi></math> and Smoluchowski temperature slip parameter <math><mi>ε</mi></math>. …”

Table 8_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.xlsx by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Table 4_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.xlsx by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Image 4_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.tif by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Table 3_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.xlsx by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Image 3_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.tif by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Table 6_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.docx by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Table 2_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.docx by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Image 5_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.tif by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Image 2_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.tif by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”

Table 1_Comparative genomics and metabolomics reveal phytohormone production, nutrient acquisition, and osmotic stress tolerance in Azotobacter chroococcum W5.xlsx by M. Elakkya (21760439)

“…Under salt and osmotic stress, A. chroococcum W5 metabolomic profiling revealed adaptive responses, including elevated levels of osmoprotectants (proline, glycerol) and oxidative stress markers such as 2-hydroxyglutarate, while putrescine and glycine decreased.</p>Discussion<p>Our results show that the A. chroococcum W5 strain has great potential for the development of novel formulations. …”